Function of Stem cell Zinc Finger 1 (SZF1) / ZNF589 protein in hematopoietic stem cell survival and differentiation

干细胞锌指1（SZF1）/ZNF589蛋白在造血干细胞存活和分化中的作用

• 批准号: 251032075
• 负责人: Dr. Letizia Venturini
• 金额: --
• 依托单位: Klinik für Hämatologie, Hämostaseologie, Onkologie und Stammzelltransplantation
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/251032075?language=en
• 关键词: Function; Stem; cell; Zinc; Finger

The Stem cell Zinc Finger 1 (SZF1)/ZNF589 protein belongs to the large family of KRAB-Zinc Finger transcription factors which epigenetically repress transcription by recruiting chromatin modifying complexes to the promoter region of their target genes. The fact that it was identified as a gene specifically expressed in CD34+ cells, together with the biochemical characteristics of the protein, strongly suggest its implication in epigenetic control of gene expression in hematopoietic stem cells (HSCs). However, the function of SZF1/ZNF589 in hematopoiesis has not yet been studied. My preliminary data support a functional implication of SZF1/ZNF589 in the hematopoietic system. Along with the molecular characterization of SZF1/ZNF589, the aim of this project is to investigate its role in CD34+ cells and to identify its target genes by loss- or gain-of-function studies. The expected results will add new insight into regulatory pathways involved in maintenance, proliferation and differentiation of HSCs, and impact on the understanding of normal and aberrant hematopoiesis.

干细胞锌指1 (SZF1)/ZNF589蛋白属于krab -锌指转录因子大家族，其通过将染色质修饰复合物募集到靶基因的启动子区域来表观遗传地抑制转录。该蛋白被鉴定为CD34+细胞特异性表达的基因，以及该蛋白的生化特性，强烈提示其在造血干细胞（hsc）基因表达的表观遗传控制中具有重要意义。然而，SZF1/ZNF589在造血中的功能尚未被研究。我的初步数据支持SZF1/ZNF589在造血系统中的功能含义。随着SZF1/ZNF589的分子特征，本项目的目的是研究其在CD34+细胞中的作用，并通过功能丧失或功能获得研究确定其靶基因。预期的结果将为造血干细胞的维持、增殖和分化的调控途径提供新的见解，并对正常和异常造血的理解产生影响。