Elucidating function of the novel PINX1-related protein, Chigno, in reproductive stem cells and development
阐明新型 PINX1 相关蛋白 Chigno 在生殖干细胞和发育中的功能
基本信息
- 批准号:10730857
- 负责人:
- 金额:$ 43.35万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-09-01 至 2026-08-31
- 项目状态:未结题
- 来源:
- 关键词:AdultApicalAuthorshipBasic ScienceBehaviorBindingBinding ProteinsCell Differentiation processCell MaintenanceCell physiologyCellsCessation of lifeChronologyCommunicationContraceptive methodsCystDataDaughterDevelopmentDrosophila genusDrosophila melanogasterEducational process of instructingEmbryoEnsureEnvironmentEpithelial CellsEquilibriumFertilityFosteringFutureGene ExpressionGenesGerm CellsGerm cell tumorGoalsGonadal structureGrantHomeostasisHumanHuman DevelopmentInfertilityLinkMaintenanceMalignant NeoplasmsMammalsMapsMethodologyMolecularMorphogenesisOrganOrganismOvaryPatternProcessProductionProteinsRecordsRegulationRepressionResearchResearch PersonnelResearch TrainingResourcesRoleScholarshipStudentsSystemTestingTestisTissuesTrainingTumor Suppressor ProteinsWorkcareercell behaviordevelopmental diseaseembryo tissueexperimental studyflygene regulatory networkgermline stem cellshuman diseasein vivoknock-downneuronal cell bodynovelpreventprogramsprotein functionprotein protein interactionreproductivesenescencestem cell nichestem cell self renewalstem cellsstudent participationstudent trainingsuccesssymposiumtraining opportunitytranscription factortumorundergraduate student
项目摘要
PROJECT SUMMARY:
The germline stem cell (GSC) niche functions to ensure continuous gamete production in organisms that
reproduce sexually. Dysregulation of stem cells has also been linked to developmental disorders, cancer,
infertility, and senescence. In flies and mammals alike, communication between GSCs and the surrounding
soma regulates the balance between stem cell maintenance and differentiation. However, new and
unexplored mechanisms regulating these processes are still being discovered - necessitating further enquiry.
This proposal strives to elucidate the function of a novel PINX1-related protein, CG11180/Childless Gambino
(Chigno), in regulating reproductive stem cell behavior and development in the fruit fly, Drosophila
melanogaster.
Chigno is a novel G-patch domain-containing protein in flies that is related to the mammalian tumor
suppressor, PINX1. We previously identified Chigno as a binding partner of Chronologically inappropriate
morphogenesis (Chinmo), a key regulator of somatic stem cell behavior in the adult Drosophila testis.
Preliminary data collected by undergraduate and master’s student researchers in our lab show that Chigno is
expressed in larval and adult testes, as well as Drosophila ovaries and early embryos. In adult testes, Chigno
is observed specifically at the testis apex, which houses the germline stem cell (GSC) niche. Here, it is
detected both in germ cells as well as somatic cyst stem cells (CySCs) and their progeny which regulate
germline behavior. We also find that Chigno knockdown in adult CySCs and early cyst cells results in CySC
over-production, formation of germ cell tumors, and an expansion of epithelial cells at the testis apex that may
be linked to altered CySC identity. Furthermore, somatic Chigno depletion causes both testis and ovaries to
become infertile.
Experiments in this proposal are focused on understanding the role of Chigno in regulating testis stem
cell self-renewal, differentiation, and cell fate maintenance (Aim 1), understanding Chigno protein function at
the molecular level (Aim 2), and assessing whether Chigno functions in tissues other than the testis, including
Drosophila ovaries and embryonic tissues (Aim 3). Given Chigno’s impact on fertility and testes stem cell
behavior, as well as the diverse functions of G-patch/PINX1-related proteins in a range of cellular processes,
the proposed studies into Chigno function hold great promise for elucidating novel mechanisms controlling
fertility, stem cell behavior, and development in a range of organisms.
项目总结:
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
MATTHEW J WAWERSIK其他文献
MATTHEW J WAWERSIK的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('MATTHEW J WAWERSIK', 18)}}的其他基金
Drosophila melanogaster p53 during gonadogenesis
性腺发生过程中的果蝇 p53
- 批准号:
6767808 - 财政年份:2002
- 资助金额:
$ 43.35万 - 项目类别:
Drosophila melanogaster p53 during gonadogenesis
性腺发生过程中的果蝇 p53
- 批准号:
6640585 - 财政年份:2002
- 资助金额:
$ 43.35万 - 项目类别:
Drosophila melanogaster p53 during gonadogenesis
性腺发生过程中的果蝇 p53
- 批准号:
6552596 - 财政年份:2002
- 资助金额:
$ 43.35万 - 项目类别:
相似国自然基金
FGF8通过Ras/MEK/ERK信号通路调控apical ES结构影响精子生成的机制研究
- 批准号:81801519
- 批准年份:2018
- 资助金额:21.0 万元
- 项目类别:青年科学基金项目
相似海外基金
Changes in apical cochlear mechanics after cochlear implantation
人工耳蜗植入后耳蜗顶端力学的变化
- 批准号:
10730981 - 财政年份:2023
- 资助金额:
$ 43.35万 - 项目类别:
Structural diversity of ceramide moiety responsible for apical membrane function of bladder transitional epithelial cells
负责膀胱移行上皮细胞顶膜功能的神经酰胺部分的结构多样性
- 批准号:
23K08792 - 财政年份:2023
- 资助金额:
$ 43.35万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Establishment of photodynamic diagnosis for apical periodontitis based on 5-ALA fluorescence live imaging
基于5-ALA荧光实时成像的根尖周炎光动力诊断方法的建立
- 批准号:
23K09188 - 财政年份:2023
- 资助金额:
$ 43.35万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Epithelial apical membrane polarization, morphogenesis, and regulation of gene expression
上皮顶膜极化、形态发生和基因表达调控
- 批准号:
BB/X000575/1 - 财政年份:2023
- 资助金额:
$ 43.35万 - 项目类别:
Research Grant
Unveiling Functional Roles of Apical Surface Interactions Between Opposing Cell Layers
揭示相对细胞层之间顶端表面相互作用的功能作用
- 批准号:
10629101 - 财政年份:2023
- 资助金额:
$ 43.35万 - 项目类别:
Cell-type specific assembly of apical extracellular matrices
顶端细胞外基质的细胞类型特异性组装
- 批准号:
10749768 - 财政年份:2023
- 资助金额:
$ 43.35万 - 项目类别:
Evaluation of Trigeminal Ganglia Sensory Neuronal Population/s Mediating MIF-Induced Anti-Nociception in a Model of Apical Periodontitis.
根尖周炎模型中三叉神经节感觉神经元群介导 MIF 诱导的抗伤害感受的评估。
- 批准号:
10822712 - 财政年份:2023
- 资助金额:
$ 43.35万 - 项目类别:
Exploring the role of phosphoinositides in the trafficking of proteins to the apical complex in the malaria parasite Plasmodium falciparum.
探索磷酸肌醇在疟原虫恶性疟原虫顶复合体蛋白质运输中的作用。
- 批准号:
495093 - 财政年份:2023
- 资助金额:
$ 43.35万 - 项目类别:
Operating Grants
Étude du rôle de la phosphatase de phosphoinositides SAC1 dans le trafic de protéines au complexe apical chez le parasite de la malaria Plasmodium falciparum
疟疾疟原虫顶端寄生虫复合物中磷酸肌醇磷酸酶 SAC1 的研究
- 批准号:
486094 - 财政年份:2022
- 资助金额:
$ 43.35万 - 项目类别:
Studentship Programs
Illuminating apical extracellular matrix structure and biogenesis
阐明顶端细胞外基质结构和生物发生
- 批准号:
10654029 - 财政年份:2022
- 资助金额:
$ 43.35万 - 项目类别: