CAREER: The interaction of deficits in cellular regulation and divergent parental environments to postzygotic isolation in hybridizing milkweeds (Asclepias)

职业生涯：细胞调节缺陷和不同亲本环境与杂交马利筋（Asclepias）合子后隔离的相互作用

• 批准号: 2238209
• 负责人: Carrie Olson-Manning
• 金额: $ 121.21万
• 依托单位: Augustana University Association
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-06-15 至 2028-05-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2238209&HistoricalAwards=false
• 关键词: CAREER; interaction; deficits; cellular; regulation

This project will study how cellular regulation affects the reproductive barriers between two milkweed species in different environments. Understanding this is important because it sheds light on the ways in which species adapt to their environments and how they interact with each other. The project will use field experiments, genomics, and metabolomics analyses to explore the effects of hybridization and genetic and environmental perturbation on gene networks and cellular metabolism. This project will contribute to our understanding of cellular mechanisms, genetic incompatibilities, and the ways in which species interact with each other in different environments. This knowledge will be valuable in predicting how climate change and human activities might affect the relationships between different species. The broader impacts include engaging K12 students in South Dakota in research experiences and providing educational opportunities for underrepresented students. Finally, the project will develop an undergraduate Bioinformatics curriculum that focuses on quantitative reasoning and programming skills. This project will investigate how intrinsic and extrinsic reproductive barriers contribute to species isolation by examining the cellular regulatory dysfunction in hybrids of two milkweeds, Asclepias syriaca and A. speciosa. The study will focus on identifying the cellular regulatory networks and stress-response metabolism of each species in parental environments and characterizing the diversity of hybrid cellular expression and its impact on fitness. The research will be conducted through RNA-sequencing and quantification of stress metabolites in root and leaf tissue. The broader impacts are integrated into the research with experiments in common gardens at K12 schools across South Dakota to quantify the relative contributions of pre- and postzygotic barriers to reproductive isolation. Further integration of the scientific and educational aims will include the development of an undergraduate Bioinformatics curriculum that focuses on quantitative reasoning, modeling, and simulation, and applying the process of science via programming in R. This research will contribute to understanding gene interactions and the types of incompatibilities that arise during species isolation, which will become increasingly important as climate change and human disturbances shift species ranges and bring previously isolated species into contact.This project is jointly funded by the Integrative Ecological Physiology program (IEP) in Integrative Organismal Systems, the Established Program to Stimulate Competitive Research (EPSCoR), and the Evolutionary Processes (EP) program in the Division of Environmental Biology.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该项目将研究细胞调节如何影响不同环境中两个乳草物种之间的生殖屏障。理解这一点很重要，因为它阐明了物种适应其环境以及它们如何相互作用的方式。该项目将使用现场实验，基因组学和代谢组学分析来探索杂交，遗传和环境扰动对基因网络和细胞代谢的影响。该项目将有助于我们对细胞机制，遗传不相容性以及物种在不同环境中相互作用的方式的理解。这些知识对于预测气候变化和人类活动如何影响不同物种之间的关系将是有价值的。更广泛的影响包括让南达科他州的K12学生参与研究经验，并为代表性不足的学生提供教育机会。最后，该项目将开发本科生的生物信息学课程，重点介绍定量推理和编程技能。该项目将通过检查两种乳草，asclepias inriarca and A. speciosa的细胞调节功能障碍，研究内在和外在的生殖屏障如何促进物种隔离。该研究将着重于识别父母环境中每个物种的细胞调节网络和应力反应代谢，并表征杂交细胞表达的多样性及其对适应性的影响。该研究将通过对根和叶组织中应力代谢产物的RNA测序和定量进行。在南达科他州K12学校的普通花园中进行的实验将更广泛的影响纳入了研究中，以量化生殖隔离前后的同进壁垒的相对贡献。科学和教育目的的进一步整合将包括开发本科生物信息信息学课程，该课程的重点是定量推理，建模和模拟，以及通过编程通过R编程应用R。这项研究将有助于理解基因相互作用以及在隔离过程中会导致种类变化的物种的类型，这些物种会导致隔离和人性化的变化，这些物种会变得隔离，这些物种会导致风险变化，这些物种会变化，这些变化是在隔离的情况下，这些疾病的变化是在隔离的情况下，该物种的变化是造成的。该项目由综合有机体系统中的综合生态生理计划（IEP）共同资助，刺激竞争研究的既定计划（EPSCOR）以及环境生物学部的进化过程（EP）计划（EP）计划（EP）奖，该奖项反映了NSF的法定任务，并通过评估商标来反映出支持者的支持者，并已被评估范围。