Deciphering the Complex Metabolic Network in Snapdragon Flowers: An Integrative Approach

破译金鱼草花中复杂的代谢网络：一种综合方法

• 批准号: 0615700
• 负责人: Natalia Doudareva
• 金额: $ 103万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0615700&HistoricalAwards=false
• 关键词: Deciphering; Complex; Metabolic; Network; Snapdragon

Recent progress in the field of secondary metabolism has uncovered the importance of secondary metabolites, not only for plant life, but also for humans. The involvement of secondary metabolites in plant survival in the surrounding ecosystem and their impact on food quality, attractiveness of ornamentals, and human health reveal the importance of genetic improvements leading to their formation. Genetic engineering of secondary metabolites has enormous potential. However, its success largely depends on our understanding of the entire cellular metabolic network. The long-term goal of this project is to provide a comprehensive understanding of in vivo metabolism and to characterize the carbon flow through the metabolic network in photosynthetic and non-photosynthetic floral tissues over the lifespan of the flower. A unique integrative approach, including transient stable isotope labeling and metabolic flux analysis over flower development, combined with corresponding transcriptome analysis, will be used to obtain a novel picture of the dynamic relationships between in vivo flux and gene expression profiles in central carbon, nitrogen, and secondary metabolism of flowers. This research will lead to the generation of a temporal flux map in which each biochemical step will contain a developmental profile for the corresponding flux and gene expression. The developmental aspect of flux analysis incorporated into the temporal flux map will add a new dimension to our vision of primary and secondary metabolism. The integration of global flux analysis and enzyme activity with gene expression will uncover the potential regulatory steps within pathways or branchways. An integrative map developed in this research will help to conceptualize the temporal and spatial aspects of the network regulation and guide diverse efforts in metabolic engineering of plant secondary metabolism. Moreover, this project will fill important gaps in the understanding of floral metabolism and provide powerful modeling tools for general use in the metabolic engineering of plant metabolic pathways. Broader impacts: This project has a strong multidisciplinary training component for the next generation of plant scientists. Undergraduate and graduate students, as well as post-doctoral scientists (including women and minorities) participating in this research will get 'the big picture' of cellular metabolism and gain an appreciation of whole plant physiology, in vivo isotopic labeling, analytical biochemistry, molecular biology, genetics and integrative modeling. The overall instructional goal is to provide students with a more comprehensive understanding of plant metabolism. In addition to training students, new course modules on secondary metabolism, metabolic engineering, and integrative modeling will be developed and incorporated via the web using WebCT into existing courses at Purdue University. Plant metabolic modeling software developed as part of this project as well as the teaching modules will be released as freeware on the Purdue Horticulture web server.

近年来在植物次生代谢领域的研究进展揭示了植物次生代谢物的重要性，不仅对植物，而且对人类也是如此。次生代谢产物参与植物在周围生态系统中的生存及其对食品质量、植物的吸引力和人类健康的影响，揭示了导致其形成的遗传改良的重要性。次生代谢物的基因工程具有巨大的潜力。然而，它的成功在很大程度上取决于我们对整个细胞代谢网络的理解。该项目的长期目标是提供对体内代谢的全面了解，并表征在花的生命周期内通过光合和非光合花组织中代谢网络的碳流。一个独特的综合方法，包括瞬时稳定同位素标记和代谢通量分析花发育，结合相应的转录组分析，将被用来获得一个新的图片在体内通量和基因表达谱的中心碳，氮，和次生代谢的花之间的动态关系。这项研究将导致产生一个时间通量图，其中每个生化步骤将包含相应的通量和基因表达的发育概况。纳入时间通量图的通量分析的发展方面将为我们对初级和次级代谢的愿景增添新的维度。全局通量分析和酶活性与基因表达的整合将揭示通路或分支中潜在的调控步骤。 在这项研究中开发的综合地图将有助于概念化的网络调控的时间和空间方面，并指导植物次生代谢的代谢工程的各种努力。此外，该项目将填补重要的空白，了解花代谢，并提供强大的建模工具，一般用于植物代谢途径的代谢工程。更广泛的影响：该项目为下一代植物科学家提供了强有力的多学科培训。参与这项研究的本科生和研究生以及博士后科学家（包括女性和少数民族）将获得细胞代谢的“大局”，并获得整个植物生理学，体内同位素标记，分析生物化学，分子生物学，遗传学和综合建模的欣赏。总的教学目标是让学生对植物代谢有更全面的了解。除了培训学生，新的课程模块，次级代谢，代谢工程和综合建模将开发并通过网络使用WebCT纳入普渡大学现有的课程。作为该项目的一部分开发的植物代谢建模软件以及教学模块将作为免费软件在普渡园艺网络服务器上发布。