MRI: Acquisition of a Multi-Modality Multi-Photon Digital Light Sheet Microscope in the Pacific Northwest to Support Bioengineering Research and Development

MRI：在太平洋西北部采购多模态多光子数字光片显微镜以支持生物工程研究和开发

• 批准号: 2018562
• 负责人: Matthew Fields
• 金额: $ 108.15万
• 依托单位: Montana State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2018562&HistoricalAwards=false
• 关键词: MRI; Acquisition; Multi; Modality; Photon

This award is made to Montana State University to acquire a uniquely configured Multi-Modality Multi-Photon Digital Fluorescence Microscope. The Center for Biofilm Engineering at Montana State University, where the requested instrument will be located, was originally established in 1990 as an NSF Engineering Research Center (ERC), and it has a central mission to advance the frontiers of health, energy, industry, and the environment through biofilm research, education, and outreach. From inception, the Center for Biofilm Engineering has taken a transdisciplinary approach to fundamental and applied research that continues today and that embodies the Montana State University land-grant mission for the integration of learning and discovery. Such research empowers students to apply fundamental knowledge to real-world challenges. Currently, one of the biggest challenges for engineering-based biological applications is the ability to noninvasively visualize highly replicated live bio-systems in real-time.To overcome challenges with conducting physiologically relevant research in natural and engineered systems, the Multi-Photon Digital Fluorescence Microscope will enable unprecedented image acquisition speeds, significantly reduce phototoxicity, provide high resolution label free identification of single cells, and increase penetration depths producing images more representative of intact biofilms. All of these advancements are integral to maintaining cell viability during imaging and will support research projects of faculty, staff, and students across three colleges (Engineering, Science, and Agriculture). The acquisition of this instrument will allow cutting-edge, real-time, high-sensitivity imaging of intact, complex biological samples and is, thus, ideally suited for biofilm systems.This project is jointly funded by CBET-MRI Program and the Established Program to Stimulate Competitive Research (EPSCoR) Program.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项授予蒙大拿州立大学，以获得独特配置的多模态多光子数字荧光显微镜。蒙大拿州立大学的生物膜工程中心（所需仪器将位于该中心）最初成立于1990年，是美国国家科学基金会工程研究中心（ERC），其中心使命是通过生物膜研究、教育和推广来推进健康、能源、工业和环境的前沿。从一开始，生物膜工程中心就采取跨学科的方法进行基础和应用研究，这种方法一直持续到今天，并体现了蒙大拿州立大学土地赠款的使命，即整合学习和发现。这样的研究使学生能够将基础知识应用于现实世界的挑战。目前，工程生物应用的最大挑战之一是能够实时无创地可视化高度复制的活生物系统。为了克服在自然和工程系统中进行生理相关研究的挑战，多光子数字荧光显微镜将实现前所未有的图像采集速度，显着降低光毒性，提供单细胞的高分辨率无标记识别，并增加穿透深度，产生更能代表完整生物膜的图像。所有这些进步对于在成像过程中保持细胞活力是不可或缺的，并将支持三所学院（工程，科学和农业）的教职员工和学生的研究项目。该仪器的获得将允许对完整的、复杂的生物样品进行尖端的、实时的、高灵敏度的成像，因此，非常适合生物膜系统。该项目由CBET-MRI计划和刺激竞争研究的既定计划（EPSCoR）联合资助该奖项反映了NSF的法定使命，并通过使用基金会的智力价值进行评估，被认为值得支持和更广泛的影响审查标准。

项目成果

Osteocyte Remodeling of the Lacunar-Canalicular System: What’s in a Name?

• DOI: 10.1007/s11914-022-00766-3
• 发表时间: 2022-12
• 期刊: Current Osteoporosis Reports
• 影响因子: 4.3
• 作者: C. Heveran;J. Boerckel