Rational Design of Trifunctional Polyen Dyes for Highly Sensitive, Multiplexed Bioanalytics with Surface-Enhanced Resonance Raman Scattering

用于表面增强共振拉曼散射高灵敏度多重生物分析的三官能多烯染料的合理设计

• 批准号: 251872094
• 负责人: Professor Dr. Sebastian Schlücker
• 金额: --
• 依托单位: Physikalische Chemie - AG Schlücker
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/251872094?language=en
• 关键词: Rational; Design; Trifunctional; Polyen; Dyes

Functionalized noble metal colloids have an enormous potential for quantitative, highly sensitive, multiplexed bioanalytics with surface-enhanced (resonance) Raman scattering (SE(R)RS). Multiplexing capacity, quantification, sensitivity, and stability of such SE(R)RS particles largely depend on the properties of the Raman reporter molecules on the surface of the metal colloid. However, concepts for tailor-made Raman reporters, which exhibit all desired physical and chemical properties in a single molecular lead structure, are currently not available. The central goal of this project is the rational design and synthesis of trifunctional Raman dyes with high local symmetry, control over the position of marker peaks, maximum Raman scattering cross sections, high surface affinity with minimal distance, maximum surface coverage and units for hydrophilic stabilization and bioconjugation. The selection and synthesis of at least 10 different Raman reporter dyes is guided by theory based on DFT calculations of the corresponding vibrational spectrum. Signal strength, reproducibility, and quantification of the new SERRS particles are tested first in defined colloidal mixtures and then in a 10-plex-immuno-SERRS assay.

功能化贵金属胶体具有巨大的潜力，可用于表面增强(共振)拉曼散射(SE(R)RS)的定量、高灵敏度、多路生物分析。这种SE(R)RS粒子的多路复用能力、量化、灵敏度和稳定性在很大程度上取决于金属胶体表面的拉曼报告分子的性质。然而，在单个分子铅结构中展示所有所需物理和化学性质的定制拉曼报告器的概念目前还不存在。该项目的中心目标是合理地设计和合成具有高局部对称性的三官能团拉曼染料，控制标记峰的位置，最大的拉曼散射截面，最小距离的高表面亲和力，最大的表面覆盖率和亲水稳定和生物结合单元。至少10种不同的拉曼报告染料的选择和合成是以相应振动光谱的密度泛函理论计算为指导的。首先在确定的胶体混合物中测试新的SERRS颗粒的信号强度、重复性和量化，然后在10-plex免疫SERRS分析中进行测试。

项目成果

Rational design of thiolated polyenes as trifunctional Raman reporter molecules in surface‐enhanced Raman scattering nanotags for cytokine detection in a lateral flow assay

• DOI: 10.1002/jbio.201960126
• 发表时间: 2020-01
• 期刊: Journal of Biophotonics
• 影响因子: 2.8
• 作者: Thomas Keller;Svetlana Brem;Vi Tran;Oliver Sritharan;D. Schäfer;S. Schlücker