EFRI ELiS: Biosynthetic Additive Manufacturing of Living Building Materials

EFRI ELiS：活性建筑材料的生物合成增材制造

• 批准号: 2318057
• 负责人: Jinxing Li
• 金额: $ 200万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2027-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2318057&HistoricalAwards=false
• 关键词: EFRI; ELiS; Biosynthetic; Additive; Manufacturing

This award aims to integrate cutting-edge approaches in advanced manufacturing, synthetic biology, and materials science to transform microbe-engineered lignocellulosic biomass into a printable ink for biosynthetic additive manufacturing of 3D-printed living materials and structures for building applications. The integration of carefully designed microbial networks in printable lignocellulose inks will produce hierarchically structured organic-inorganic living composites with enhanced mechanical and thermal properties, increased carbon storage, self-healing capacity, and scalable and modular fabrication. The award’s vision is that the technical innovations will lead to positive social impacts through: fighting climate change with carbon-negative living materials; combating homelessness through smart and modular building materials production; and filling technical gaps in the nation's Biomanufacturing and Bioeconomy initiative. The research thrusts will be tightly coupled with comprehensive educational and outreach components, including new projects and contents in graduate courses, REU/K-12 activities such as Science Festival showcase, iGEM undergraduate team mentorships, living materials art exhibitions, and biomanufacturing outreach workshops to prepare future scientists and engineers from diverse backgrounds in the highly interdisciplinary research fields of biomanufacturing and living materials.The goal of the research is to adopt ecological concepts of biomineralization and repurpose the symbiotic principles of fungal-bacterial interactions to model and design living microbial networks within lignocellulosic-biomass-derived materials as ink for biosynthetic additive manufacturing and in situ modulation of the material's properties. The research objectives of this project include: 1) ink Development: Design, model, and produce biomass-derived, microbe-integrated, and printable biomaterials feedstock for in situ biomineral and biopolymer synthesis; 2) additive Biomanufacturing: 3D printing with living components to optimize the material's performance and customize the construction of living building materials and structures; 3) benchmarking and Optimization: characterize and optimize the performance of the 3D bioprinted materials and structures, including strength, thermal properties, self-healing capacities, fire resistance, and carbon storage. We will finally scale up ink production with local feedstock, and scale up the printability and biomanufacturing for Environmental Impact Analysis and Techno-Economic Assessment. The overarching focus will be on obtaining a better understanding of how the synergized interactions between microbe-consortia and inert components within the 3D-printed physical-biological system can be engineered to enhance the material's performance. The project will allow the exploitation of microbial interdependencies and fungal-bacteria interactions in novel ways to analyze their habitation, reproduction, metabolism, interaction, and biosafety in an engineered space across different time scales and physical dimensions. The project will also allow the PI to develop a new biomanufacturing process that seamlessly integrates 3D printing and the biosynthetic process for tailored materials design based on highly scalable and sustainable biomass materials as feedstock. Therefore, this project will enable novel strategies to magnify biomanufacturing capacity in hybrid living-manmade systems.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项旨在整合先进制造、合成生物学和材料科学的尖端方法，将微生物工程木质纤维生物质转化为可打印油墨，用于建筑应用的3D打印生物材料和结构的生物合成添加剂制造。将精心设计的微生物网络集成到可打印的木质纤维油墨中，将产生具有增强的机械和热性能、增加碳储存、自我修复能力以及可伸缩和模块化制造的层次化结构有机-无机生物复合材料。该奖项的愿景是，技术创新将通过以下方式产生积极的社会影响：用碳负向生活材料应对气候变化；通过智能和模块化建筑材料生产来解决无家可归问题；以及填补国家生物制造和生物经济倡议中的技术空白。研究的重点将与全面的教育和推广部分紧密结合在一起，包括研究生课程中的新项目和内容，REU/K-12活动，如科学节展示，iGEM本科团队指导，生物材料艺术展，以及生物制造扩展研讨会，以培养来自不同背景的未来科学家和工程师，在高度交叉的生物制造和生物材料研究领域。研究的目标是采用生物矿化的生态学概念，并重新利用真菌-细菌相互作用的共生原理来模拟和设计木质纤维素-生物质衍生材料内的活微生物网络作为生物合成添加剂制造和材料性质的原位调节的墨水。该项目的研究目标包括：1)墨水开发：设计、建模和生产生物质衍生、微生物集成和可打印的生物材料原料，用于原位生物矿物和生物聚合物合成；2)添加剂生物制造：使用活的组件进行3D打印，以优化材料的性能并定制活的建筑材料和结构的结构；3)基准和优化：表征和优化3D生物打印材料和结构的性能，包括强度、热性能、自修复能力、耐火性和碳存储。我们最终将利用当地的原料扩大油墨生产，并扩大印刷适宜性和生物制造，以进行环境影响分析和技术经济评估。最主要的重点将是更好地了解如何设计3D打印物理-生物系统中的微生物-联合体和惰性组件之间的协同作用，以提高材料的性能。该项目将允许以新的方式开发微生物相互依赖和真菌-细菌相互作用，以分析它们在不同时间尺度和物理维度的工程空间中的栖息、繁殖、新陈代谢、相互作用和生物安全性。该项目还将使PI能够开发一种新的生物制造工艺，将3D打印与基于高度可扩展和可持续的生物质材料作为原料的定制材料设计的生物合成工艺无缝结合。因此，该项目将使新的战略能够扩大混合生物-人造系统的生物制造能力。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。