Bacterial RNA thermometers: From individual examples to global analyses

细菌 RNA 温度计：从个体实例到全局分析

• 批准号: 244588142
• 负责人: Professor Dr. Franz Narberhaus
• 金额: --
• 依托单位: Lehrstuhl für Biologie der Mikroorganismen
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/244588142?language=en
• 关键词: Bacterial; RNA; thermometers; individual; examples

Structured RNA regions influence every step in the gene expression program. Many bacterial heat shock and virulence genes are regulated by RNA thermometers (RNATs), which are molecular zippers that control translation initiation. They adopt a structure that blocks the ribosome binding site at low temperatures. A temperature upshift to 37°C (virulence genes) or higher (heat shock genes) destabilizes the structure and permits translation.In the previous funding period, we have studied various natural and synthetic RNATs in molecular detail. By using a combination of in vitro structure probing and next-generation sequencing we mapped the entire RNA structurome of the human pathogen Yersinia pseudotuberculosis at three different temperatures. We now want to take advantage of this comprehensive data resource and characterize two distinct types of regulation. (i) Following the initial characterization of more than 25 novel RNATs, the structure and physiological function of a number of these regulatory elements will be evaluated. We are particularly interested in the virulence factor CnfY, and components of the oxidative stress response and type III secretion system. (ii) The RNA structurome results suggest a regulatory concept, in which several small regulatory RNAs undergo temperature-dependent conformational changes that mask or unmask their target interaction region. We will further evaluate this observation and study its effect on target gene regulation. In a third work package, we plan to capitalize on a previously developed in vivo RNA structuromics approach with the goal to learn how RNAs fold in the living cell.

结构化的RNA区域影响基因表达程序的每个步骤。许多细菌热休克和病毒基因受RNA温度计（RNAT）调节，RNA温度计（RNAT）是控制翻译倡议的分子拉链。他们采用一种在低温下阻断核糖体结合位点的结构。温度上升至37°C（毒力基因）或更高（热休克基因）破坏了结构并允许翻译。在上一个资金期间，我们已经以分子细节研究了各种自然和合成RNAT。通过使用体外结构探测和下一代测序的组合，我们在三种不同的温度下绘制了人类病原体耶尔森氏病的整个RNA结构。现在，我们想利用这种全面的数据资源并表征两种不同类型的法规。 （i）将在25多个新型RNAT的初始表征之后，将评估许多这些调节元素的结构和物理功能。我们对病毒因子CNFY以及氧化物应激反应和III型分泌系统的成分特别感兴趣。 （ii）RNA结构的结果提出了一个调节概念，其中几个小的调节RNA经历了温度依赖的会议变化，以掩盖或揭示其目标相互作用区域。我们将进一步评估这一观察结果，并研究其对靶基因调节的影响。在第三个工作包中，我们计划利用先前开发的体内RNA结构方法，以了解RNA如何在活细胞中折叠。