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Collaborative Research: Deciphering the molecular mechanisms of hormone-like function of terpenoids

合作研究：破译萜类激素类激素功能的分子机制

基本信息

批准号：
2139804
负责人：
Natalia Doudareva
金额：
$ 78.53万
依托单位：
Purdue University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2022
资助国家：
美国
起止时间：
2022-08-01 至 2025-07-31
项目状态：
未结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2139804&HistoricalAwards=false
关键词：
Collaborative Research Deciphering molecular mechanisms

项目摘要

Plants emit an amazing diversity of volatile organic compounds (VOCs) that play numerous roles in plant growth and development. They are also targets of released compounds as a part of plant-plant communication, as well as plant-insect and plant-microbe interactions. Plants are constantly exposed to atmospheric VOCs and can differentiate and respond to specific cues, therefore VOC perception is an essential part of information exchange. Due to the plethora of biological processes dependent on VOCs, significant progress has been made towards understanding the biosynthesis of plant VOCs and their regulation, and, in recent years, the molecular mechanisms involved in VOC emission. However, to date little is known about how VOCs are perceived by plants and trigger cellular response(s). This project will characterize signaling pathway(s) involved in perception of volatiles and mode of action of volatile signals, particularly terpenoids, in plant growth and development. This research will also answer the long-standing question about the nature of unknown endogenous plant signaling molecule(s). The results will fill important gaps in our understanding of plant olfaction and elucidate the molecular mechanisms underlying volatile perception and responses in plants. The obtained information will be groundbreaking for our understanding of plant interactions in the ecosystem and uncover the differences between volatile perception in plants and that in animals and insects. Given the VOC multifunctionality and overall lack of understanding of volatile perception in plants, this research will identify new targets for metabolic engineering for simultaneous improvement of plant defense and fitness. The proposed research will provide multidisciplinary training to undergraduate and graduate students, and postdoctoral researchers. The educational program will also introduce STEM-based research to a local middle school with minority students. The goals are to improve student enthusiasm, interest, and perceptions about scientific careers.Perception is an essential part of plant-plant, plant-insect and plant-microbe interactions, but the molecular mechanisms remain unknown. The absence of reliable molecular markers of the perception state makes the investigation of plant communication difficult. The recent discovery in petunia flowers of inter-organ aerial transport of volatiles via natural fumigation and hormone-like function of terpenoids, provides an excellent platform for investigating the mode of VOC perception and dissecting signaling cascade(s) involved. Preliminary results in Petunia hybrida flowers led to hypothesis that a karrikin-like signaling pathway is involved in perception of volatile signals via a karrikin-insensitive receptor, PhKAI2ia, and mediates terpene-dependent stigma development. This research will employ an integrative strategy comprised of genetics, molecular biology, biochemistry, metabolic profiling, structural and cell biology to (1) identify the targets of the new KAI2ia-dependent volatile sesquiterpene signaling pathway; (2) perform structure-functional analysis of PhKAI2ia receptor and elucidate the molecular features that determine its substrate specificity/selectivity; and (3) determine whether the KAI2ia-dependent sesquiterpene signalling pathway operates via MAX2 ubiquitin ligase essential for karrikin signaling. This work will uncover yet unknown role(s) of KAI2is in plant VOC perception, illuminate KAI2ia-dependent signaling pathway from organismal to molecular levels and answer the question of how MAX2 ubiquitin ligases deliberate ligand-specific responses.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

植物释放出多种多样的挥发性有机化合物（VOCs），这些化合物在植物的生长和发育中发挥着许多作用。它们也是释放的化合物的目标，作为植物-植物通信的一部分，以及植物-昆虫和植物-微生物相互作用。植物不断暴露于大气中的VOC，并且可以区分和响应特定的线索，因此VOC感知是信息交换的重要组成部分。由于大量的生物过程依赖于挥发性有机化合物，在理解植物挥发性有机化合物的生物合成及其调控方面取得了重大进展，近年来，涉及挥发性有机化合物排放的分子机制也取得了重大进展。然而，迄今为止，人们对植物如何感知VOC并触发细胞反应知之甚少。该项目将表征参与挥发物感知的信号通路和挥发物信号的作用模式，特别是萜类化合物，在植物生长和发育中。这项研究还将回答长期存在的关于未知内源植物信号分子性质的问题。这些结果将填补我们对植物嗅觉理解的重要空白，并阐明植物挥发性感知和反应的分子机制。所获得的信息将是我们对生态系统中植物相互作用的理解的突破，并揭示植物与动物和昆虫之间挥发性感知的差异。鉴于挥发性有机化合物的多功能性和对植物挥发性感知的整体缺乏了解，这项研究将确定代谢工程的新目标，以同时改善植物的防御和适应性。拟议的研究将为本科生和研究生以及博士后研究人员提供多学科培训。该教育项目还将向当地一所有少数民族学生的中学介绍基于STEM的研究。感知是植物-植物、植物-昆虫和植物-微生物相互作用的重要组成部分，但其分子机制尚不清楚。缺乏可靠的分子标记的感知状态，使植物通讯的调查困难。最近在矮牵牛花中发现的挥发物通过天然熏蒸和萜类化合物的类似于烟草的功能在器官间空中运输，为研究VOC感知模式和解剖所涉及的信号级联提供了一个很好的平台。在矮牵牛花的初步结果导致的假设，karrikin样信号通路参与感知挥发性信号通过karrikin不敏感的受体，PhKAI 2 ia，并介导terrandom-dependent柱头发育。本研究将采用遗传学、分子生物学、生物化学、代谢谱、结构生物学和细胞生物学相结合的研究策略，（1）确定新的KAI 2 ia依赖性挥发性倍半萜信号通路的靶点;（2）对PhKAI 2 ia受体进行结构-功能分析，阐明决定其底物特异性/选择性的分子特征;和（3）确定KAI 2 ia依赖性倍半萜信号传导途径是否通过karrikin信号传导所必需的MAX2泛素连接酶起作用。这项工作将揭示KAI 2 is在植物VOC感知中的未知作用，阐明从生物体到分子水平的KAI 2 ia依赖性信号传导途径，并回答MAX2泛素如何连接故意配体特异性反应的问题。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。