I-Corps: A Super-Sensitive, Real-Time Sensor for Reactive Oxygen Species (ROS)

I-Corps项目更广泛的影响/商业潜力是开发一种实时检测活性氧（ROS）的传感器，活性氧是一种自由基，可导致衰老、癌症和神经退行性疾病。目前，ROS没有标准的测量方案，导致生物标志物的解释和临床诊断不一致。开发了一种高灵敏度，实时的ROS传感器，与现有方法相比具有显着优势。这种传感器可以改善目前的临床和美容实践，并有助于预防与自由基有关的疾病。这项技术还可以为测量自由基提供一个工业标准协议，这将有助于获得更可靠的结果。该技术还可用于食品包装和食品配送行业，用于监测食品中的化学和微生物污染。此外，该传感器还可以在环境方面应用，为污染地下水修复提供高级氧化过程中自由基的更好控制。这个I-Corps项目的基础是开发一种超灵敏的传感器，用于检测活性氧（ROS）。该技术由一种复合材料修饰的电极组成，该复合材料由超小型氧化铈纳米颗粒固定在高导电性碳载体上。利用氧化铈对羟基自由基的强亲和力，研制了一种实时检测的复合传感器。利用样品与传感器相互作用产生的阻抗信号检测ROS的类型和浓度，羟基自由基的检测限（LOD）低至0.006 mM，而其他方法的LOD为0.1-10 mM。使用3D打印机设计和制造了一种手持式检测设备，并已成功地在体外对各种液体和组织样本进行了测试。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

The broader impact/commercial potential of this I-Corps project is the development of a sensor for the real-time detection of reactive oxygen species (ROS), which are free radicals that can lead into aging, cancer, and neurodegenerative diseases. Currently, there is no standard measuring protocol for ROS, causing mixed interpretation and clinical diagnoses of biomarkers. A highly sensitive, real-time sensor for ROS was developed with significant advantages over existing methods. This sensor may improve current clinical and cosmetic practices and help in the prevention of diseases associated with free radicals. This technology also may provide an industrial standard protocol for measuring free radicals, which would contribute to more reliable results. This technology also may be used in food packaging and food distributing industries for monitoring chemical and microbial contamination in food. In addition, it also may have environmental applications, as the sensor could help to provide better control of the free radicals in the advanced oxidation process for contaminated groundwater remediation. This I-Corps project is based on the development of a super-sensitive sensor for the detection of reactive oxygen species (ROS). The technology consists of an electrode modified with a composite comprising ultra-small cerium oxide nanoparticles anchored to a highly conductive carbon support. The strong affinity of cerium oxide toward hydroxyl free radicals was used to develop a composite sensor for real-time detection. The impedance signal generated from the interaction between the sample and the sensor is used to detect the type and concentration of ROS, with a limit of detection (LOD) as low as 0.006 mM of hydroxyl free radicals, as compared to LODs of 0.1-10 mM with other methods. A hand-held detection device has been designed and fabricated using a 3D printer, and this has been successfully tested in various liquid and tissue samples in vitro.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.