Active living emulsions driven by bacteria

由细菌驱动的活性活性乳液

• 批准号: 2104796
• 负责人: Jacinta Conrad
• 金额: $ 52.48万
• 依托单位: University of Houston
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2104796&HistoricalAwards=false
• 关键词: Active; living; emulsions; driven; bacteria

PART 1: NON-TECHNICAL SUMMARY:Swimming bacteria are appealing components in a novel class of “active” emulsions: suspensions of droplets whose motion is driven by bacteria. Such living active emulsions represent a potentially transformative advance in biomaterials, as they incorporate desirable properties of living systems – such as extreme mechanical properties, complex catalytic capabilities, and self-healing – into engineered materials that can be readily printed or shaped. Attaining these properties in emulsions is challenging because it is not currently known how to predict the bulk flow properties of emulsions from the motion of bacteria. This project will develop the tools needed to program the mechanical properties of living active emulsions by engineering the motility and adhesiveness of bacteria and address a critical knowledge gap by providing understanding of how the motion of external “swimmers” is transduced into collective droplet motion and “flowability” of emulsions. The results from this project are expected to advance the design of feedstocks for state-of-the-art materials processing and 3-D printing applications. The team of PIs and student trainees will disseminate results to Houston’s biomedical and materials communities through local meetings and establish a seminar series for junior Texas researchers. They will also initiate a multilingual public outreach event through the Texas Soft Matter Meeting and develop new activities for their ongoing outreach programs for K - 12 students through the University of Houston and for the public at the Houston Energy Festival.PART 2: TECHNICAL SUMMARY:The objective of this project is to develop the fundamental understanding needed to program living active emulsions in which directional rotation of suspended droplets is driven by bacterial motility. To test the hypothesis that collective interactions between bacteria-driven emulsion droplets can be used to modify and control the macroscopic mechanical properties of emulsions, the PIs will integrate complementary expertise in soft-matter physics (Conrad) and synthetic biology (Cirino) to address two specific aims: (1) control droplet mobility via bacterial interfacial interactions, and (2) link macroscopic rheology of living emulsions to microscopic collective droplet motion. The team of PIs and undergraduate and graduate student trainees will advance genetic tools for the non-model organism Marinobacter hydrocarbonoclasticus SP1 and construct genetically modified bacteria with tunable expression of surface features. Measurements of droplet motion driven by engineered bacteria will be used to test models for the rotation rate and for stochastic translational motion. The team will quantify pairwise interactions between rotating droplets, measure rotation speed and correlation length in concentrated emulsions, and relate length scales associated with collective droplet motion to the bulk mechanical properties of active emulsions. These studies will generate new insight into how the microscopic activity of bacteria is related to the macroscopic emulsion mechanics, which will pave the way for the development of living active emulsions as a new class of biomaterials.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

第1部分：非技术概要：游动的细菌是一类新型“活性”乳剂中吸引人的成分：由细菌驱动运动的液滴悬浮液。这种活活性乳剂代表了生物材料的潜在变革进步，因为它们将生命系统的理想特性（如极端的机械性能、复杂的催化能力和自我修复能力）整合到易于印刷或成型的工程材料中。在乳剂中获得这些特性是具有挑战性的，因为目前还不知道如何从细菌的运动中预测乳剂的总体流动特性。该项目将开发通过设计细菌的运动性和粘附性来编程活性乳剂的机械特性所需的工具，并通过了解外部“游泳者”的运动如何转化为集体液滴运动和乳剂的“流动性”来解决关键的知识空白。该项目的成果有望推动最先进材料加工和3d打印应用的原料设计。pi团队和学生实习生将通过当地会议向休斯顿的生物医学和材料社区传播结果，并为德克萨斯州的初级研究人员建立一系列研讨会。他们还将通过德克萨斯州软物质会议发起一项多语种的公共宣传活动，并通过休斯顿大学为K - 12学生和休斯顿能源节的公众开展新的宣传活动。第2部分：技术总结：该项目的目标是发展对活性乳剂编程所需的基本理解，其中悬浮液滴的定向旋转是由细菌运动驱动的。为了验证细菌驱动的乳化液液滴之间的集体相互作用可以用来改变和控制乳化液宏观力学性能的假设，pi将整合软物质物理学（Conrad）和合成生物学（Cirino）的互补专业知识，以解决两个具体目标：(1)通过细菌界面相互作用控制液滴的流动性；(2)将活体乳化液的宏观流变学与微观集体液滴运动联系起来。pi团队和本科生和研究生学员将推进非模式生物烃类海洋杆菌SP1的遗传工具，构建具有可调表面特征表达的转基因细菌。由工程细菌驱动的液滴运动的测量将用于测试旋转速率和随机平移运动的模型。该团队将量化旋转液滴之间的成对相互作用，测量浓缩乳剂中的旋转速度和相关长度，并将与集体液滴运动相关的长度尺度与活性乳剂的总体机械特性联系起来。这些研究将对细菌的微观活性与宏观乳化液力学之间的关系产生新的认识，为开发活性乳化液作为一类新的生物材料铺平道路。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。