EFRI ELiS: Bacteria-Bacteriophage Hybrid System for Rare Earth Element Biomining

稀土元素（REEs）被用作广泛的基本产品和清洁能源技术的关键成分，包括智能手机、医疗成像设备（MRI）、太阳能电池、电动汽车、风力涡轮机和发光二极管（LED）。在美国，超过90%的商业产品和工业应用中使用的稀土元素是通过开采、提取和加工原生矿石在海外生产的。然而，稀土开采具有严重的环境足迹，因为它需要大量的能源和水，并产生大量的废物。该项目的总体目标是探索开发一种综合生物采矿工艺，用于从国内矿石、矿床和废料中可持续地提取、分离和浓缩稀土元素。为了推进这一目标，主要研究人员建议设计和构建一种混合细菌和工程噬菌体系统，该系统可以1）从加工的矿石，矿物，固体废物中提取稀土元素，2）从所得浸出液中分离稀土元素，以生产用于工业用途的纯化稀土浓缩物。这项研究的成功完成将通过产生新的基础知识来促进下一代生物采矿工艺的发展，以可持续地提取和纯化稀土元素，从而造福社会。通过教育和培训，包括指导加州大学的两名研究生和一名博士后研究员，将为社会带来更多的好处，美国很容易受到稀土元素供应中断的影响它们已成为广泛的基本工业产品和清洁能源技术的关键组成部分。因此，迫切需要通过可持续地开采和加工国内稀土矿床来减少国家对进口稀土的依赖。本研究的目的是开发一种新型的生物湿法冶金工艺，包括一个集成的细菌和工程噬菌体（噬菌体）的混合系统，可以持续生物浸出稀土从他们的矿石和分离/浓缩的稀土浸出液。为了以具有成本效益和可持续的方式回收稀土，合成生物学方法将与工程甲基营养菌相结合，以选择性地生物积累稀土磷酸盐和工程E。大肠杆菌生产有机酸用于生物浸出。 本研究的具体目标是：（1）寻找和设计与稀土结合和转运有关的蛋白质，（2）构建与稀土结合的噬菌体，用于稀土的选择性分离，（3）将工程菌与噬菌体结合，构建稀土复合提取体系，（4）构建稀土复合提取体系，（5）构建稀土复合提取体系，（6）构建稀土复合提取体系，（7）构建稀土复合提取体系，（8）构建稀土复合提取体系，（9）构建稀土复合提取体系，（10）构建稀土复合提取体系。（4）研究稀土生物采矿新工艺的社会、经济、政治和环境影响。这项研究的成功完成有可能通过开发具有成本效益和环境可接受的工艺，从加工的矿石和废料中提取，分离和浓缩稀土，对生物采矿产生变革性影响。为了实现本项目的教育和培训目标，主要研究者建议利用各自机构的现有计划和资源开发和实施两个计划，包括本科生生物学/生物工程/生物采矿研究（REU）计划和帮助（BIO-RUSH），一个教育/推广STEM计划，旨在招募和指导K-12学生和本科生/该奖项反映了NSF的法定使命，并被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。

Rare earth elements (REEs) are utilized as critical components in a broad range of essential products and clean energy technologies including smart phones, medical imaging equipment (MRI), solar cells, electrical vehicles, wind-turbines, and light emitting diodes (LEDs). In the United States, over 90% of the REEs used in commercial products and industrial applications are produced overseas through the mining, extraction, and processing of virgin ores. However, REE mining has a heavy environmental footprint as it requires significant amounts of energy and water and generates substantial amounts of wastes. The overarching goal of this project is to explore the development of an integrated biomining process for the sustainable extraction, separation, and concentration of REEs from domestic ores, mineral deposits, and waste materials. To advance this goal, the Principal Investigators propose to design and build a hybrid bacterial and engineered bacteriophage system that could 1) extract REEs from processed ores, minerals, solid wastes, and 2) separate the REEs from the resulting leaching solutions to produce purified REE concentrates for industrial usage. The successful completion of this research will benefit society through the generation of new fundamental knowledge to advance the development of next generation biomining processes for the sustainable extraction and purification of REEs. Additional benefits to society will be achieved through education and training including the mentoring of two graduate students and a postdoctoral researcher at the University of California, Berkeley and two graduate students at the University of Arizona.The United States is vulnerable to disruptions in its supply of rare earth elements (REEs) which have become the critical components of a broad range of essential industrial products and clean energy technologies. Thus, there is an urgent need to reduce the Nation’s reliance on imported REEs by sustainably mining and processing domestic deposits of REEs. The goal of this research is to develop a novel bio-hydrometallurgical process consisting of an integrated bacterial and engineered bacteriophage (Phage) hybrid system that could sustainably bioleach REEs from their ores and separate/concentrate the REEs from the leaching solutions. To recover REEs in a cost-effective and sustainable manner, a synthetic biology approach will be integrated with engineered methylotrophic bacteria to selectively bioaccumulate REEs as phosphates and to engineer E. coli to produce organic acids for bioleaching. The specific objectives of the research are to: (1) Discover and design the proteins that are responsible for the binding and transportation of REEs; (2) Engineer phage for REE binding for the selective separation of REEs; (3) Integrate the engineered bacterial strains with phage to build a hybrid REE extraction system; and (4) Investigate the social, economic, political, and environmental implications of the proposed new REE biomining process. The successful completion of this research has the potential for transformative impact on biomining through the development of cost-effective and environmentally acceptable processes to extract, separate, and concentrate REEs from processed ores and waste materials. To implement the educational and training goals of this project, the Principal Investigators propose to leverage existing programs and resources at their respective institutions to develop and implement two programs including a Biology/Bioengineering/Biomining Research for Undergraduate Student (REU) program and Help (BIO-RUSH), an education/outreach STEM program designed to recruit and mentor K-12 students and undergraduate/graduate students from underrepresented groups.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.