Elucidating and Computing Metabolic Networks for Co-Valorization of Cellulose and Lignin Derivatives

阐明和计算纤维素和木质素衍生物共同增值的代谢网络

• 批准号: 2022854
• 负责人: Ludmilla Aristilde
• 金额: $ 49万
• 依托单位: Northwestern University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2022854&HistoricalAwards=false
• 关键词: Elucidating; Computing; Metabolic; Networks; Co

Conversion of lignin and cellulose from trees and plants into valuable products could reduce our reliance on non-renewable feedstocks such as petroleum and natural gas. Pseudomonas are species of soil bacteria with robust and varied metabolisms. As a result, they can produce a variety of potentially important molecules. This project will develop a detailed map of the pathways involved in processing lignin and cellulose by Pseudomonas. The regulation of these pathways will also be studied. The project will extend research opportunities to undergraduates and will engage women with STEM outreach activities at a local high school. The project aims to understand the mechanisms of the metabolic networks in wild-type and engineered Pseudomonas putida strains. Mixed-substrate utilization of sugars from cellulose and hemicellulose, and aromatic substrates from lignin will be the primary focus. There are three specific objectives. First, elucidate the metabolic architecture for simultaneous processing of carbons derived from cellulose and lignin monomers. Second, analyze the metabolic network fluxes towards relevant biosynthetic pathways quantitatively. Third, identify the metabolic nodes under regulatory control in the network. The project will employ a combination of stable isotope-assisted metabolomics, proteomics profiling, genome-scale quantitative flux modeling, and in silico predictions of optimal metabolic programming. The long-term goal of this research is to unravel metabolic pathway limitations to converting lignocellulosic feedstock derivatives to value-added building blocks.This project is being supported jointly by the Cellular and Biochemical Engineering (ENG/CBET) and the Systems and Synthetic Biology (BIO/MCB) Programs.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

将木质素和纤维素从树木和植物转化为有价值的产品可以减少我们对不可再生原料（例如石油和天然气）的依赖。假单胞菌是土壤细菌的种类，具有鲁棒和多样性的代谢。结果，它们可以产生各种潜在的重要分子。该项目将开发一张详细的图表，以通过假单胞菌加工木质素和纤维素涉及的途径。这些途径的调节也将进行研究。该项目将扩大研究机会到本科生，并将在当地一所高中与妇女进行STEM外展活动。该项目旨在了解野生型和工程化假单胞菌菌株中代谢网络的机制。纤维素和半纤维素的糖的混合基层利用，以及木质素的芳香族底物将是主要重点。有三个特定目标。首先，阐明了代谢结构，用于同时加工源自纤维素和木质素单体的碳。其次，分析代谢网络通量，定量地针对相关的生物合成途径。第三，确定网络调节控制下的代谢节点。该项目将采用稳定的同位素辅助代谢组学，蛋白质组学分析，基因组尺度定量通量建模以及最佳代谢编程的硅预测。这项研究的长期目标是阐明代谢途径的局限性，以将木质纤维素原料衍生物转换为增值的基础。基金会的智力优点和更广泛的影响评论标准。