Collaborative Research: PurSUiT: A Backbone for the Peracarida

合作研究：PurSUiT：Peracarida 的支柱

• 批准号: 2321307
• 负责人: Regina Wetzer
• 金额: $ 69.77万
• 依托单位: Los Angeles County Museum of Natural History Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2024
• 资助国家: 美国
• 起止时间: 2024-03-15 至 2028-02-29
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2321307&HistoricalAwards=false
• 关键词: Collaborative; Research; PurSUiT; Backbone; Peracarida

Peracarids are an extremely large and diverse group of crustacean animals that are found everywhere on Earth, from deserts to the deepest ocean trenches. The most familiar peracarids are the terrestrial isopods, commonly known as roly polies, that are frequently found under rocks or plant pots. There are deep sea and freshwater versions of roly polies. They come in the most amazing body shapes and sizes - peracarids do just about everything except fly. Despite being found everywhere, very little is known about the relationships within Peracarida, limiting understanding of how the group came to be so species-rich and variable. The central goal of this project is to resolve the relationships within Peracarida. At least 30 undergraduate students, three graduate students, and a postdoctoral researcher will be trained in molecular laboratory techniques, bioinformatics, peracarid taxonomy, identification and description of new species, data management, and career development. Three workshops will be held to train 31 graduate students and early career professionals in peracarid systematics, collection, identification, preservation, bioinformatics, and data management. Project outreach will include social media, outreach to schools in diverse districts (Anchorage, Los Angeles, and western Alabama), museum exhibits, and bilingual (Spanish/English) outreach in traditional broadcast media and a short documentary film.The central goal of this project is to provide a robust phylogenetic framework for Peracarida based on broad taxon sampling. Currently, no such framework exists, which impedes research into important questions raised by the extreme diversity displayed across peracarid morphology, ecology, systematics and life history. The project will use a target capture phylogenomic approach with probe design informed by three new genomes and 50 new transcriptomes, as well as publicly available transcriptomes and genomes. Biodiversity across the order will be assessed using an integrative taxonomic approach combining DNA barcoding of COI, 16S and 18S and traditional morphological approaches. Beyond the immediate scope of the current proposal, this work is expected to catalyze research across Peracarida by providing a phylogenetic framework and a comprehensive set of phylogenomic tools specialized for Peracarida.This project is jointly funded by the Division of Environmental Biology and the Established Program to Stimulate Competitive Research (EPSCoR).This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

Peracarids是一种极其庞大和多样化的甲壳类动物，从沙漠到最深的海洋海沟，在地球上随处可见。最常见的附肢动物是陆生等足目动物，通常被称为roly polies，它们经常出现在岩石或植物盆栽下。有深海版和淡水版的滚滚鱼群。它们有着最令人惊叹的体型和体型--爬行动物几乎可以做任何事情，除了飞行。尽管随处可见，但人们对Peracarida内部的关系知之甚少，这限制了人们对该群体如何变得如此物种丰富和可变的理解。该项目的中心目标是解决佩拉卡里达内部的关系。至少30名本科生、3名研究生和1名博士后研究员将接受分子实验室技术、生物信息学、干旱带分类学、新物种识别和描述、数据管理和职业发展方面的培训。将举办三期讲习班，培训31名研究生和职业生涯初期的专业人员，内容涉及旱地系统学、收集、鉴定、保存、生物信息学和数据管理。项目外展将包括社交媒体、不同地区(安克雷奇、洛杉矶和阿拉巴马州西部)的学校外展、博物馆展品、传统广播媒体的双语(西班牙语/英语)外展和一部简短的纪录片。该项目的中心目标是基于广泛的分类群采样为Peracarida提供一个强大的系统发育框架。目前，还没有这样的框架，这阻碍了对跨干旱形态、生态学、系统学和生活史表现出的极端多样性所提出的重要问题的研究。该项目将使用目标捕获系统基因组学方法，通过三个新基因组和50个新转录本以及公开提供的转录本和基因组进行探针设计。整个目的生物多样性将使用一种综合的分类学方法进行评估，该方法结合了COI、16S和18S的DNA条码和传统的形态方法。除了当前提议的直接范围之外，这项工作预计将通过提供一个系统发育框架和一套专门针对Peracarida的全面的系统发育学工具来促进整个Peracarida的研究。该项目由环境生物学部门和既定的刺激竞争研究计划(EPSCoR)联合资助。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。