MRI: Acquisition of a multi-modal laser scanning confocal microscope to integrate research and teaching across spatial, evolutionary and ecological scales at WWU

MRI：购买多模态激光扫描共聚焦显微镜，以整合 WWU 跨空间、进化和生态尺度的研究和教学

• 批准号: 2019228
• 负责人: Domenico Galati
• 金额: $ 68.13万
• 依托单位: Western Washington University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-15 至 2023-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2019228&HistoricalAwards=false
• 关键词: MRI; Acquisition; multi; modal; laser

An award is made to Western Washington University (WWU) to purchase a microscope for high resolution quantitative analysis of biological specimens. The acquisition will facilitate interdisciplinary curriculum development, summer research programs for undergraduates, and STEM pipeline building between WWU and K-12 students in the Skagit Valley. Curricular developments will be focused on Course-based Undergraduate Research Experiences (CUREs) that will enable ~64 undergraduate students annually to conduct quantitative fluorescence microscopy research on biological specimens through the Biology, Chemistry, Behavioral Neuroscience departments and the Shannon Point Marine Science Center (SPMC). Datasets generated through CUREs will be developed into teaching tools for lecture courses, which will expose an additional ~400 students annually to quantitative microscopy data. Summer research programs will focus on training a diverse cohort of ~16 students annually to employ time-resolved fluorescence microscopy on specimens ranging in scale from individual proteins to multi-cellular organisms. The acquisition will expose traditionally underserved K-12 students to cutting edge quantitative microscopy through the Compass2Campus program. Collectively, this instrument will become a central resource for quantitative STEM training for the entire Skagit Valley.The scientific premise for this acquisition is focused on advancing 11 research programs from three academic departments (Biology, Chemistry, and Behavioral Neuroscience) and one national marine science center (SPMC). These research programs work on diverse biological problems that include: organelle assembly, plant development, cell signaling, marine larval development, neural synapse formation, endosymbiosis, protein biophysics, protein trafficking, DNA damage, gene expression, neural development, and aging. These research emphases span size scales that range from single molecules to multi-cellular organisms. These research emphases incorporate evolutionarily distant organisms that include protists, fungus, plants, insects and animals. The results of the scientific projects facilitated by this acquisition will be disseminated in peer-reviewed publications and at scientific meetings.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

授予西华盛顿大学（WWU）购买一台显微镜，用于生物标本的高分辨率定量分析。此次收购将促进跨学科课程开发，本科生暑期研究计划以及WWU和Skagit Valley的K-12学生之间的STEM管道建设。课程发展将集中在基于课程的本科生研究经验（CURES），这将使每年约64名本科生通过生物学，化学，行为神经科学部门和香农点海洋科学中心（SPMC）对生物标本进行定量荧光显微镜研究。通过CURE生成的数据集将被开发成讲座课程的教学工具，每年将有大约400名学生接触定量显微镜数据。暑期研究计划将侧重于每年培训约16名学生的多样化队列，以在从单个蛋白质到多细胞生物体的样本上采用时间分辨荧光显微镜。此次收购将使传统上服务不足的K-12学生通过Compass 2Campus计划接触到尖端的定量显微镜。总的来说，这台仪器将成为整个斯卡吉特山谷定量STEM培训的核心资源。此次收购的科学前提是专注于推进来自三个学术部门（生物学，化学和行为神经科学）和一个国家海洋科学中心（SPMC）的11个研究项目。这些研究项目致力于研究各种生物学问题，包括：细胞器组装，植物发育，细胞信号传导，海洋幼虫发育，神经突触形成，内共生，蛋白质生物物理学，蛋白质运输，DNA损伤，基因表达，神经发育和衰老。这些研究重点跨越从单分子到多细胞生物体的大小尺度。这些研究重点包括进化上遥远的生物，包括原生生物，真菌，植物，昆虫和动物。此次收购所促进的科学项目的结果将在同行评审的出版物和科学会议上传播。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Immunofluorescence Microscopy

免疫荧光显微镜

• DOI: 10.1002/cpz1.842
• 发表时间: 2023
• 期刊: Current Protocols
• 作者: Galati, Domenico F.;Asai, David J.
• 通讯作者: Asai, David J.