EFRI ELiS: Biosynthetic Additive Manufacturing of Living Building Materials

EFRI ELiS：活性建筑材料的生物合成增材制造

• 批准号: 2318057
• 负责人: Jinxing Li
• 金额: $ 200万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2027-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2318057&HistoricalAwards=false
• 关键词: EFRI; ELiS; Biosynthetic; Additive; Manufacturing

This award aims to integrate cutting-edge approaches in advanced manufacturing, synthetic biology, and materials science to transform microbe-engineered lignocellulosic biomass into a printable ink for biosynthetic additive manufacturing of 3D-printed living materials and structures for building applications. The integration of carefully designed microbial networks in printable lignocellulose inks will produce hierarchically structured organic-inorganic living composites with enhanced mechanical and thermal properties, increased carbon storage, self-healing capacity, and scalable and modular fabrication. The award’s vision is that the technical innovations will lead to positive social impacts through: fighting climate change with carbon-negative living materials; combating homelessness through smart and modular building materials production; and filling technical gaps in the nation's Biomanufacturing and Bioeconomy initiative. The research thrusts will be tightly coupled with comprehensive educational and outreach components, including new projects and contents in graduate courses, REU/K-12 activities such as Science Festival showcase, iGEM undergraduate team mentorships, living materials art exhibitions, and biomanufacturing outreach workshops to prepare future scientists and engineers from diverse backgrounds in the highly interdisciplinary research fields of biomanufacturing and living materials.The goal of the research is to adopt ecological concepts of biomineralization and repurpose the symbiotic principles of fungal-bacterial interactions to model and design living microbial networks within lignocellulosic-biomass-derived materials as ink for biosynthetic additive manufacturing and in situ modulation of the material's properties. The research objectives of this project include: 1) ink Development: Design, model, and produce biomass-derived, microbe-integrated, and printable biomaterials feedstock for in situ biomineral and biopolymer synthesis; 2) additive Biomanufacturing: 3D printing with living components to optimize the material's performance and customize the construction of living building materials and structures; 3) benchmarking and Optimization: characterize and optimize the performance of the 3D bioprinted materials and structures, including strength, thermal properties, self-healing capacities, fire resistance, and carbon storage. We will finally scale up ink production with local feedstock, and scale up the printability and biomanufacturing for Environmental Impact Analysis and Techno-Economic Assessment. The overarching focus will be on obtaining a better understanding of how the synergized interactions between microbe-consortia and inert components within the 3D-printed physical-biological system can be engineered to enhance the material's performance. The project will allow the exploitation of microbial interdependencies and fungal-bacteria interactions in novel ways to analyze their habitation, reproduction, metabolism, interaction, and biosafety in an engineered space across different time scales and physical dimensions. The project will also allow the PI to develop a new biomanufacturing process that seamlessly integrates 3D printing and the biosynthetic process for tailored materials design based on highly scalable and sustainable biomass materials as feedstock. Therefore, this project will enable novel strategies to magnify biomanufacturing capacity in hybrid living-manmade systems.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项旨在整合先进制造、合成生物学和材料科学领域的前沿方法，将微生物工程木质纤维素生物质转化为可打印的油墨，用于3d打印生物材料和建筑结构的生物合成添加剂制造。将精心设计的微生物网络集成到可打印的木质纤维素油墨中，将产生分层结构的有机-无机活复合材料，具有增强的机械和热性能，增加的碳储存，自修复能力，以及可扩展和模块化制造。该奖项的愿景是，技术创新将通过以下方式产生积极的社会影响：用负碳的生活材料应对气候变化；通过智能和模块化建筑材料生产解决无家可归问题；填补国家生物制造和生物经济计划的技术空白。研究重点将与全面的教育和推广组成部分紧密结合，包括研究生课程的新项目和内容，REU/K-12活动，如科学节展示，iGEM本科团队指导，生物材料艺术展览和生物制造推广研讨会，以培养来自不同背景的未来科学家和工程师在生物制造和生物材料的高度跨学科研究领域。该研究的目标是采用生物矿化的生态概念，并重新利用真菌-细菌相互作用的共生原理来模拟和设计木质纤维素-生物质衍生材料中的活微生物网络，作为生物合成添加剂制造和材料特性原位调制的墨水。该项目的研究目标包括：1)墨水开发：设计、建模和生产生物质衍生的、微生物集成的、可打印的生物材料原料，用于原位生物矿物和生物聚合物的合成；2)增材生物制造：利用有生命的部件进行3D打印，优化材料的性能，定制建造有生命的建筑材料和结构；3)基准测试和优化：表征和优化生物3D打印材料和结构的性能，包括强度、热性能、自修复能力、耐火性和碳储存。我们最终将扩大使用当地原料的油墨生产，并扩大印刷能力和生物制造，以进行环境影响分析和技术经济评估。总体重点将是更好地了解如何设计3d打印物理生物系统中微生物群落和惰性成分之间的协同相互作用，以提高材料的性能。该项目将允许利用微生物的相互依赖关系和真菌-细菌的相互作用，以新颖的方式分析它们在不同时间尺度和物理维度的工程空间中的居住、繁殖、代谢、相互作用和生物安全性。该项目还将允许PI开发一种新的生物制造工艺，无缝集成3D打印和生物合成工艺，以高度可扩展和可持续的生物质材料为原料进行定制材料设计。因此，该项目将为扩大生物-人造混合系统的生物制造能力提供新的策略。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。