CAREER: Parallel Two-Dimensional Liquid Chromatography Utilizing Capillary Columns

职业：利用毛细管柱的平行二维液相色谱

• 批准号: 2045023
• 负责人: James Grinias
• 金额: $ 52.5万
• 依托单位: Rowan University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-01-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2045023&HistoricalAwards=false
• 关键词: CAREER; Parallel; Two; Dimensional; Liquid

With support from the Chemical Measurement and Imaging Program in the Division of Chemistry, Dr. James Grinias at Rowan University will study new two-dimensional liquid chromatography (2D-LC) techniques used to measure critical quality attributes in small molecule-protein materials. Biopharmaceuticals composed of small molecules and proteins are increasing in importance within the pharmaceutical industry, because of their potential for addressing the shortcomings of other types of pharmaceutical agents. Chemical analysts within this industry need new measurement tools to ensure the content and purity of these small molecule-protein materials. Dr. James Grinias of Rowan University uses liquid chromatography to help separate and measure the individual molecules within complex mixtures that contain both active pharmaceutical ingredients and impurities. This project focuses on the creation of a two-dimensional liquid separation technique that can simultaneously measure multiple attributes of such complex mixtures, which helps ensure the purity of small molecule-protein materials. A group of chemical separation experts from multiple industries are collaborating with Dr. Grinias to provide feedback on high-value technical skills that are required in modern chemical measurement science laboratories in industrial settings. A new outreach program teaches these critical skills to nontraditional students by use of remote learning strategies, thereby helping them obtain careers in this field. With this award, the Chemical Measurement and Imaging Program is funding Dr. James Grinias at Rowan University to study new two-dimensional liquid chromatography (2D-LC) techniques used to measure critical quality attributes in small molecule-protein materials. Current strategies for characterizing these attributes in small molecule-protein materials require multiple, different chromatographic methods and columns, which can be a slow and arduous process. Dr. Grinias is overcoming this issue through the use of a parallel 2D-LC technique that utilizes multiple capillary LC columns in the second dimension to simultaneously perform multiple separation methods. This approach increases the throughput of the analyses, reduces the amount of chemical waste that is generated based on the small solvent volumes used, and limits the risks of sample handling for analysts through improvements in method automation. This CAREER Award project establishes this new technique by developing a multi-pump, capillary-scale system for parallel 2D-LC, coupling absorbance and mass spectrometric detection for selective channel monitoring, and demonstrating the parallel completion of multi-attribute methodology. These research objectives are connected to an educational plan that focuses on the identification of important job skills by industry leaders and the creation of a remote learning curriculum designed to teach these skills.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学系化学测量和成像计划的支持下，罗文大学的James Grinias博士将研究用于测量小分子蛋白质材料的关键质量属性的新的二维液相色谱(2D-LC)技术。由小分子和蛋白质组成的生物制药在制药业中的重要性日益增加，因为它们有潜力解决其他类型药剂的缺点。该行业的化学分析师需要新的测量工具来确保这些小分子蛋白质材料的含量和纯度。罗文大学的詹姆斯·格里尼亚斯博士使用高效液相色谱来帮助分离和测量含有活性药物成分和杂质的复杂混合物中的单个分子。该项目致力于创造一种二维液体分离技术，可以同时测量这种复杂混合物的多种属性，这有助于确保小分子蛋白质材料的纯度。来自多个行业的一组化学分离专家正在与Grinias博士合作，就工业环境中现代化学测量科学实验室所需的高价值技术技能提供反馈。一项新的推广计划通过使用远程学习策略向非传统学生传授这些关键技能，从而帮助他们在这一领域获得职业生涯。通过这一奖项，化学测量和成像计划资助罗文大学的James Grinias博士研究用于测量小分子蛋白质材料的关键质量属性的新的二维液相色谱(2D-LC)技术。目前在小分子蛋白质材料中表征这些属性的策略需要多种不同的色谱方法和色谱柱，这可能是一个缓慢而艰巨的过程。Grinias博士正在通过使用并行2D-LC技术克服这一问题，该技术利用第二维的多个毛细管LC柱同时执行多种分离方法。这种方法增加了分析的吞吐量，减少了因使用少量溶剂而产生的化学废物量，并通过改进方法自动化来限制分析人员处理样品的风险。这项职业奖项目通过开发一种用于并行2D-LC的多泵、毛细管尺度系统、用于选择性通道监测的耦合吸光度和质谱学检测来建立这项新技术，并展示了多属性方法的并行完成。这些研究目标与一项教育计划相关联，该计划侧重于确定行业领导者的重要工作技能，并创建旨在教授这些技能的远程学习课程。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Development of a dual-electrospray ionization source with in-line absorbance-based voltage control

开发具有在线吸光度电压控制的双电喷雾电离源

• DOI: 10.1007/s00216-023-04564-3
• 发表时间: 2023
• 期刊: Analytical and Bioanalytical Chemistry
• 影响因子: 4.3
• 作者: Foster, Samuel W.;Parker, Deklin;Piccolo, Christopher;Will, Matthew;Grinias, James P.
• 通讯作者: Grinias, James P.

Use of Portable Capillary Liquid Chromatography for Common Educational Demonstrations Involving Separations

使用便携式毛细管液相色谱进行涉及分离的常见教育演示

• DOI: 10.1021/acs.jchemed.1c00342
• 发表时间: 2021
• 期刊: Journal of Chemical Education
• 影响因子: 3
• 作者: Pham, Michelle;Foster, Samuel W.;Kurre, Sangeeta;Hunter, Rebecca A.;Grinias, James P.
• 通讯作者: Grinias, James P.

Measurement of optimal flow rate in gradient elution liquid chromatography

• DOI: 10.1016/j.chroma.2021.462645
• 发表时间: 2021-10-31
• 期刊: JOURNAL OF CHROMATOGRAPHY A
• 影响因子: 4.1
• 作者: Foster, Samuel W.;Wright, Nicholas;Blumberg, Leonid M.
• 通讯作者: Blumberg, Leonid M.

LCGC and ACS Subdivision on Chromatography and Separations Chemistry Survey 2023: What Skills Do New Analytical Chemistry Employees Need?

LCGC 和 ACS 分部色谱与分离化学调查 2023：新分析化学员工需要哪些技能？

• DOI: 10.56530/lcgc.na.uc7884m3
• 发表时间: 2023
• 期刊: LCGC North America
• 影响因子: 0.9
• 作者: Grinias, James;Smith, Trevor;Kovarik, Michelle
• 通讯作者: Kovarik, Michelle

Implementing 1.5 mm internal diameter columns into analytical workflows

在分析工作流程中实施 1.5 mm 内径色谱柱

• DOI: 10.1016/j.chroma.2022.463207
• 发表时间: 2022
• 期刊: Journal of Chromatography A
• 影响因子: 4.1
• 作者: Libert, Benjamin P.;Godinho, Justin M.;Foster, Samuel W.;Grinias, James P.;Boyes, Barry E.
• 通讯作者: Boyes, Barry E.