The role of small RNAs in physiology and virulence of Agrobacterium tumefaciens

小RNA在根癌农杆菌生理学和毒力中的作用

• 批准号: 169862773
• 负责人: Professor Dr. Franz Narberhaus
• 金额: --
• 依托单位: Lehrstuhl für Biologie der Mikroorganismen
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/169862773?language=en
• 关键词: role; small; RNAs; physiology; virulence

Small regulatory RNAs (sRNAs) play an important role in gene regulation in all domains of life. We aim at the identification and characterization of sRNAs from the plant-pathogen Agrobacterium tumefaciens, a natural genetic engineer that transfers part of its own genome into plants to re-program their metabolism resulting in tumor formation. A combined bio-computational and deep sequencing approach revealed several hundred sRNAs in A. tumefaciens. The structure and function of selected candidates will be studied in detail. To reveal fundamental housekeeping functions, preference will be given to an overlapping set of sRNAs that were identified by both strategies. Of particular interest are A. tumefaciens sRNAs from the tumor-inducing Ti plasmid. At least one sRNA is massively induced under virulence conditions. Its structure, target gene(s) and role in virulence will be addressed. microRNAs (miRNAs) might be expressed from the T-DNA that is delivered from the bacterium to the plant. The expression of computationally predicted miRNA precursors will be monitored in Agrobacterium and in planta. Identification of miRNAs in the host plant will be attempted by a deep sequencing approach.

小调节性RNA（SRNA）在生命的所有领域的基因调节中都起着重要作用。我们旨在鉴定和表征来自植物病原体农杆菌Tumefaciens的SRNA，这是一种自然遗传工程师，将其一部分基因组的一部分转移到植物中，以重新编程其代谢，从而导致肿瘤形成。合并的生物计算和深度测序方法揭示了A. tumefaciens中的数百个SRNA。将详细研究选定候选人的结构和功能。为了揭示基本的家政功能，将优先考虑两种策略确定的一组重叠的SRNA。 特别令人感兴趣的是来自肿瘤诱导Ti质粒的Tumefaciens srNA。在毒力条件下，至少有一个SRNA被大量诱导。它的结构，靶基因和在毒力中的作用将得到解决。 MicroRNA（miRNA）可以从从细菌传递到植物的T-DNA表达。计算预测的miRNA前体的表达将在农业杆菌和植物中进行监测。宿主植物中miRNA的识别将通过深层测序方法尝试。