Collaborative Research: Use of genome enabled tools to understand symbiosis

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。合作研究：利用基因组工具来理解共生关系鱿鱼-费氏弧菌共生关系已经成为研究细菌在动物体内定植的重要模型。弧菌属中几个基因组测序的可用性，使其成为一个比以往更强大的系统，用于阐明复杂的基因网络，使细菌的不同生活方式成为可能。对于这个集研究和教育于一体的分子遗传学项目，研究人员将构建在费氏弧菌中存在的4000多个细菌基因中每个基因都有缺陷的突变细菌。然后，这个发现工具将被六个几乎全部由本科研究人员组成的研究小组用于表征与动物定植相关的高通量分析中的细菌特性。这个突变体文库，以及来自多个并行分析的数据，将提供一个由基因索引的高分辨率功能相关数据集网络，这将在整个基因组的背景下提供前所未有的基因功能视图。这个突变体库将对公众开放，用于生物信息学分析，从而为下一代实验提供动力。研究越来越多地涉及高通量分子遗传学和基于基因组学的发现策略，这往往需要大型团队的协调。因此，参与该项目的大量学生将为21世纪的科学事业做好准备。

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).Eric StabbCollaborative Research: Use of genome enabled tools to understand symbiosis IOS- 0841480The squid-Vibrio fischeri symbiosis has emerged as an engaging and important model for studying animal colonization by bacteria. The availability of several sequenced genomes in the genus Vibrio, has made this a more powerful system than ever for elucidating the complex gene networks that enable the contrasting lifestyles of bacteria. For this molecular genetics project which integrates research and education, the investigators will construct mutant bacteria with defects in each of the more than four thousand bacterial genes present in V. fischeri. This tool for discovery will then be used to characterize bacterial traits relevant to animal colonization in high-throughput assays by six research teams comprised almost entirely of undergraduate researchers. This library of mutants, along with data from the multiple parallel assays, will provide a high-resolution network of functionally-related datasets indexed by gene that will provide an unprecedented view of gene function in the context of the entire genomes. This library of mutants will be publicly available for bioinformatics analysis, and thus will fuel the next generation of experiments. Research increasingly involves high-throughput molecular genetic and genomics-based strategies for discovery that often necessitate the coordination of large teams. Therefore, the large number of students participating in this project will be well prepared for science careers in the 21st century.