Transitions: Experimental Evolutionary Cell Biology

转变：实验进化细胞生物学

基本信息

批准号：
2027389
负责人：
Holly Goodson
金额：
$ 72.85万
依托单位：
University of Notre Dame
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2020
资助国家：
美国
起止时间：
2020-07-01 至 2024-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2027389&HistoricalAwards=false
关键词：
Transitions Experimental Evolutionary Cell Biology

项目摘要

Evolutionary cell biology is an emerging field that has the potential to provide insight into the myriad ways cells work along with the evolutionary processes and constraints which molds their structures and functions. The goal of this project is to help provide valuable training to a cell biology group, which will enable them to tackle important problems in this emerging field. Specifically, the group will gain expertise in growing cells of an ecologically important red algae, but one in which cell biology understanding is limited. In an interdisciplinary collaboration, insight will be gained into how these algae have evolved without the benefit of what is typically considered to be essential proteins. The PI and the collaborating group will use the resulting broad perspectives and interdisciplinary tools to identify proteins and mechanisms that are common across divergent cellular organisms, helping to illuminate basic cell biological and evolutionary processes. Broader Impact activities will include the training of high school students, undergraduate and graduate students in research methods and additional outreach work will target an older generation on the interrelatedness of science. This research consists of two projects. Project 1 has two goals: a) to experimentally test hypotheses developed from phylogenetic analysis of how the red alga Porphyra umbilicalis accomplishes core biological processes (e.g., cell movement, membrane transport, cell division) without key cytoskeletal proteins; b) to deepen our understanding of the cytoskeletal evolution in red algae (and plants more broadly) by conducting an initial study of the cytoskeleton in Rhodelphis limneticus, a motile and predatory organism that belongs to a phylum sister to red algae. In Project 2, in vitro evolution experiments will be carried out, with the goal of testing the hypothesis (developed through discussions with collaborators in population genetics and mathematical biology) that constraints imposed by nutrient availability affect the rate of adaptation to non-nutrient stresses. This pilot project has relevance for development of resistance to pesticides and drugs. Together, these parallel projects are designed to build a solid foundation for future research in experimental evolutionary cell biology.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

进化细胞生物学是一个新兴领域，有潜力可以深入了解细胞的工作方式以及塑造其结构和功能的进化过程和约束。该项目的目的是为细胞生物学小组提供宝贵的培训，这将使他们能够解决这个新兴领域的重要问题。具体而言，该小组将在生态上重要的红藻的细胞中获得专业知识，但了解细胞生物学的理解是有限的。在跨学科的合作中，将获得这些藻类如何发展的见解，而没有通常被认为是必不可少的蛋白质的好处。 PI和协作小组将使用由此产生的广泛观点和跨学科工具来识别在不同细胞生物中常见的蛋白质和机制，从而有助于阐明基本的细胞生物学和进化过程。更广泛的影响活动将包括对研究方法的高中生，本科生和研究生的培训，而额外的外展工作将针对老年来的科学相互关联。这项研究由两个项目组成。项目1有两个目标：a）从系统发育分析进行实验检验的假设，该假设是关于红色藻类斑马脐带如何完成核心生物过程（例如细胞运动，膜转运，细胞分裂）而没有关键细胞骨架蛋白质的； b）通过对Rhodelphis limneticus中的细胞骨架进行初步研究，我们对红藻的细胞骨架进化（以及更广泛的植物）的理解，这是一种属于红色藻类的门虫姐妹。在项目2中，将进行体外进化实验，目的是检验假设（通过与人口遗传学和数学生物学的协作者进行讨论，从营养可用性施加的限制会影响对非营养应力的适应率。该试点项目与开发对农药和药物的抗性相关。这些平行的项目共同旨在为实验进化细胞生物学的未来研究奠定坚实的基础。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛影响的评估标准通过评估来支持的。