RUI: Development of Novel Plasmonic Nanotransducers for Solution-based Molecular Imprinted Sensing

RUI：开发用于基于溶液的分子印迹传感的新型等离子体纳米传感器

• 批准号: 2108842
• 负责人: Ying Bao
• 金额: $ 36.65万
• 依托单位: Western Washington University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-15 至 2025-05-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2108842&HistoricalAwards=false
• 关键词: RUI; Development; Novel; Plasmonic; Nanotransducers

With the support of the Chemical Measurement and Imaging (CMI) Program in the Division of Chemistry, Dr. Ying Bao of Western Washington University is studying the development and implementation of a new class of nanoparticle-based sensors, which detect tiny amounts of biological molecules in solution. Metal nanoparticles that can be made to have waves of electrons on their surfaces by shining light on the particles are called plasmonic nanoparticles. The energy at which the electron waves are formed is dependent on the density of materials close to the surface of the plasmonic nanoparticles. Small changes in the type and density of the nearby materials cause a large change in the energy of the electron waves, that can be measured by the energy of the light absorbed by the metal nanoparticle. Such plasmonic nanoparticles will be made to detect very small numbers of protein molecules in solution by creating locations on the nanoparticles that bind to the proteins in a selective way and by placing these binding sites at locations on the nanoparticles that give the biggest change in the energy of the light that is absorbed. The selective nature of these "nanotransducers" is being achieved by making a molecular mold or imprint of proteins in a glass-like material. This molecular imprinting process creates physically robust and low-cost sensors capable of selectively binding to target molecules. This project will combine the benefits of individual plasmonic nanotransducers with molecular imprinting and open new avenues in nanosensor development. The fundamental principles that will be discovered in this research can be applied by researchers when developing the next generation of new plasmonic nanosensors, with applications in areas such as clinical diagnosis, food safety, and environmental monitoring. The project will provide opportunities for students in the Chemistry Department at Western Washington University to engage in nanomaterial-related research and gain experience in materials, environmental, and analytical chemistry. Furthermore, through both a course-based undergraduate research experience (CURE) and summer research positions, students at a local community college will gain nanoscience research experience. To develop and implement a new class of anisotropic plasmonic nanotransducers permitting solution-based molecular imprinted sensing, this project is focused on three main objectives: 1) Synthesis and fundamental study of novel plasmonic nanotransducers. 2) Performing molecular imprinting of plasmonic nanotransducers in solution. 3) Evaluating sensing properties of imprinted nanosensors in solution. Methods for site-specific deposition of silica on plasmonic nanorods, as well as methods for controlling growth of nanoscale "tentacle" features will be used and further modified to produce this novel class of anisotropic plasmonic nanotransducer. The development of such nanotransducers could address key shortcomings of existing plasmonic nanomaterials for molecular imprint-based sensing, most prominently their low stability in solution and limited effective surface area. Applying molecular imprinting and sensing in solution also has the possibility to address important restrictions in substrate-based (planar) systems, including limited numbers of imprinted molecular recognition sites, attrition in the number of sensors connected to the substrate, reduced refractive index sensitivity due to immobilization of the nanoparticles, and restricted flexibility on adjusting the number and types of nanosensors in the sensing system. The proposed fundamental studies have the potential to provide useful information for the improved design of nano-transducers and imprinted plasmonic nanosensors.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学系化学测量与成像(CMI)计划的支持下，西华盛顿大学的包英伟博士正在研究一种新型的基于纳米颗粒的传感器的开发和实施，这种传感器可以检测溶液中的微量生物分子。金属纳米粒子可以通过照射到粒子上而在其表面产生电子波，这种纳米粒子被称为等离子体纳米粒子。形成电子波的能量取决于靠近等离子体纳米粒子表面的材料的密度。附近材料的类型和密度的微小变化会导致电子波能量的巨大变化，这可以通过金属纳米颗粒吸收的光的能量来测量。这种等离子体纳米粒子将被用来检测溶液中非常少量的蛋白质分子，方法是在纳米粒子上有选择地创建与蛋白质结合的位置，并将这些结合位置放置在纳米粒子上的位置，这些位置对被吸收的光的能量产生最大的变化。这些“纳米传感器”的选择性是通过在玻璃状材料上制造蛋白质的分子模型或印记来实现的。这种分子印迹过程创造了物理上坚固和低成本的传感器，能够选择性地结合到目标分子上。该项目将把单个等离子体纳米换能器的优点与分子印迹结合起来，并为纳米传感器的开发开辟新的途径。研究人员在开发下一代等离子体纳米传感器时可以应用这项研究中发现的基本原理，这些传感器将应用于临床诊断、食品安全和环境监测等领域。该项目将为西华盛顿大学化学系的学生提供从事纳米材料相关研究的机会，并获得材料、环境和分析化学方面的经验。此外，通过基于课程的本科生研究经验(CURE)和暑期研究职位，当地社区大学的学生将获得纳米科学研究经验。为了开发和实现基于溶液的分子印迹传感的新型各向异性等离子体纳米换能器，本项目主要集中在三个方面：1)新型等离子体纳米换能器的合成和基础研究。2)在溶液中进行等离子体纳米换能器的分子印迹。3)评价印迹纳米传感器在溶液中的传感性能。在等离子体纳米棒上特定位置沉积二氧化硅的方法，以及控制纳米尺度“触角”特征生长的方法将被使用和进一步改进，以产生这种新型的各向异性等离子体纳米换能器。这种纳米换能器的发展可以解决现有等离子体纳米材料用于分子印迹传感的主要缺点，最突出的是它们在溶液中的稳定性低和有效表面积有限。在溶液中应用分子印迹和传感也有可能解决基于底物的(平面)系统中的重要限制，包括印迹分子识别位置的有限数量，连接到底物的传感器数量的磨损，由于纳米颗粒的固定而降低的折射率敏感性，以及在传感系统中调节纳米传感器的数量和类型的限制的灵活性。拟议的基础研究有可能为改进纳米换能器和印迹等离子体纳米传感器的设计提供有用的信息。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Impact on the Formation and Catalytic Property of Pt-Based Nanocatalysts by Galvanic Reaction with Co-Reduction Agents

• DOI: 10.1021/acs.chemmater.2c02659
• 发表时间: 2022-10
• 期刊: Chemistry of Materials
• 影响因子: 8.6
• 作者: John R. Crockett;Maggie Wang;Joseph E. Doebler;Tejal Pawale;Xiao Li;Ying Bao