Collaborative Research: PurSUiT: A Backbone for the Peracarida

合作研究：PurSUiT：Peracarida 的支柱

• 批准号: 2321308
• 负责人: Kevin Kocot
• 金额: $ 84.08万
• 依托单位: University of Alabama Tuscaloosa
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2024
• 资助国家: 美国
• 起止时间: 2024-03-15 至 2028-02-29
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2321308&HistoricalAwards=false
• 关键词: Collaborative; Research; PurSUiT; Backbone; Peracarida

Peracarids are an extremely large and diverse group of crustacean animals that are found everywhere on Earth, from deserts to the deepest ocean trenches. The most familiar peracarids are the terrestrial isopods, commonly known as roly polies, that are frequently found under rocks or plant pots. There are deep sea and freshwater versions of roly polies. They come in the most amazing body shapes and sizes - peracarids do just about everything except fly. Despite being found everywhere, very little is known about the relationships within Peracarida, limiting understanding of how the group came to be so species-rich and variable. The central goal of this project is to resolve the relationships within Peracarida. At least 30 undergraduate students, three graduate students, and a postdoctoral researcher will be trained in molecular laboratory techniques, bioinformatics, peracarid taxonomy, identification and description of new species, data management, and career development. Three workshops will be held to train 31 graduate students and early career professionals in peracarid systematics, collection, identification, preservation, bioinformatics, and data management. Project outreach will include social media, outreach to schools in diverse districts (Anchorage, Los Angeles, and western Alabama), museum exhibits, and bilingual (Spanish/English) outreach in traditional broadcast media and a short documentary film.The central goal of this project is to provide a robust phylogenetic framework for Peracarida based on broad taxon sampling. Currently, no such framework exists, which impedes research into important questions raised by the extreme diversity displayed across peracarid morphology, ecology, systematics and life history. The project will use a target capture phylogenomic approach with probe design informed by three new genomes and 50 new transcriptomes, as well as publicly available transcriptomes and genomes. Biodiversity across the order will be assessed using an integrative taxonomic approach combining DNA barcoding of COI, 16S and 18S and traditional morphological approaches. Beyond the immediate scope of the current proposal, this work is expected to catalyze research across Peracarida by providing a phylogenetic framework and a comprehensive set of phylogenomic tools specialized for Peracarida.This project is jointly funded by the Division of Environmental Biology and the Established Program to Stimulate Competitive Research (EPSCoR).This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

paracarids是一群极为大而多样的甲壳类动物，在地球上到处都有，从沙漠到最深的海沟。最熟悉的多核酸是陆生异脚架，通常称为roly派，它们经常在岩石或种植盆中发现。有深海和淡水版本的ROLY派。它们具有最惊人的身体形状和尺寸 - Peracarids除了飞蝇外几乎所有的事情。尽管到处都发现，但对佩拉卡里达（Peracarida）内部的关系知之甚少，这限制了对该群体如何变得如此丰富和可变的理解。该项目的核心目标是解决Peracarida内部的关系。至少有30名本科生，三名研究生和一名博士后研究人员将接受分子实验室技术，生物信息学，多核分类法，新物种的识别和描述，数据管理和职业发展的培训。将举行三个研讨会，以培训31名研究生和早期职业专业人员，以培训多生性系统学，收集，识别，保存，生物信息学和数据管理。计划外展活动将包括社交媒体，在传统广播媒体中的不同地区（安克雷奇，洛杉矶和阿拉巴马州西部）的学校（安克雷奇，洛杉矶和西部阿拉巴马州）的学校以及双语（西班牙语/英语）外展活动以及简短的纪录片中。目前，尚无这样的框架，这阻碍了对多种多样性，生态学，系统学和生活历史所显示的极端多样性提出的重要问题。该项目将使用目标捕获系统基因组方法，其中包括三个新基因组和50个新转录组以及公开可用的转录组和基因组的探针设计。整个订单的生物多样性将使用综合分类方法进行评估，该方法结合了COI，16S和18S的DNA条形码以及传统的形态学方法。除了当前提议的直接范围之外，这项工作有望通过提供系统发育框架和一组专门用于Peracarida专门的系统发育框架和一套全面的系统发育框架来催化整个研究。该项目由环境生物学部共同资助，由环境生物学司共同资助。基金会的智力优点和更广泛的影响评论标准。