Iron-sulfur Clusters as Biological Sensors: Mechanistic Investigations by Synthetic Modeling Studies
铁硫簇作为生物传感器:通过综合建模研究进行机理研究
基本信息
- 批准号:2203879
- 负责人:
- 金额:$ 45.02万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-07-01 至 2025-06-30
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
With the support of the Chemistry of Life Processes (CLP) program in the Division of Chemistry, Professor Eunsuk Kim from Brown University is studying the development of synthetic modeling systems to understand how iron-sulfur (Fe-S) clusters act as sensors for environmental signals. Iron-sulfur clusters are ubiquitous cofactors present in all animal kingdoms. Organisms have evolved to exploit the inherent reactivity of Fe-S clusters with small molecules like O2 and NO to assess their environment. For example, bacteria sense environmental O2 and NO and adjust their gene expression profiles for survival using regulatory proteins that contain Fe-S clusters. The human Fe-S mitoNEET protein acts as a redox sensor and regulates mitochondrial functions, failure of which leads to metabolic and neurodegenerative diseases. The proposed study will offer molecular insights into the modification and repair of iron-sulfur clusters by small redox signaling molecules. Molecular insights gained in this project will inform research focused on the development of biomarkers for disease detection, of antimicrobial agents, and of therapeutics for metabolic diseases. Students who will implement the research will gain knowledge, expertise and skills in chemical synthesis, reactivity, structural characterization of molecules, and spectroscopy. This project is also integrated into an outreach program focused on the improvement of the educational environments of students from groups underrepresented in STEM (science, technology, engineering and mathematics) and students from public high schools. This research project seeks to understand strategies used by Fe-S regulatory proteins to battle oxidative and nitrosative stress at the molecular level. This goal is achieved by studying the geometric and electronic structure, reactivity, and bonding properties of discrete biomimetic, model Fe-S complexes. The chemical reactivity of Fe-S clusters will be studied in three different areas. Firstly, mechanistic details for the reactions of 4Fe-4S clusters with NO will be examined. These include the characterization of novel iron nitrosyl products and investigations on how reactive sulfur species are generated through the action of NO upon Fe-S clusters. Secondly, mitoNEET model clusters will be synthesized and bonding properties of the Fe-S cluster will be correlated to cluster transfer activity. Lastly, persulfide-ligated Fe-S clusters will be synthesized used to study the role of persulfides in the Fe-S repair process. Overall then, these fundamental mechanistic and spectroscopic studies have real promise to enhance understanding of Fe-S complexes in biology, in general, and their specific roles in electron transfer-initiated processes including interactions with signaling molecules such as NO and persulfides.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
在化学系生命过程化学(CLP)项目的支持下,布朗大学的Eunsuk Kim教授正在研究合成建模系统的开发,以了解铁硫(Fe-S)簇如何作为环境信号的传感器。铁硫簇是所有动物界普遍存在的辅因子。生物体已经进化到利用Fe-S簇与O2和NO等小分子的固有反应性来评估其环境。例如,细菌感知环境中的O2和NO,并使用含有Fe-S簇的调节蛋白来调节它们的基因表达谱以存活。人Fe-S mitoNEET蛋白充当氧化还原传感器并调节线粒体功能,线粒体功能的失效导致代谢和神经退行性疾病。这项研究将为小氧化还原信号分子对铁硫簇的修饰和修复提供分子见解。在该项目中获得的分子见解将为专注于开发用于疾病检测的生物标志物、抗菌剂和代谢疾病治疗剂的研究提供信息。谁将实施研究的学生将获得化学合成,反应性,分子结构表征和光谱学方面的知识,专业知识和技能。该项目还纳入了一个外联方案,重点是改善STEM(科学、技术、工程和数学)代表性不足群体的学生和公立高中学生的教育环境。该研究项目旨在了解Fe-S调节蛋白在分子水平上对抗氧化和亚硝化应激的策略。 这一目标是通过研究离散仿生模型Fe-S复合物的几何和电子结构、反应性和成键性质来实现的。Fe-S团簇的化学反应性将在三个不同的领域进行研究。首先,4Fe-4S团簇与NO反应的机理细节将被检查。这些包括新的铁亚硝酰基产品的表征和调查如何反应性硫物种是通过对Fe-S簇的NO的作用产生的。其次,我们将合成mitoNEET模型团簇,并将Fe-S团簇的成键性质与团簇转移活性关联起来。最后,合成了过硫化物配位的Fe-S簇合物,用于研究过硫化物在Fe-S修复过程中的作用。 总的来说,这些基本的机理和光谱研究有真实的希望加强对生物学中Fe-S复合物的理解,以及它们在电子转移中的特殊作用该奖项反映了NSF的法定使命,并通过使用基金会的知识价值和更广泛的影响审查进行评估,被认为值得支持的搜索.
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Eunsuk Kim其他文献
Soil and Vegetation Characteristics Associated with the Invasion of Humulus japonicus in Riparian Zones
- DOI:
10.1007/s12374-025-09478-4 - 发表时间:
2025-07-18 - 期刊:
- 影响因子:2.000
- 作者:
Haeji Shin;Tae-Min Kim;Eunsuk Kim - 通讯作者:
Eunsuk Kim
Assessment of colloidal copper speciation in the Mekong River Delta using diffusive gradients in thin film techniques
使用薄膜技术中的扩散梯度评估湄公河三角洲的胶体铜形态
- DOI:
10.1016/j.ecss.2017.01.014 - 发表时间:
2017 - 期刊:
- 影响因子:2.8
- 作者:
Kahyee Cary Seah;G. Qasim;Yongseok Hong;Eunsuk Kim;Kyunghun Kim;Seunghee Han - 通讯作者:
Seunghee Han
Acid-dependent degradation of a [2Fe-2S] cluster by nitric oxide.
一氧化氮对 [2Fe-2S] 簇的酸依赖性降解。
- DOI:
- 发表时间:
2012 - 期刊:
- 影响因子:4.6
- 作者:
Camly T Tran;Eunsuk Kim - 通讯作者:
Eunsuk Kim
Coordination-triggered NO release from a dinitrosyl iron complex leads to anti-inflammatory activity
二亚硝基铁络合物协调触发 NO 释放,具有抗炎活性
- DOI:
- 发表时间:
2014 - 期刊:
- 影响因子:0
- 作者:
Kelsey M. Skodje;Min‐Young Kwon;S. Chung;Eunsuk Kim - 通讯作者:
Eunsuk Kim
Eunsuk Kim的其他文献
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{{ truncateString('Eunsuk Kim', 18)}}的其他基金
Chemistry of Iron-sulfur clusters in redox signaling
氧化还原信号传导中铁硫簇的化学
- 批准号:
1807845 - 财政年份:2018
- 资助金额:
$ 45.02万 - 项目类别:
Standard Grant
CAREER: Chemistry of Iron-Sulfur Clusters in Nitric Oxide Signaling
职业:一氧化氮信号传导中铁硫簇的化学
- 批准号:
1254733 - 财政年份:2013
- 资助金额:
$ 45.02万 - 项目类别:
Continuing Grant
相似国自然基金
内源性二氧化硫对低氧性肺血管基质重塑的调节作用及机制
- 批准号:81070111
- 批准年份:2010
- 资助金额:35.0 万元
- 项目类别:面上项目
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