PlantSynBio: Chassis design for sustainable production of high value terpenoids in the crop species tomato
PlantSynBio:用于在番茄作物中可持续生产高价值萜类化合物的底盘设计
基本信息
- 批准号:2126592
- 负责人:
- 金额:$ 140万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-10-01 至 2025-09-30
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Plants produce a diverse set of chemicals that are of use to humans as pharmaceuticals, fragrance, food additives, and agrichemicals. One class of compounds are terpenoids which can be used as agrichemicals as they are active against many insects but non-toxic to mammals, biodegradable, and compatible with methods used in sustainable agriculture practices. However, production of these compounds directly from plants can be challenging due to low yield as well as the time, space, and costs required to grow the plants and extract the compounds. Through advances in biotechnology, it is now possible to engineer production of high value chemicals in plants in a crop species which is simple to grow thereby providing a sustainable approach to production of plant-derived agrichemicals. In this project, we will use biotechnology approaches to engineer tomato to produce high value terpenoids in fruit and demonstrate that merging chemistry with biotechnology and agriculture provides a platform for sustainable production of chemicals with limited availability from natural sources. We anticipate that access to a sustainable bio-engineered platform for terpenoid production will be of broad use to both the academic and industry sectors. In this project, we will train personnel in technologies critical to a 21st century workforce. Through annual events and modules at the State Botanic Garden of Georgia, Michigan State University, and University of Minnesota, we will inform the public of the potential of plant systems for sustainable production of chemicals.Over 50,000 plant terpenoids are known which are synthesized from universal C5 building blocks via the activity of terpene synthases, cytochrome P450s, and glycosyl transferases and function in plant defense, and as attractants to pollinators and symbionts. However, heterologous expression of terpenoid biosynthetic genes in plants is problematic due to the tendency for conversion of the activated products into conjugates or other derivatives via endogenous cytochrome P450s and glycosyl transferases. Using cutting edge gene editing and transformation technologies, this project will develop new experimental platforms and a paradigm for plant synthetic biology by generating two novel tomato chassis with minimal terpenoid biosynthetic capacity that will enable engineering of high value terpenoid molecules in tomato fruit. Our scientific aims are to (i) Rapidly confirm cytochromes P450 and glycosyl transferases involved in tri- and tetra-terpene biosynthesis in tomato fruit using de novo meristem generated gene-edited lines; (ii) Develop two chassis in tomato that are depleted of tri- and tetraterpenes (carotenoids) in fruit using state-of-the-art gene editing and transformation methods; and (iii) Construct bioengineered tomato lines that synthesize two high value terpenoids as a proof-of-concept. Within this project we will train scientists in state-of-the-art synthetic biology to facilitate new paradigms in plant synthetic biology. Through annual events and modules with three institutions, we will engage the lay public in the power of plant systems for sustainable chemical production.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
植物产生一系列对人类有用的化学物质,如药品、香料、食品添加剂和农用化学品。一类化合物是萜类化合物,可用作农用化学品,因为它们对许多昆虫有活性,但对哺乳动物无毒,可生物降解,并与可持续农业实践中使用的方法兼容。然而,直接从植物中生产这些化合物可能具有挑战性,因为产量低,以及种植植物和提取化合物所需的时间、空间和成本。通过生物技术的进步,现在有可能在一种易于种植的作物品种中设计生产高价值化学品的植物,从而提供一种可持续的方法来生产植物衍生的农用化学品。在这个项目中,我们将使用生物技术方法来改造番茄,使其在水果中生产高价值的萜类化合物,并证明将化学与生物技术和农业相结合,为可持续生产自然来源有限的化学品提供了一个平台。我们预计,获得用于萜类化合物生产的可持续生物工程平台将对学术界和工业界都有广泛的用途。在这个项目中,我们将培训对21世纪劳动力至关重要的技术人员。通过佐治亚州州立植物园、密歇根州立大学和明尼苏达大学的年度活动和模块,我们将向公众介绍植物系统可持续生产化学物质的潜力。已知的50,000多种植物萜类化合物是从通用的C5积木中通过萜烯合成酶、细胞色素P450和糖基转移酶的活性合成的,具有植物防御功能,并作为传粉者和共生体的引诱剂。然而,萜类生物合成基因在植物中的异源表达是有问题的,因为激活的产物往往通过内源细胞色素P450和糖基转移酶转化为偶联物或其他衍生物。利用尖端的基因编辑和转化技术,该项目将开发新的实验平台和植物合成生物学的范例,通过产生两个具有最低萜类生物合成能力的新型番茄底盘,使番茄果实中的高价值萜类分子得以工程。我们的科学目标是:(I)利用新生分生组织产生的基因编辑系,快速确认番茄果实中参与三萜和四萜生物合成的细胞色素P450和糖基转移酶;(Ii)利用最先进的基因编辑和转化方法,开发两个去除果实中三萜和四萜(类胡萝卜素)的番茄底盘;以及(Iii)构建合成两个高价值萜类化合物的生物工程番茄品系。在这个项目中,我们将培训最先进的合成生物学的科学家,以促进植物合成生物学的新范例。通过与三个机构的年度活动和模块,我们将让普通公众参与可持续化学生产的工厂系统的力量。该奖项反映了NSF的法定使命,并通过使用基金会的智力优势和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Carol Buell其他文献
Carol Buell的其他文献
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{{ truncateString('Carol Buell', 18)}}的其他基金
PlantSynBio: Construction of a plant chassis as a platform for biological discoveries and innovations
PlantSynBio:构建植物底盘作为生物发现和创新的平台
- 批准号:
2308873 - 财政年份:2023
- 资助金额:
$ 140万 - 项目类别:
Standard Grant
EAGER: Comparative single cell transcriptomics and regulomics: A proof-of-concept application of cutting-edge -omics techniques with non-model systems
EAGER:比较单细胞转录组学和调节组学:尖端组学技术与非模型系统的概念验证应用
- 批准号:
2309665 - 财政年份:2023
- 资助金额:
$ 140万 - 项目类别:
Standard Grant
RESEARCH-PGR: Multiple origins of tuber formation: Evolution of a unique storage organ
研究-PGR:块茎形成的多重起源:独特储存器官的进化
- 批准号:
2140176 - 财政年份:2021
- 资助金额:
$ 140万 - 项目类别:
Standard Grant
RESEARCH-PGR: Multiple origins of tuber formation: Evolution of a unique storage organ
研究-PGR:块茎形成的多重起源:独特储存器官的进化
- 批准号:
1929982 - 财政年份:2019
- 资助金额:
$ 140万 - 项目类别:
Standard Grant
Evolution of Specialized Metabolite Biosynthetic Pathways in the Lamiaceae: Sources of Chemical Diversity for Molecules Essential for Human Use and Plant Defense
唇形科专门代谢物生物合成途径的进化:人类使用和植物防御所必需的分子化学多样性的来源
- 批准号:
1444499 - 财政年份:2015
- 资助金额:
$ 140万 - 项目类别:
Continuing Grant
Unraveling the Heterozygosity, Allelic Composition, and Copy Number Variation of Potato
揭示马铃薯的杂合性、等位基因组成和拷贝数变异
- 批准号:
1237969 - 财政年份:2012
- 资助金额:
$ 140万 - 项目类别:
Continuing Grant
U.S.-Peru Workshop: International Collaborations in Cyberinfrastructure-enabled Genomics Workshop
美国-秘鲁研讨会:网络基础设施支持的基因组学研讨会的国际合作
- 批准号:
0742699 - 财政年份:2008
- 资助金额:
$ 140万 - 项目类别:
Standard Grant
Generation of Potato Sequence and Annotation Resources
马铃薯序列和注释资源的生成
- 批准号:
0834044 - 财政年份:2007
- 资助金额:
$ 140万 - 项目类别:
Continuing Grant
An Annotation Resource for the Rice Genome
水稻基因组注释资源
- 批准号:
0834043 - 财政年份:2007
- 资助金额:
$ 140万 - 项目类别:
Continuing Grant
Generation of Potato Sequence and Annotation Resources
马铃薯序列和注释资源的生成
- 批准号:
0604907 - 财政年份:2006
- 资助金额:
$ 140万 - 项目类别:
Continuing Grant
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