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Transitions: Experimental Evolutionary Cell Biology

转变：实验进化细胞生物学

基本信息

批准号：
2027389
负责人：
Holly Goodson
金额：
$ 72.85万
依托单位：
University of Notre Dame
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2020
资助国家：
美国
起止时间：
2020-07-01 至 2024-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2027389&HistoricalAwards=false
关键词：
Transitions Experimental Evolutionary Cell Biology

项目摘要

Evolutionary cell biology is an emerging field that has the potential to provide insight into the myriad ways cells work along with the evolutionary processes and constraints which molds their structures and functions. The goal of this project is to help provide valuable training to a cell biology group, which will enable them to tackle important problems in this emerging field. Specifically, the group will gain expertise in growing cells of an ecologically important red algae, but one in which cell biology understanding is limited. In an interdisciplinary collaboration, insight will be gained into how these algae have evolved without the benefit of what is typically considered to be essential proteins. The PI and the collaborating group will use the resulting broad perspectives and interdisciplinary tools to identify proteins and mechanisms that are common across divergent cellular organisms, helping to illuminate basic cell biological and evolutionary processes. Broader Impact activities will include the training of high school students, undergraduate and graduate students in research methods and additional outreach work will target an older generation on the interrelatedness of science. This research consists of two projects. Project 1 has two goals: a) to experimentally test hypotheses developed from phylogenetic analysis of how the red alga Porphyra umbilicalis accomplishes core biological processes (e.g., cell movement, membrane transport, cell division) without key cytoskeletal proteins; b) to deepen our understanding of the cytoskeletal evolution in red algae (and plants more broadly) by conducting an initial study of the cytoskeleton in Rhodelphis limneticus, a motile and predatory organism that belongs to a phylum sister to red algae. In Project 2, in vitro evolution experiments will be carried out, with the goal of testing the hypothesis (developed through discussions with collaborators in population genetics and mathematical biology) that constraints imposed by nutrient availability affect the rate of adaptation to non-nutrient stresses. This pilot project has relevance for development of resistance to pesticides and drugs. Together, these parallel projects are designed to build a solid foundation for future research in experimental evolutionary cell biology.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

进化细胞生物学是一个新兴的领域，它有可能提供对细胞沿着进化过程和塑造其结构和功能的限制的无数方式的洞察。这个项目的目标是帮助提供有价值的培训，以细胞生物学组，这将使他们能够解决这个新兴领域的重要问题。具体来说，该小组将获得在生态上重要的红藻细胞生长的专业知识，但其中细胞生物学的理解是有限的。在跨学科的合作中，将深入了解这些藻类是如何在没有通常被认为是必需蛋白质的情况下进化的。 PI和合作小组将利用由此产生的广泛视角和跨学科工具来识别不同细胞生物体中常见的蛋白质和机制，帮助阐明基本的细胞生物学和进化过程。 “更广泛的影响”活动将包括对高中生、本科生和研究生进行研究方法方面的培训，另外的外联工作将针对老一代人，使他们了解科学的相互关联性。本研究包括两个项目。项目1有两个目标：a）通过实验测试从系统发育分析中发展出来的关于红紫菜如何完成核心生物学过程的假设（例如，细胞运动、膜运输、细胞分裂）而没有关键的细胞骨架蛋白; B）通过对Rhodelphis limneticus（一种属于红藻姐妹门的能动和捕食性生物体）的细胞骨架进行初步研究，加深我们对红藻（和更广泛的植物）中细胞骨架进化的理解。在项目2中，将进行体外进化实验，目的是检验（通过与群体遗传学和数学生物学合作者讨论而提出的）假设，即营养供应的限制影响对非营养压力的适应速度。这一试点项目与发展对农药和药物的抗药性有关。这些平行项目旨在为未来的实验进化细胞生物学研究奠定坚实的基础。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。