Escalation in coevolution: Characterization of novel plant toxins, how specialist herbivores cope, and tests of phylogenetic intensification in plant defense potency

共同进化的升级:新型植物毒素的表征、专业食草动物如何应对以及植物防御效力的系统发育强化测试

基本信息

  • 批准号:
    2209762
  • 负责人:
  • 金额:
    $ 65.77万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2022
  • 资助国家:
    美国
  • 起止时间:
    2022-06-01 至 2025-05-31
  • 项目状态:
    未结题

项目摘要

Insects that feed on plants are thought to impose natural selection for chemical defenses in the plant tissues they eat. The research being conducted here is on milkweed plants, which contains a highly potent toxin, labriformin, which has evolved to high concentrations in in several species. When highly expressed, labriformin inhibits an essential transport enzyme (the sodium-potassium pump) of the monarch butterfly and the large milkweed bug. These specialized insect pests biochemically modify labriformin to less toxic compounds and sequester these-products for their own defense. The monarch and seed bug’s highly tolerant sodium pump and biochemical modifications point to labriformin being at the center of reciprocal evolution between milkweed and its herbivores. This work serves to unravel the mechanisms of the interaction through physiological and evolutionary analyses. The research also serves as a model for understanding both natural and agricultural interactions between plants and pests. Finally, the milkweed - monarch system lends itself well to public engagement because of its iconic stature, declining populations of conservation concern, and use in school curricula. As part of this project, the researchers are developing modules for K-12 schools and engaging in public outreach through presentations and blogs. Among milkweed plants which are fed upon by several specialized lineages of insect herbivores, coevolutionary interactions have been well-studied and novel mechanisms have been elucidated. Researchers recently discovered a milkweed that contains high levels of labriformin, a highly toxic cardenolide which is unusual in form (containing a ring with nitrogen (N) and sulfur (S)), which could well be an escalated form of costly defense. The current work seeks to investigate additional N-containing cardenolides across the milkweed phylogeny and seek to decipher their physiological impacts and detoxification in independent herbivores lineages. Repeatedly evolving offense-defense mechanisms in plant-herbivore lineages will likely reveal generalities of coevolution. Three objectives will address both sides of coevolution: 1) Determine the mechanisms by which N-containing cardenolide defenses are processed, detoxified, and stored by two specialist milkweed insects, monarch caterpillars and seed bugs. This will employ feeding trials with isolated compounds, physiological assays, and studies of behavior, using both specialists as well as CRISPR-edited Drosophila that have genetic substitutions for tolerating cardenolides. 2) Assess the costs and benefits of detoxification and sequestration using quantitative genetics coupled with feeding and chemical assays of monarchs and seed bugs on milkweed plants with and without N-containing cardenolides. Genetic correlations will reveal life-history costs and traits associated with differential sequestration. 3) Examine the pattern of N-containing defense evolution among milkweeds across the phylogeny, testing hypotheses about N-limitation, defense allocation, and trade-offs between cardenolide concentration and potency. One hypothesis, that later diverging milkweeds have evolved more potent but less concentrated cardenolides, was predicted by theory and suggested by a phylogenetic pattern, but never rigorously tested. Overall these objectives will advance understanding of coevolutionary mechanisms by characterizing novel plant defenses and the physiological ecology of counter-adaptation in specialist herbivores, genetic variation in detoxification and sequestration of toxins, and plant defense evolution at the macroevolutionary scale.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
以植物为食的昆虫被认为是对它们所吃的植物组织中的化学防御进行自然选择。这里正在进行的研究是在乳草植物上进行的,乳草植物含有一种非常有效的毒素,labrivaline,它在几个物种中已经进化到高浓度。当高表达时,labrivaline抑制帝王蝶和大型马利筋虫的必需运输酶(钠-钾泵)。这些专门的昆虫害虫生物化学修改labrivaline毒性较低的化合物和螯合这些产品为自己的防御。帝王蝶和种子虫对钠泵的高度耐受性和生化修饰表明,金龟子是马利筋和其食草动物之间相互进化的中心。这项工作旨在通过生理和进化分析来揭示这种相互作用的机制。这项研究也是了解植物和害虫之间自然和农业相互作用的模型。最后,马利筋-帝王蝶系统很适合公众参与,因为它的标志性地位,保护关注的人口下降,并在学校课程中使用。作为该项目的一部分,研究人员正在为K-12学校开发模块,并通过演讲和博客参与公共宣传。马利筋属植物是几种专门的食草性昆虫类群的食物来源,它们之间的共同进化相互作用已被深入研究,并阐明了新的机制。研究人员最近发现了一种乳草,其中含有高水平的labrivaline,这是一种剧毒的cardenaline,其形式不寻常(含有氮(N)和硫(S)的环),这很可能是一种升级形式的昂贵防御。目前的工作旨在调查更多的含N cardenolides在马利筋的发育,并试图破译其生理影响和解毒独立的食草动物谱系。在植物-草食动物谱系中反复进化的进攻-防御机制可能会揭示共同进化的普遍性。三个目标将解决共同进化的两个方面:1)确定含N的cardenoprotein防御被两种专业马利筋昆虫,君主毛虫和种子错误处理,解毒和储存的机制。这将采用分离化合物的喂养试验,生理测定和行为研究,使用专家以及CRISPR编辑的果蝇,这些果蝇具有耐受cardenolides的基因替代。2)评估解毒和螯合的成本和效益,使用定量遗传学加上饲养和化学测定的君主和种子错误的马利筋植物含氮和不含强心内酯。遗传相关性将揭示生活史成本和与差异螯合相关的特征。3)检查模式的含N的防御进化马利筋在整个生育期,测试有关N-限制,防御分配的假设,和cardenopropylene浓度和效力之间的权衡。有一种假说认为,后来分化的乳草已经进化出了更有效但浓度更低的强心内酯,这是理论预测和系统发育模式的建议,但从未经过严格的检验。总的来说,这些目标将通过表征新的植物防御和专业食草动物的反适应生理生态学,解毒和螯合毒素的遗传变异,该奖项反映了NSF的法定使命,并通过使用基金会的知识价值和更广泛的影响进行评估,被认为值得支持审查标准。

项目成果

期刊论文数量(16)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Plant defense synergies and antagonisms affect performance of specialist herbivores of common milkweed
  • DOI:
    10.1002/ecy.3915
  • 发表时间:
    2022-12-26
  • 期刊:
  • 影响因子:
    4.8
  • 作者:
    Edwards, Collin B.;Ellner, Stephen P.;Agrawal, Anurag A.
  • 通讯作者:
    Agrawal, Anurag A.
A nutrition–defence trade-off drives diet choice in a toxic plant generalist
营养与防御的权衡推动了有毒植物多面手的饮食选择
The Distribution of Species Interactions
  • DOI:
    10.1086/727939
  • 发表时间:
    2023-12
  • 期刊:
  • 影响因子:
    0
  • 作者:
    R. Karban;Anurag A. Agrawal
  • 通讯作者:
    R. Karban;Anurag A. Agrawal
A direct comparison of ecological theories for predicting the relationship between plant traits and growth
  • DOI:
    10.1002/ecy.3986
  • 发表时间:
    2023-03-06
  • 期刊:
  • 影响因子:
    4.8
  • 作者:
    Goud, Ellie M.;Agrawal, Anurag A.;Sparks, Jed P.
  • 通讯作者:
    Sparks, Jed P.
Plant water‐use strategies as mediators of herbivore drought response: Ecophysiology, host plant quality and functional traits
  • DOI:
    10.1111/1365-2745.14059
  • 发表时间:
    2022-12
  • 期刊:
  • 影响因子:
    5.5
  • 作者:
    A. N. Carvajal Acosta;A. Agrawal;Kailen A. Mooney
  • 通讯作者:
    A. N. Carvajal Acosta;A. Agrawal;Kailen A. Mooney
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Anurag Agrawal其他文献

Exception Handling in the Spreadsheet Paradigm
电子表格范例中的异常处理
  • DOI:
    10.1109/32.879817
  • 发表时间:
    2000
  • 期刊:
  • 影响因子:
    0
  • 作者:
    M. Burnett;Anurag Agrawal;P. V. Zee
  • 通讯作者:
    P. V. Zee
Publisher Correction: Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software
出版商更正:超分辨率搏击俱乐部:2D 和 3D 单分子定位显微镜软件的评估
  • DOI:
    10.1038/s41592-019-0453-4
  • 发表时间:
    2019
  • 期刊:
  • 影响因子:
    48
  • 作者:
    D. Sage;Thanh;H. Babcock;T. Lukes;T. Pengo;J. Chao;Ramraj Velmurugan;Alex D Herbert;Anurag Agrawal;S. Colabrese;A. Wheeler;Anna Archetti;B. Rieger;R. Ober;G. Hagen;J. Sibarita;J. Ries;Ricardo Henriques;M. Unser;S. Holden
  • 通讯作者:
    S. Holden
Topical application of aerial portion of emAcalypha indica/em Linn ameliorates psoriasis in rodents: Evidences from emin vivo/em and emin silico/em studies
印度铁苋菜地上部分局部应用可改善啮齿动物银屑病:体内和计算机模拟研究的证据
  • DOI:
    10.1016/j.jep.2023.116685
  • 发表时间:
    2023-10-28
  • 期刊:
  • 影响因子:
    5.400
  • 作者:
    Anurag Agrawal;Giriraj T. Kulkarni;Lakshmayya
  • 通讯作者:
    Lakshmayya
Mitochondrial donation by stem cells: potential for novel therapeutics
  • DOI:
    10.1186/1755-8166-7-s1-i59
  • 发表时间:
    2014-01-21
  • 期刊:
  • 影响因子:
    1.400
  • 作者:
    Anurag Agrawal
  • 通讯作者:
    Anurag Agrawal
Likelihood of False-Positive Results in High-Impact Journals Publishing Groundbreaking Research
发表突破性研究的高影响力期刊出现假阳性结果的可能性
  • DOI:
  • 发表时间:
    2012
  • 期刊:
  • 影响因子:
    3.1
  • 作者:
    Anurag Agrawal;A. Sharma
  • 通讯作者:
    A. Sharma

Anurag Agrawal的其他文献

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{{ truncateString('Anurag Agrawal', 18)}}的其他基金

Collaborative Research: Insect herbivore feeding guilds and compartmentalized plant defense
合作研究:昆虫食草动物喂养协会和分区植物防御
  • 批准号:
    1907491
  • 财政年份:
    2019
  • 资助金额:
    $ 65.77万
  • 项目类别:
    Standard Grant
SBIR Phase II: Three-Dimensional Computational Optical Imaging Sensor
SBIR 第二阶段:三维计算光学成像传感器
  • 批准号:
    1534745
  • 财政年份:
    2015
  • 资助金额:
    $ 65.77万
  • 项目类别:
    Standard Grant
EAGER: Genetic transformation of common milkweed, Asclepias syriaca: Creating a model plant for ecological investigations
EAGER:常见马利筋(Asclepias syriaca)的遗传转化:创建用于生态研究的模型植物
  • 批准号:
    1513839
  • 财政年份:
    2015
  • 资助金额:
    $ 65.77万
  • 项目类别:
    Standard Grant
SBIR Phase II: Widefield Three-Dimensional Superresolution Microscopy Module
SBIR 第二阶段:宽视场三维超分辨率显微镜模块
  • 批准号:
    1353638
  • 财政年份:
    2014
  • 资助金额:
    $ 65.77万
  • 项目类别:
    Standard Grant
SBIR Phase I: Three-Dimensional Computational Optical Imaging Sensor
SBIR 第一阶段:三维计算光学成像传感器
  • 批准号:
    1346142
  • 财政年份:
    2014
  • 资助金额:
    $ 65.77万
  • 项目类别:
    Standard Grant
SBIR Phase I: Widefield 3D Superresolution Microscopy Module
SBIR 第一阶段:宽场 3D 超分辨率显微镜模块
  • 批准号:
    1248873
  • 财政年份:
    2013
  • 资助金额:
    $ 65.77万
  • 项目类别:
    Standard Grant
DISSERTATION RESEARCH: Consequences of Indirect Defensive Traits on Microbial Diversity
论文研究:间接防御特征对微生物多样性的影响
  • 批准号:
    1209031
  • 财政年份:
    2012
  • 资助金额:
    $ 65.77万
  • 项目类别:
    Standard Grant
Tests of Classic Theory in Plant-insect Interactions
植物-昆虫相互作用经典理论的检验
  • 批准号:
    1118783
  • 财政年份:
    2011
  • 资助金额:
    $ 65.77万
  • 项目类别:
    Standard Grant
COLLABORATIVE RESEARCH: EAGER: Evolution of Plant Defense: A Multigenerational Selection Experiment in the Field
合作研究:EAGER:植物防御的进化:田间多代选择实验
  • 批准号:
    0950231
  • 财政年份:
    2009
  • 资助金额:
    $ 65.77万
  • 项目类别:
    Standard Grant
Workshop: Frontiers in Ecology: January 12-13, 2006, at NSF
研讨会:生态学前沿:2006 年 1 月 12 日至 13 日,美国国家科学基金会 (NSF)
  • 批准号:
    0544929
  • 财政年份:
    2005
  • 资助金额:
    $ 65.77万
  • 项目类别:
    Standard Grant

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多盘科单殖吸虫宿主特异性及其与无尾两栖类宿主协同进化关系研究
  • 批准号:
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NSF Postdoctoral Fellowship in Biology: Human Domestication of Maize as Bio-cultural Coevolution
美国国家科学基金会生物学博士后奖学金:人类驯化玉米作为生物文化协同进化
  • 批准号:
    2305694
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    2024
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CAREER: Understanding biosphere-geosphere coevolution through carbonate-associated phosphate, community archives, and open-access education in rural schools
职业:通过碳酸盐相关磷酸盐、社区档案和农村学校的开放教育了解生物圈-地圈协同进化
  • 批准号:
    2338055
  • 财政年份:
    2024
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    $ 65.77万
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    Continuing Grant
Postdoctoral Research Fellowship in Biology: The Coevolution of Climate Change Responses in Grasses
生物学博士后研究金:草类气候变化响应的共同进化
  • 批准号:
    2209408
  • 财政年份:
    2023
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    $ 65.77万
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Investigation of responsible genetic basis for male and female coevolution of exaggerated genitalia
夸张生殖器男女共同进化的遗传基础研究
  • 批准号:
    22KJ3101
  • 财政年份:
    2023
  • 资助金额:
    $ 65.77万
  • 项目类别:
    Grant-in-Aid for JSPS Fellows
Genetic basis of rapid coevolution of morphological traits between sexes
两性形态性状快速协同进化的遗传基础
  • 批准号:
    23H02530
  • 财政年份:
    2023
  • 资助金额:
    $ 65.77万
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    Grant-in-Aid for Scientific Research (B)
Development of high-resolution X-ray telescope for studying the coevolution of galaxies and black holes
开发用于研究星系和黑洞共同演化的高分辨率X射线望远镜
  • 批准号:
    23H00128
  • 财政年份:
    2023
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    $ 65.77万
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Inferring the gene coevolution network from deep comparative genomics
从深度比较基因组学推断基因协同进化网络
  • 批准号:
    2241312
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    2023
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    $ 65.77万
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Discover and verify principles of coevolution using laboratory evolution accelerated by transposons
利用转座子加速的实验室进化发现并验证共同进化的原理
  • 批准号:
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Revealing coevolution of supermassive black holes and host galaxies with the gravitational wave background
引力波背景揭示超大质量黑洞与宿主星系的协同演化
  • 批准号:
    23K03460
  • 财政年份:
    2023
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    $ 65.77万
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    Grant-in-Aid for Scientific Research (C)
Causes and consequences of intra-genomic coevolution
基因组内协同进化的原因和后果
  • 批准号:
    10644124
  • 财政年份:
    2023
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    $ 65.77万
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