Responsiveness of sex determination to thermal cues and maternal estrogens as potential modifiers

性别决定对热线索和母体雌激素作为潜在调节剂的反应

基本信息

项目摘要

Most organisms respond to environmental temperatures, and these responses are typically studied by comparing individuals held at one temperature to individuals held at a different temperature. This approach misses two important points: 1) temperatures in nature are often variable and exposure to a given temperature can be transient, and 2) it does a poor job quantifying variation in how long it takes individuals to respond to temperature. This project focuses on understanding why some individuals respond to a short exposure to a given temperature, while others do not. Studies will be conducted using the red-eared slider turtle (Trachemys scripta), where incubation temperatures affect sex determination, which provides a clear signal (i.e., formation of a testis or ovary) of how individuals responded to temperature. Specifically, cool incubation temperatures induce testis development and warm incubation temperatures induce ovary development. It is also established that higher levels of estrogens in the egg increase the likelihood that the embryos will develop ovaries. Under natural conditions, embryos routinely experience both testis and ovary inducing temperatures, so studies are aimed at deciphering how long of an exposure to warmer temperatures is necessary to induce ovary development and whether elevated estrogen levels reduce the exposure requirement. Advancing our understanding of how temperature affects sex determination will assist efforts to mitigate the effect of climate change on thermally sensitive species. The project will train a postdoctoral researcher, graduate and undergraduate students, and will engage pre-college students in STEM-related activities. Recent studies have demonstrated that temperature-dependent sex determination involves epigenetic regulation of critical transcription factors that determine sex. In the red-eared slider turtle (Trachemys scripta), the histone demethylase Kdm6b removes repressive histones marks from the promoter of Dmrt1, which is a conserved regulator of testis development. For this species, testis development is induced at cool incubation temperatures (below 29˚C). This proposal examines how sex determination occurs under ecologically relevant conditions where incubation temperatures fluctuate between those that induce either testis production or ovary production. Projects will focus on the temporal aspect of the response to temperatures since it is established that exposure to specific incubation temperatures is transient in nature and individual embryos vary in whether their gonadal fate is influenced by brief exposures (e.g., heatwaves). Additionally, projects will investigate whether maternal estrogens affect the duration of exposure necessary to induce ovarian development. By characterizing how the expression of genes involved in gonadal differentiation respond to transient exposure to incubation temperature and how estrogens my influence that response, studies will begin to disentangle how molecular responses to warm and cool temperature may vary independently. Understanding how an organism responds to thermal variation will strengthen our understanding of how environmental temperatures regulate biological processes such as sex determination.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
大多数生物体对环境温度有反应,这些反应通常通过比较保持在一个温度下的个体与保持在不同温度下的个体来研究。这种方法忽略了两个重要的点:1)自然界中的温度通常是可变的,暴露于给定的温度可能是短暂的,2)它在量化个体对温度做出反应所需时间的变化方面做得很差。这个项目的重点是了解为什么有些人对短时间暴露在给定的温度下有反应,而另一些人则没有。将使用红耳滑龟(Trachemys scripta)进行研究,其中孵育温度影响性别决定,这提供了明确的信号(即,睾丸或卵巢的形成)了解个体对温度的反应。具体地说,冷孵育温度诱导睾丸发育和温暖的孵育温度诱导卵巢发育。它还确定,卵子中较高水平的雌激素增加了胚胎发育卵巢的可能性。在自然条件下,胚胎通常会经历睾丸和卵巢诱导温度,因此研究的目的是破译暴露于更温暖的温度多久才能诱导卵巢发育,以及雌激素水平升高是否会降低暴露要求。推进我们对温度如何影响性别决定的理解将有助于减轻气候变化对热敏感物种的影响。该项目将培训一名博士后研究员、研究生和本科生,并将让大学预科生参与STEM相关活动。最近的研究表明,温度依赖性性别决定涉及决定性别的关键转录因子的表观遗传调控。在红耳滑龟(Trachemys scripta)中,组蛋白去甲基化酶Kdm6b从Dmrt 1的启动子中去除抑制性组蛋白标记,Dmrt 1是睾丸发育的保守调节因子。对于该物种,睾丸发育在低温孵育温度(低于29 ℃)下诱导。这个建议探讨如何性别决定发生在生态相关的条件下,孵化温度之间波动,诱导睾丸生产或卵巢生产。项目将侧重于对温度反应的时间方面,因为已经确定,暴露于特定的孵育温度在性质上是短暂的,单个胚胎的性腺命运是否受到短暂暴露的影响(例如,热浪)。此外,项目将调查母体雌激素是否影响诱导卵巢发育所需的暴露时间。通过描述性腺分化相关基因的表达对短暂暴露于孵育温度的反应以及雌激素如何影响这种反应,研究将开始解开分子对温暖和凉爽温度的反应如何独立变化。了解生物体对温度变化的反应将加强我们对环境温度如何调节生物过程的理解,例如性别决定。该奖项反映了NSF的法定使命,并通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。

项目成果

期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Temperature fluctuations and estrone sulfate affect gene expression via different mechanisms to promote female development in a species with temperature-dependent sex determination
温度波动和硫酸雌酮通过不同机制影响基因表达,以促进具有温度依赖性性别决定的物种中的雌性发育
  • DOI:
    10.1242/jeb.244211
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    2.8
  • 作者:
    Marroquín-Flores, Rosario A.;Paitz, Ryan T.;Bowden, Rachel M.
  • 通讯作者:
    Bowden, Rachel M.
Understanding how variable thermal environments affect the molecular mechanisms underlying temperature-sensitive phenotypes: lessons from sex determination
了解可变的热环境如何影响温度敏感表型的分子机制:性别决定的教训
  • DOI:
    10.1242/jeb.242373
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    2.8
  • 作者:
    Paitz, Ryan T.;Breitenbach, Anthony T.;Marroquín-Flores, Rosario A.;Bowden, Rachel M.
  • 通讯作者:
    Bowden, Rachel M.
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Rachel Bowden其他文献

Teacher Research in the English Language Teacher Development Project
英语教师发展项目中的教师研究
  • DOI:
    10.1057/9781137376220_12
  • 发表时间:
    2015
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Rachel Bowden
  • 通讯作者:
    Rachel Bowden
Fantasy Higher Education: University and college league tables
  • DOI:
    10.1080/13538320050001063
  • 发表时间:
    2000-04
  • 期刊:
  • 影响因子:
    1.4
  • 作者:
    Rachel Bowden
  • 通讯作者:
    Rachel Bowden
Professional Doctorates in England
英国专业博士学位
  • DOI:
    10.1080/03075070124819
  • 发表时间:
    2001
  • 期刊:
  • 影响因子:
    0
  • 作者:
    T. Bourner;Rachel Bowden;Stuart Laing
  • 通讯作者:
    Stuart Laing
Learner-centred education and English medium instruction: policies in practice in a lower-secondary mathematics class in rural Rwanda
以学习者为中心的教育和英语授课:卢旺达农村初中数学课堂的实践政策
  • DOI:
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    1.8
  • 作者:
    Rachel Bowden;Innocente Uwineza;J. Dushimimana;Alphonse Uworwabayeho
  • 通讯作者:
    Alphonse Uworwabayeho
Early years postgraduate surgical training programmes in the UK are failing to meet national quality standards: An analysis from the ASiT/BOTA Lost Tribe prospective cohort study of 2,569 surgical trainees.
英国早期的研究生外科培训项目未能达到国家质量标准:ASiT/BOTA Lost Tribe 前瞻性队列研究对 2,569 名外科学员进行的分析。
  • DOI:
    10.1016/j.ijsu.2017.09.074
  • 发表时间:
    2018
  • 期刊:
  • 影响因子:
    15.3
  • 作者:
    J. Glasbey;R. Harries;A. Beamish;V. Gokani;H. Mohan;Adam P Williams;Simon Fleming;Aaron Chai;Abhinav Singh;A. Stoneham;A. Lunt;Adeeb H. Rehman;A. Dhahri;A. Yvon;A. Dutta;A. Abou;A. Abdelrahman;A. Daoub;A. Sanalla;A. D. G. Rico;Alastair Konarski;A. Ward;Alexis Wilkin;Alexandra K. Winter;A. Arnaout;A. Bakhsh;A. Esfandiari;Alistair W. Hardy;A. Khan;A. Thacoor;A. Gavrila;Anca;Andreas Fontalis;A. Hall;A. Williamson;A. Kosti;A. Harlinska;A. Adimonye;A. Egglestone;A. Thaventhiran;A. Myatt;A. Vusirikala;A. Rawashdeh;Arjun C. Paramasivan;A. Cotton;A. Scrimshire;A. Ramesh;A. Krishnamoorthy;Asif Ahmed;Ayeshah Abdul;Ayushah Khan;B. Oremule;B. Ho;B. Barkham;B. Collard;B. Edgar;B. Drake;Bethan John;C. R. Gordon;C. Rossborough;Chang Park;C. Seretis;C. H. Johnson;C. Gill;C. Serino;C. Ogbuokiri;C. Swords;C. Kang;Chris McKinnon;Christopher E. Brown;C. J. Manning;C. Marusza;Christopher P Jones;C. Forde;Claire L. Wilson;C. Koh;Conal Horgan;Daniel J. Lin;D. Ashmore;D. Ness;Daniel O. Akhtar;D. Doherty;D. Scholfield;D. Ensor;D. Bratt;David J. Spence;D. Thomson;D. Ferguson;D. Apparau;Devaraj M. Navaratnam;D. Mai;D. Rutherford;E. Karam;E. Wu;E. Zimmermann;E. Douka;E. Flatt;E. Kane;Elizabeth L. Thornhill;E. Gammeri;E. Littlehales;E. M. Valsamis;E. Hankin;E. Meenan;Etienne Botha;F. Khalid;Fatema Patel;F. Power;Fiona Rutherford;Fozia Saeed;Francesca L. Guest;Francisco J. Barbosa;F. Cameron;Furqan R. Raja;G. Thiruchandran;G. Munbauhal;G. Dovey;G. E. Hogg;G. Dovell;G. Matheron;G. T. Hill;G. Layton;Georgiana G.S. Jong;G. Hicks;G. Millward;Grahame Shaw;G. Stamp;H. Parwaiz;H. H. Chong;H. Copley;Hannah Lennox;Hannah M. Emerson;H. F. Dean;H. Eltyeb;H. Chu;I. Sadien;Imran Mohamed;I. Parwaiz;Isabella Drummond;J. Pearce;J. Ahmed;J. Koris;J. Rait;James Bailey;J. A. Cohen;James A. Kennedy;J. Olivier;James Bailey;James E. Archer;James J. Stewart;James Barros;James R. Allen;J. W. O'Brien;James;T. McGhee;Jared Quarcoopome;J. Winyard;J. Roberts;J. Barwell;Jeremy Rodrigues;J. Chapman;J. Fairbanks;J. Voll;J. Lim;Jinlai Chang;J. Bovis;J. Ferns;J. Tam;J. Herron;J. Macdonald;J. Ducey;Joseph W. Dixon;Joshua T. Luck;K. Hewage;K. Yakoub;K. Bhopal;K. Vejsbjerg;K. Aboelmagd;K. Bera;K. Hamlett;Katherine E. Fok;K. Hurst;K. Gillams;Katie L. Siggens;K. Young;Ken Burns;K. Burke;K. Seebah;Khalid Shah;Kieran Bentick;Kiran Majid;Kirsty L. Davies;K;K. Baryeh;L. Phillips;L. Ellerton;Leeying Giet;L. Monaghan;L. Cheung;Louise L. Shen;L. Paramore;L. Arrowsmith;L. Attwell;Luke Thornton;L. Xu;M. Leadon;M. Natarajan;M. Houlihan;Marisa Cheah;M. Sagmeister;Maryam O Abubakar;Matthew F. Flynn;M. Harris;M. Stone;M. Young;M. Gray;M. Horner;M. Schembri;M. Trail;M. Joy;M. Rice;Michael P. Thomas;M. Poon;M. Stoddart;M. L. Fong;Mitchell T. Foster;M. Mohamud;M. N. Hoque;M. Remtulla;Mohsin Javed Karim;M. Řezáčová;M. B. Siddiqui;M. Iqbal;M. Mensa;N. McCauley;N. Bauer;N. Walker;N. A. Hakim;Ngonidzashe Knight;N. O'Hara;Nicholas A. Fawcett;N. Wong;Nicola F.D. Allen;N. Husnoo;N. Vallabh;N. Srikandarajah;Nnaemeka Chidumije;O. Elamin;Odunayo Akinlaja;O. Griffiths;Oliver D Brown;O. Shastri;Olivia J. Cameron;Olivia Kenyon;O. Javed;O. Sogaolu;P. Birmpili;P. Haylock;P. Green;P. Carroll;Peiming Yang;P. Beak;Pia Persson;P. J. Tam;Rabia Waqar;R. Morley;Rachel Bowden;Rachel L. Eyre;R. Pankhania;R. Sahemey;R. Kabariti;M. Rawashdeh;Rawashdeh S. Arab;R. Rollett;R. Nicholas;R. Morgan;R. Limb;R. Robinson;Richard S Hayes;R. Daureeawoo;R. A. Cooke;R. Espey;R. Chessman;R. Whitham;R. E. Payne;R. Staruch;R. Alho;R. Gordon;R. Cuthbert;Roseanna B. Harrison;R. Scott;R. M. Parks;R. Cheong;Ryan Hillier;Ryan Moffatt;Saad Rehman;Sabah Ambren;S. Abdulal;S. Kulkarni;S. Hopwood;S. Greenfield;S. Mehta;S. Haines;S. Alsaati;S. Williams;S. El;S. Barlow;S. Pywell;S. Pollock;S. Lampridis;S. Nazarian;S. Rezvani;S. Scattergood;S. Toescu;S. Hotonu;S. Shaikh;S. Rupani;Shumaila Hasan;S. Pradeep;S. Cole;S. Growcott;Sofia E. Bedoya;S. Ike;S. Bodnarescu;S. C. Seppings;S. A. Poyntz;S. Jordan;Sundas Iqbal;Suparna Das;S. Chatterjee;S. Shumon;Tamsin E. M. Morrison;T. Sibartie;T. Aboelmagd;Thomas B. Russell;Thomas Seddon;T. Stringfellow;T. Goldsmith;Thomas H. Banks;T. Tolley;T. Oputa;T. Kanzara;T. Challoner;T. Urbonas;T. Richards;V. Morrison;Vivek Garikapati;Waheeb Al;Wajiha Zahra;W. Ho;W. Al;W. Gibson;Yasmin Grant;Y. Hijazi;Yayganeh Chiang;Z. Gundkalli;Zoe M. Seymour;Z. Panayi
  • 通讯作者:
    Z. Panayi

Rachel Bowden的其他文献

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{{ truncateString('Rachel Bowden', 18)}}的其他基金

Embryonic regulation of the endocrine environment via the sulfotransferase-sulfatase pathway in an oviparous vertebrate
卵生脊椎动物通过磺基转移酶-硫酸酯酶途径对内分泌环境的胚胎调节
  • 批准号:
    0952840
  • 财政年份:
    2010
  • 资助金额:
    $ 89.55万
  • 项目类别:
    Continuing Grant
Endocrine-Immune Interactions: Estrogens as Immunomodulators
内分泌免疫相互作用:雌激素作为免疫调节剂
  • 批准号:
    0748505
  • 财政年份:
    2008
  • 资助金额:
    $ 89.55万
  • 项目类别:
    Standard Grant
CONFERENCE- CONSEQUENCES OF MATERNALLY DERIVED YOLK HORMONES FOR OFFSPRING PHENOTYPE: CURRENT STATUS, CHALLENGES, AND OPPORTUNITIES, TO BE HELD JANUARY 2-6,2008 IN SAN ANTONIO, TX.
会议——母体卵黄激素对后代表型的影响:现状、挑战和机遇,将于 2008 年 1 月 2-6 日在德克萨斯州圣安东尼奥举行。
  • 批准号:
    0737608
  • 财政年份:
    2007
  • 资助金额:
    $ 89.55万
  • 项目类别:
    Standard Grant

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