Magnetic Ionic Liquids as Tunable Solvents for Chemical Analysis

本项目由化学学部化学测量与成像（CMI）项目资助。爱荷华州立大学的Jared L. Anderson教授正在开发化学测量的新方法和平台，利用磁性离子液体（mil）在化学分离中的物理和化学特性。mil是一种非分子溶剂，具有低熔点，可调粘度，并且可以很容易地使用外加磁场进行操作。这项工作有可能为从临床样本中分析核酸提供额外的方法，并可能改变许多跨学科领域，包括临床诊断、生物医学和法医学研究。研究生和本科生在爱荷华州立大学文科与理科院长创新与创业学院开发的模块中学习分离科学和生物分析化学，鼓励创新和创业。高中生通过美国化学会项目SEED计划参与研究。一个社会网络程序被用来向儿童和普通大众传播科学视频。这一建议集中在三个领域，磁性离子液体的研究和用于核酸的化学测量。磁性离子液体具有更强的磁性，被设计并应用于生物分析分析和色谱分离中；开发了使用mil捕获相对于生物样品中其他组分的核酸的方法；研究了使用3D打印的新型化学测量平台，以区分含有单核苷酸多态性的核酸序列。3D打印液滴发生器提供液滴尺寸和体积的定制，并扩展到在含水样品中生产均匀的MIL液滴。这项研究提供了关于这些材料的基本新知识，并催化了实现快速和选择性核酸分析的新方法的发展。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

This project is funded by the Chemical Measurement and Imaging (CMI) program in the Division of Chemistry. Professor Jared L. Anderson of Iowa State University is developing new approaches and platforms for chemical measurement that exploit the physical and chemical properties of magnetic ionic liquids (MILs) in chemical separations. MILs are non-molecular solvents that possess low melting points, tunable viscosities, and can be readily manipulated using an applied magnetic field. The proposed work has the potential to provide additional approaches by which nucleic acids are analyzed from clinical samples, and could transform numerous interdisciplinary fields including clinical diagnostics, biomedicine, and forensics research. Graduate and undergraduate students acquire training in separation science and bioanalytical chemistry in an environment that teaches and encourages innovation and entrepreneurship through modules developed with the Liberal Arts and Sciences Dean’s Innovation and Entrepreneurship Academy at Iowa State University. High school students participate in the research through the American Chemical Society Project SEED program. A social networking program is used to disseminate scientific videos to young children and the general public. This proposal focuses on three areas where magnetic ionic liquids are studied and used in the chemical measurement of nucleic acids. Magnetic ionic liquids with stronger magnetic properties are designed and incorporated into bioanalytical assays and chromatographic separations; approaches using MILs for the capture of nucleic acids relative to other components in biological samples are developed; and new chemical measurement platforms using 3D printing are studied to enable differentiation of nucleic acid sequences that contain single-nucleotide polymorphisms. 3D printed droplet generators provide customization of droplet size and volume and are extended to the production of uniform MIL droplets within aqueous samples. This research provides fundamental new knowledge about these materials and catalyzes the development of new approaches for achieving rapid and selective nucleic acid analysis.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.