Antimicrobial Copper - Functional Surfaces and Factors of Toxicity

通过精确地调整表面参数的地形和化学，使用激光干涉表面结构，模型表面的规模上的细菌已经产生在第一个建议。使用这些，管理润湿，离子释放和细菌粘附在金属表面上的表面参数进行了评价。此外，铜的氧化行为，在与细菌的活力测试，以及铜和银的组合，通过激光熔覆已经实施和研究on.The调整的材料和表面参数已被精确地关联到这些表面的抗菌效率。这里的重点在于材料和细菌之间的直接接触的作用，以及不同的表面电位对接触杀灭的电化学影响。正如在第一个提案中已经定量显示的那样，细菌和金属表面之间的接触显著地控制了杀灭率，在后续提案中将创建形貌定制的铜表面。在这些方面，将研究有效接触面积的变化是否以及如何影响杀灭率。在第二部份的计画中，将利用雷射干涉微影技术及连续金属镀膜技术，在表面形成具控制电位差的化学图案。通过使用这些图案化表面，通过材料组合和图案尺度的变化来研究电势差的影响。为了将其与抗菌效率相关联，将通过显微镜定量表征所研究表面上的细菌粘附，与这些研究平行。鉴于危险细菌的抗生素耐药性不断上升，以及通过使用抗菌材料对抗部分致命和高度传染性疾病，这项研究与社会特别相关。

By precisely tuning the surface parameters topography and chemistry using laser interference surface structuring, model surfaces on the scale of bacteria have been generated during the first proposal. Using these, the surface parameters that govern wetting, ion release and bacterial adhesion on metallic surfaces have been evaluated. In addition, the oxidation behavior of copper during viability tests with bacteria as well as the combination of copper and silver by laser cladding have been implemented and investigated on. The tuned material and surface parameters have been precisely correlated to the antimicrobial efficiency of these surfaces.The here described follow-up proposal is motivated by the partially surprising results from the first proposal. The emphasis here lies on the role of the immediate contact between material and bacteria as well as the electrochemical influence of differentiating surface potentials on the contact killing. As it has been quantitatively shown during the first proposal, that the contact between bacteria and metallic surface significantly governs the killing rate, topographically tailored copper surfaces will be created in the follow-up proposal. On these, it will be subject of investigation if and how the killing rate can be influenced by variation of the effective contact area. In the second part of the project, chemical surface pattern with controlled potential differences will be induced by laser interference lithography and consecutive metal coating. By using these patterned surfaces, the influence of potential differences will be studied by variation of material combination and pattern scale. In order to correlate it with antimicrobial efficiency, the bacterial adhesion on the investigated surfaces will be quantitatively characterized by microscope, parallel to these studies. This research is specifically relevant for the society in the light of rising antibiotics resistance of dangerous germs and the fight of partly deadly and highly infectious diseases by employing antimicrobial materials.