SpiderMAEN: Recombinant Spider Silk-based Hybrid Materials for Advanced Energy Technology

SpiderMAEN：用于先进能源技术的重组蜘蛛丝混合材料

• 批准号: 250591669
• 负责人: Professor Dr. Thomas Scheibel
• 金额: --
• 依托单位: Lehrstuhl für Supramolekulare Chemie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/250591669?language=en
• 关键词: SpiderMAEN; Recombinant; Spider; Silk; based

The project proposed here focuses on the synthesis of recombinant proteins based on spider silk. Specifically, peptide sequences known to bind to inorganic materials such as titania, silver, and gold shall be incorporated into the spider silk. Electrospinning will yield fibers and fiber mats to be used as templates / matrices for mineralization of the said inorganics. The main goals of the project include: synthesis and characterization of the recombinant spider silks and the fibers obtained thereof, evaluation of the mineralization and mineralization control vs. silk composition, structure, reaction conditions. The silk/TiO2/Ag and silk/TiO2/Au hybrid materials are model systems for other complex hybrid materials consisting of multiple components where the functionality depends on the specific arrangements of individual building blocks. Finally, we will investigate the photocatalytic activity of the final hybrid materials as a test reaction for actually generating a functional property from the controlled growth of two inorganic materials on the recombinant templates. The project therefore bridges recombinant protein technology and functional materials research. The question of special interest is whether genetically encoded information in a protein can be used for the generation of complex three-component functional materials and whether it is possible to use genetic engineering to a priori program the final properties and structure of a functional hybrid material into the respective gene(s) even before protein synthesis.

本课题主要以蜘蛛丝为原料合成重组蛋白。具体来说，已知与无机材料（如二氧化钛、银和金）结合的肽序列应纳入蜘蛛丝中。静电纺丝将产生纤维和纤维垫，用作上述无机物矿化的模板/基质。该项目的主要目标包括：重组蛛丝及其纤维的合成和表征，评价其矿化和矿化控制与蛛丝组成、结构、反应条件的关系。丝绸/TiO2/Ag和丝绸/TiO2/Au杂化材料是由多个组分组成的其他复杂杂化材料的模型系统，其功能取决于单个构建块的具体安排。最后，我们将研究最终杂化材料的光催化活性，作为两种无机材料在重组模板上的受控生长实际产生功能特性的测试反应。因此，该项目是重组蛋白技术和功能材料研究的桥梁。特别感兴趣的问题是，蛋白质中的遗传编码信息是否可以用于生成复杂的三组分功能材料，以及是否有可能使用基因工程在蛋白质合成之前将功能杂交材料的最终特性和结构先验地编程到相应的基因中。