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ECA-PGR: Transcriptional Regulation and Gene Networks Underlying Viral Recognition of Insect Vectors in Host Plants

ECA-PGR：宿主植物中昆虫载体病毒识别的转录调控和基因网络

基本信息

批准号：
2026068
负责人：
Clare Casteel
金额：
$ 131.59万
依托单位：
Cornell University
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2019
资助国家：
美国
起止时间：
2019-10-01 至 2025-06-30
项目状态：
未结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2026068&HistoricalAwards=false
关键词：
ECA PGR Transcriptional Regulation Gene

项目摘要

Viral infections in crop plants can cause major losses in crop productivity. Despite more than a century of research on plant-virus interactions, control of viral infection remains a challenge to plant health. Unlike most bacterial and fungal plant pests, most viruses depend on insects who serve as "vectors" to transmit the virus to the plant cell, often through sucking mouthparts. Thus, a complex three-way relationship develops in which the host plant mounts an immune response that is countered by the virus and its insect vector. Understanding how and when this three-way interaction occurs is thus essential to identify control points of the viral disease. The goals of this research program are to investigate plant responses to infection and to uncover unique collections of genes and biochemical pathways that drive plant-virus-insect interactions in tomato. The results will provide candidate genes for engineering new strategies of virus management, and will establish the tomato-Potyvirus-aphid system as a model for studying three-way disease interactions. The research also provides novel insight into how the natural variation of viral disease is mediated within and across domesticated and wild tomato. High school and undergraduate students will participate directly in research to learn about the genomic-level science underlying viral disease in plants. The project will also encourage cross-disciplinary communication in plant genomics by hosting a summer discussion group at the University of California-Davis focused on plant genomics research.Nearly every major cropping system faces threats from plant-infecting viruses, and most plant viruses rely on insect vectors for transmission. Although numerous studies have shown that viruses can manipulate plant metabolism to promote viral acquisition and transmission by vectors, few have examined the underlying molecular mechanisms mediating plant-virus-vector interactions and none have developed a system-level understanding of this process. This work builds on the recent discovery that plant viruses respond actively to the presence of insect vectors via host plant-derived signals, promoting insect performance and transmission through changes in plant chemistry. The aim of this project is to develop a detailed understanding of the genes and pathways that underlie viral "recognition" of insect vectors and activation of processes within the host plant that facilitate transmission by insect vectors. Transcript profiling, metabolomics, and bioinformatics will be used to develop a co-expression network and identify key regulators of this phenomenon. Introgression-mapping lines in combination with knockouts will be used to functionally characterize key regulators mediating plant-virus-vector interactions within and across plant species. All data and resources generated in this project will be made accessible to the public through the project website and through long-term data repositories.

农作物的病毒感染会导致农作物生产力的重大损失。尽管对植物与病毒相互作用的研究已有一个多世纪的历史，但病毒感染的控制仍然是植物健康的一个挑战。与大多数细菌和真菌植物害虫不同，大多数病毒依赖昆虫作为“载体”，通常通过吸吮口器将病毒传播到植物细胞。因此，一种复杂的三向关系就形成了，其中宿主植物发起免疫反应，而病毒及其昆虫载体会抵抗这种免疫反应。因此，了解这种三向相互作用如何以及何时发生对于确定病毒性疾病的控制点至关重要。该研究计划的目标是调查植物对感染的反应，并揭示驱动番茄中植物-病毒-昆虫相互作用的独特基因集合和生化途径。研究结果将为设计新的病毒管理策略提供候选基因，并将建立番茄-马铃薯Y病毒-蚜虫系统作为研究三向疾病相互作用的模型。该研究还提供了关于病毒性疾病的自然变异如何在驯化和野生番茄内部和之间介导的新见解。高中生和本科生将直接参与研究，了解植物病毒性疾病背后的基因组科学。该项目还将通过在加州大学戴维斯分校主办一个专注于植物基因组学研究的夏季讨论小组，鼓励植物基因组学的跨学科交流。几乎每个主要种植系统都面临植物感染病毒的威胁，并且大多数植物病毒依赖昆虫媒介进行传播。尽管大量研究表明病毒可以操纵植物代谢，以促进病毒通过载体获得和传播，但很少有人研究介导植物-病毒-载体相互作用的潜在分子机制，也没有人对这一过程有系统水平的理解。这项工作建立在最近的发现基础上，即植物病毒通过宿主植物来源的信号对昆虫载体的存在做出积极反应，通过植物化学的变化促进昆虫的表现和传播。该项目的目的是详细了解病毒“识别”昆虫媒介的基因和途径以及宿主植物内促进昆虫媒介传播的过程的激活。转录谱分析、代谢组学和生物信息学将用于开发共表达网络并确定这种现象的关键调节因子。基因渗入作图系与敲除相结合，将用于在功能上表征介导植物物种内和跨植物物种之间植物-病毒-载体相互作用的关键调节因子。该项目产生的所有数据和资源将通过项目网站和长期数据存储库向公众开放。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Spatiotemporal dynamics of the tomato fruit transcriptome under prolonged water stress

长期水分胁迫下番茄果实转录组的时空动态

DOI：
10.1093/plphys/kiac445
发表时间：
2022
期刊：
Plant Physiology
影响因子：
7.4
作者：
Nicolas, Philippe;Shinozaki, Yoshihito;Powell, Adrian;Philippe, Glenn;Snyder, Stephen I.;Bao, Kan;Zheng, Yi;Xu, Yimin;Courtney, Lance;Vrebalov, Julia
通讯作者：
Vrebalov, Julia

Acylsugars protect Nicotiana benthamiana against insect herbivory and desiccation