Collaborative Research: PurSUiT: A Backbone for the Peracarida

Peracarids是一种非常庞大和多样化的甲壳动物，在地球上的任何地方都可以找到，从沙漠到最深的海沟。最常见的是陆地等足类动物，通常被称为roly polies，经常在岩石或花盆下发现。有深海和淡水版本的roly polies。它们有着最令人惊奇的身体形状和大小--除了飞，它们几乎什么都能做。尽管到处都可以找到，但对Peracarida内部的关系知之甚少，限制了对该群体如何变得如此物种丰富和可变的理解。该项目的中心目标是解决Peracarida内部的关系。至少有30名本科生，三名研究生和一名博士后研究员将接受分子实验室技术，生物信息学，peracarid分类学，新物种鉴定和描述，数据管理和职业发展的培训。将举办三个讲习班，培训31名研究生和早期职业专业人员在peracarid系统学，收集，识别，保存，生物信息学和数据管理。项目外展将包括社会媒体，外展到不同地区（安克雷奇，洛杉矶和亚拉巴马西部）的学校，博物馆展览，并在传统的广播媒体和一个简短的纪录片双语（西班牙语/英语）的推广。目前，没有这样的框架存在，这阻碍了研究所提出的重要问题的极端多样性显示在peracarid形态学，生态学，系统学和生活史。该项目将使用靶向捕获基因组方法，探针设计由三个新的基因组和50个新的转录组以及公开可用的转录组和基因组提供信息。整个秩序的生物多样性将使用综合分类方法相结合的COI，16 S和18 S的DNA条形码和传统的形态学方法进行评估。在当前提案的直接范围之外，这项工作有望通过提供一个系统发育框架和一套全面的针对桡足类的基因组学工具来促进整个桡足类的研究。2该项目由环境生物学部和刺激竞争研究的既定计划（EPSCoR）共同资助该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

Peracarids are an extremely large and diverse group of crustacean animals that are found everywhere on Earth, from deserts to the deepest ocean trenches. The most familiar peracarids are the terrestrial isopods, commonly known as roly polies, that are frequently found under rocks or plant pots. There are deep sea and freshwater versions of roly polies. They come in the most amazing body shapes and sizes - peracarids do just about everything except fly. Despite being found everywhere, very little is known about the relationships within Peracarida, limiting understanding of how the group came to be so species-rich and variable. The central goal of this project is to resolve the relationships within Peracarida. At least 30 undergraduate students, three graduate students, and a postdoctoral researcher will be trained in molecular laboratory techniques, bioinformatics, peracarid taxonomy, identification and description of new species, data management, and career development. Three workshops will be held to train 31 graduate students and early career professionals in peracarid systematics, collection, identification, preservation, bioinformatics, and data management. Project outreach will include social media, outreach to schools in diverse districts (Anchorage, Los Angeles, and western Alabama), museum exhibits, and bilingual (Spanish/English) outreach in traditional broadcast media and a short documentary film.The central goal of this project is to provide a robust phylogenetic framework for Peracarida based on broad taxon sampling. Currently, no such framework exists, which impedes research into important questions raised by the extreme diversity displayed across peracarid morphology, ecology, systematics and life history. The project will use a target capture phylogenomic approach with probe design informed by three new genomes and 50 new transcriptomes, as well as publicly available transcriptomes and genomes. Biodiversity across the order will be assessed using an integrative taxonomic approach combining DNA barcoding of COI, 16S and 18S and traditional morphological approaches. Beyond the immediate scope of the current proposal, this work is expected to catalyze research across Peracarida by providing a phylogenetic framework and a comprehensive set of phylogenomic tools specialized for Peracarida.This project is jointly funded by the Division of Environmental Biology and the Established Program to Stimulate Competitive Research (EPSCoR).This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.