EAGER: Hybridization and metabolic dysfunction in milkweeds

EAGER：马利筋的杂交和代谢功能障碍

• 批准号: 2017485
• 负责人: Carrie Olson-Manning
• 金额: $ 23.15万
• 依托单位: Augustana University Association
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-01 至 2022-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2017485&HistoricalAwards=false
• 关键词: EAGER; Hybridization; metabolic; dysfunction; milkweeds

This research aims to understand how biochemical processes and pathways in the cell are disrupted when mating occurs across species. Two milkweed species, the common milkweed and the showy milkweed, form hybrids across a wide region of the central United States. The research will determine the extent of natural hybridization between these species and identify the cellular pathways that differ between species. This information will allow identification of cellular pathways that are disrupted when hybridization occurs. This work will shed light on both the organization of biochemical pathways and the mechanisms that keep species reproductively isolated. Understanding the effects of hybridization will become increasingly important as climate change and human disturbance cause species ranges to shift and bring previously isolated species into contact. The results of the project will be integrated into undergraduate courses to strengthen bioinformatics and introductory biology courses with a focus on quantitative reasoning, modeling, and simulations.This research addresses a fundamental gap in our understanding of metabolic pathways and how the metabolome changes during speciation. When species evolve in isolation and come back into contact, the hybrids they form often display variation outside of the parental means (transgressive segregation). By studying natural and hand-pollinated hybrids along with the parental species, the researchers will uncover the changes to the genetic architecture that occur as species diverge. The researchers will use (1) genotype-by-sequencing to reconstruct the history of hybridization across the region of sympatry and (2) a comprehensive global metabolomics study to identify how pathways are disrupted following hybridization. These data will identify where pathway changes are likely to accumulate and identify new metabolites of interest. This project is jointly funded by the Evolutionary Processes Cluster in the Division of Environmental Biology and the Established Program to Stimulate Competitive Research (EPSCoR).This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这项研究旨在了解当物种之间发生交配时，细胞中的生化过程和途径如何破坏。两种乳草种，共同的乳草和艳丽的乳草，在美国中部广泛地区形成杂种。该研究将确定这些物种之间自然杂交的程度，并确定物种之间不同的细胞途径。该信息将允许识别发生杂交时被破坏的细胞途径。这项工作将阐明生化途径的组织和使物种生殖隔离的机制。随着气候变化和人类干扰导致物种范围的转移并将以前孤立的物种接触到，了解杂交的影响将变得越来越重要。该项目的结果将集成到本科课程中，以增强生物信息学和入门生物学课程，重点关注定量推理，建模和仿真。这项研究涉及我们对代谢途径的理解的根本差距，以及在物种物种过程中如何变化的代谢途径。当物种孤立地进化并恢复接触时，它们形成的杂种通常在父母平均值之外显示出变化（侵犯隔离）。通过研究天然和手工授粉的杂种以及父母物种，研究人员将发现随着物种差异而发生的遗传结构的变化。研究人员将使用（1）逐个基因型来重建整个杂志区域的杂交历史，以及（2）一项全面的全球代谢组学研究来确定杂交后如何破坏途径。这些数据将确定途径变化可能积累并确定感兴趣的新代谢物。该项目由环境生物学和既定计划的既定计划（EPSCOR）的进化过程集群共同资助。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛影响的审查标准通过评估来进行评估的。

项目成果

Analysis of the leaf metabolome in Arabidopsis thaliana mutation accumulation lines reveals association of metabolic disruption and fitness consequence

• DOI: 10.1007/s10682-022-10210-8
• 发表时间: 2022-09-10
• 期刊: EVOLUTIONARY ECOLOGY
• 影响因子: 1.9
• 作者: Kreutzmann，Sydney;Pompa，Elizabeth;Olson-Manning，Carrie F.
• 通讯作者: Olson-Manning，Carrie F.

Ecological factors influence balancing selection on leaf chemical profiles of a wildflower.

• DOI: 10.1038/s41559-021-01486-0
• 发表时间: 2021-08
• 期刊: Nature ecology & evolution
• 影响因子: 16.8
• 作者: Carley LN;Mojica JP;Wang B;Chen CY;Lin YP;Prasad KVSK;Chan E;Hsu CW;Keith R;Nuñez CL;Olson-Manning CF;Rushworth CA;Wagner MR;Wang J;Yeh PM;Reichelt M;Ghattas K;Gershenzon J;Lee CR;Mitchell-Olds T
• 通讯作者: Mitchell-Olds T