I-Corps: Multimodal imaging platform to image bioenergetics in live tissue

这个i-Corps项目的更广泛的影响/商业潜力是开发一种高分辨率反射成像平台，可以测量组织中的氧气水平。建议的平台可能会集成到其他成像平台中。目前市场上可用的成像平台从相同的视野提供关于组织氧合和新陈代谢的信息。这两个指标可能提供有关组织状态的关键信息，这可能有助于癌症、缺血再灌注损伤、阿尔茨海默病和阻塞性睡眠呼吸暂停等疾病的治疗和管理。开发针对这些疾病的更好的治疗方法需要对体内组织生物能量学有更好的了解。最终，这种成像平台的成功开发和商业化可以为组织提供一个新的视角，有助于改善疾病的治疗和管理。该i-Corps项目是基于开发一种高分辨率、高光谱暗场反射成像平台，该平台可以集成到其他成像模式中，以提供来自同一视场的多模式时空信息。这项研究的最初重点是将该平台集成到一个双光子显微镜中，以提供对两个关键组织水平指标-微血管氧合和细胞代谢-的定量测量。越来越多的证据表明，只测量这些指标中的一个并不能提供关于另一个的直接信息，并且测量血管氧合和细胞新陈代谢之间的时空关系对于确定病变组织的长期结果非常重要。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

The broader impact/commercial potential of this I-Corps project is the development of a high-resolution reflectance imaging platform that can measure the oxygen levels in tissues. The proposed platform that may be integrated into other imaging platforms. The imaging platforms that are currently available in the market provide information about tissue oxygenation and metabolism from the same field of view. These two metrics may provide critical information about the state of tissue, which may aid in the treatment and management of diseases such as cancer, ischemia-reperfusion injury, Alzheimer’s disease, and obstructive sleep apnea. Developing better therapeutics to target these diseases demands a better understanding of tissue bioenergetics in vivo. Ultimately, successful development and commercialization of this imaging platform could provide a new look at tissue that helps improve treatment and management of disease. This I-Corps project is based on the development of a high-resolution, hyper-spectral dark field reflectance imaging platform that may be integrated into other modes of imaging to provide multi-modal spatiotemporal information from the same field of view. The initial focus of this research is to integrate the platform into a two-photon microscope to provide quantitative measures of two critical tissue-level metrics - microvascular oxygenation and cellular metabolism. There is increasing evidence that measurement of only one of these metrics does not provide direct information about the other and that measuring the spatiotemporal relationship between vascular oxygenation and cellular metabolism is important for determining long-term outcomes in diseased tissue.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.