Plant viruses for three dimensional actuators

用于三维执行器的植物病毒

• 批准号: 245895196
• 负责人: Professorin Dr. Gabriele Krczal
• 金额: --
• 依托单位: AlPlanta Institute for Plant Research
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/245895196?language=en
• 关键词: Plant; viruses; three; dimensional; actuators

Biological systems commonly show selfassembly to form complex structures. It would be also of interest for technical applications, if complex nano- and microstructures could be produced by selfassembly to reduce production time and costs. In this project the selfassembly of genetically engineered plant viruses will be analyzed and utilized. The surface properties of plant viruses can be modified to a large extent and they show good crystallization properties. On the surface of recombinant viral capsids, molecules can be presented which show specific interactions with e.g. metals or metal ions, such as His-tags with Co and Ni ions or cysteins with Au and Ag. However, for technical applications up to now, no extended two-dimensional crystals can be formed on hard surfaces despite of the good crystallization properties.Preliminary tests with spherical tomato bushy stunt viruses, genetically engineered to present either positively or negatively charged amino acid chains, resulted, however, in the formation of two-dimensional crystals in considerable size. In the present project this growth will be further optimized and in consequence more complex structures will be established. In particular the link between Ni ions and histidin will be exlpoited in several ways. This comprises the binding of the virus to the substrate as well as the modification of the virus surface, e.g. through metallization mediated by histidin and other side chains. In addition, new functionalizations of the outer capsid surface will be proven and systems for the loading of the capsid with different compounds iwill be established.Our general objective is on the one hand the deeper understanding of the underlying principles in the selfassembly process of (modified) spherical viruses. On the other hand we want to explore possible applications, in particular those, in which ordered arrays of metals are of interest, e.g. in plasmonics or spintronics.

生物系统通常表现出自我组装，形成复杂的结构。如果可以通过自组装来生产复杂的纳米结构和微结构，以减少生产时间和成本，这对技术应用也是有意义的。本计画将分析及利用基因工程植物病毒之自我组装。植物病毒的表面性质可以在很大程度上被改变，并且它们显示出良好的结晶性质。在重组病毒衣壳的表面上，可以存在显示出与例如金属或金属离子的特异性相互作用的分子，例如具有Co和Ni离子的His-标签或具有Au和Ag的半胱氨酸。然而，到目前为止，对于技术应用，没有扩展的二维晶体可以在硬表面上形成，尽管良好的结晶性能。与球形番茄丛矮病毒，基因工程，以呈现带正电荷或负电荷的氨基酸链的初步测试，结果，然而，在相当大的尺寸的二维晶体的形成。在本项目中，这种增长将进一步优化，因此将建立更复杂的结构。特别是镍离子和组氨酸之间的联系将exlpoited在几个方面。这包括病毒与底物的结合以及病毒表面的修饰，例如通过组氨酸和其它侧链介导的金属化。此外，新的功能化的外衣壳表面将被证明和系统的负载的衣壳与不同的化合物iwill established. We的总体目标是一方面的基本原则在自组装过程中的（修改）球形病毒的更深入的了解。另一方面，我们想探索可能的应用，特别是那些，其中有序的金属阵列感兴趣，例如在等离子体或自旋电子学。