CAREER: Unraveling the Developmental Processes Underlying the Twining Motion of Vines

攀援的藤蔓在森林树冠层中穿梭，缠绕着宿主树木，在树冠层的顶部寻找光线。攀缘藤是森林生态系统的重要组成部分，它们经常在竞争中击败并杀死森林树木。此外，这些植物的大小和丰富度正在增加，以应对森林的破碎化和气候变化。然而，我们仍然缺乏对这些植物如何执行其戏剧性的蛇形缠绕运动的基本了解。在这个CAREER奖项中，将调查缠绕的发展基础。将研究细胞、组织、激素和分子生物学如何相互作用来构建缠绕藤，目的是揭示藤与直立植物不同的根本原因。为了提高公众和专业素养的植物是如何构建的-植物解剖学领域-PI将推出一个教育视频系列教学植物解剖学与现场解剖和动画。这个视频系列将免费提供给新手，并作为专业科学家和教育工作者的入门和教学工具。在这个职业奖中，调查人员试图解决以下问题-“葡萄藤是如何攀爬的？”- 通过利用多尺度整合方法将基因表达、细胞壁构建和激素调节与有机体形式联系起来，来解释产生缠绕藤蔓的发育过程。以普通菜豆、菜豆（Phaseolus vulgaris L.）（Fabaceae）作为模式种，本研究的目的是：（1）表征支撑从缠绕植物到匍匐藤本植物再到直立灌木的连续性习性的发育基础;（2）阐明植物激素如何决定植物习性;（3）测试缠绕植物中凝胶纤维的功能作用。目前，在美国，分子生物学和植物解剖学之间的培训和教学存在着显著的不平衡，因此阻碍了植物科学的进步。这项工作的更广泛的影响目标是生成一个动画教育植物解剖视频系列。该资源将作为一个免费的资源，以提高公众对植物学的认识，同时为科学界提供严重缺乏的专业知识。教育目标是：（1）通过调查植物界的需求，找出教学方面的差距，以便进行有针对性的干预;（2）编写、制作和传播植物解剖学教育录像系列“建造一个植物”;以及（3）将植物解剖学学习成果融入本科课程。该奖项由BIO-IOS-Developmental Systems和BIO-IOS-Developmental Systems共同资助。生理机制和生物力学计划。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

Climbing vines weave through the forest canopy wrapping themselves around host trees in search of light at the top of the canopy. Climbing vines are a critical part of forest ecosystems, where they frequently outcompete and kill forest trees. Moreover, these plants are increasing in size and abundance in response to forest fragmentation and climate change. However, we still lack a basic understanding of precisely how these plants perform their dramatic snake-like twining motions. In this CAREER award, the developmental basis of twining will be investigated. How cells, tissues, hormones, and molecular biology interact to build a twining vine will be investigated, with the aim of revealing the fundamental reasons that vines are distinct from erect plants. To enhance public and professional literacy of how plants are constructed – the field of plant anatomy – the PI will launch an educational video series teaching plant anatomy with live dissections and animations. This video series will be made freely available to engage novice, and as a primer and teaching tool for professional scientists and educators.In this CAREER award, the investigator seeks to address the following question – “how do vines climb?” – by leveraging a multiscale integrative approach linking gene expression, cell wall construction, and hormone regulation to organismal form to explain the developmental processes that generate twining vines. Using common bean, Phaseolus vulgaris L. (Fabaceae), as the model species, the research aims of this proposal are to: (1) Characterize the developmental basis underpinning the continuum of habits, from twiners, to prostrate vine, to erect shrub; (2) Elucidate how phytohormones determine plant habit; and (3) Test the functional role of gelatinous fibers in twiners. At present, there is a significant imbalance in training and pedagogy between molecular biology and plant anatomy in the United States, therefore hindering advancements in the plant sciences. The broader impacts aim of this work is to generate an animated educational Plant Anatomy video series. This resource will serve as a freely available resource to advance public literacy of botany while providing critically absent expertise for the scientific community. The educational aims are to: (1) Identify gaps in the pedagogy for targeted intervention through surveying the needs of the botanical community; (2) Write, produce, and disseminate a plant anatomy educational videos series “Build a Plant”; and (3) Integrate plant anatomy learning outcomes in undergraduate courses.This award is co-funded by BIO-IOS-Developmental Systems and the BIO-IOS-Physiological Mechanisms and Biomechanics program.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.