Collaborative Research: Electromagnet-integrated optical microscope stage with biocompatible magnetogel for investigating mechanobiology in 2D and 3D

合作研究：带有生物相容性磁凝胶的电磁集成光学显微镜载物台，用于研究 2D 和 3D 力学生物学

• 批准号: 2414158
• 负责人: Andrea Armani
• 金额: $ 79.21万
• 依托单位: ELLISON INSTITUTE, LLC
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-11-01 至 2025-09-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2414158&HistoricalAwards=false
• 关键词: Collaborative; Research; Electromagnet; integrated; optical

An award is made to the University of Southern California in collaboration with Brown University to develop an instrument that can be used to investigate how mechanical forces can control or influence biological systems. Through the research activities, the award will provide training to undergraduate students, PhD students, and post-doctoral scholars. Notably, collaborating with the newly started NSF Advanced Technological Education Center located at Pasadena City College, will enable the hosting of two undergraduate community college students, directly incorporating undergraduates into the research activities. These students will be mentored by the PhD students and post-doctoral scholar who will be involved in this research effort. The finding will be disseminated through freely accessible online repositories such as the NIH 3D Print Exchange and GitHub and through scholarly publications and conference presentations. To increase the scientific literacy of the general public, social media will be leveraged for broader dissemination of the research findings and scientific life.It is well-established that chemical exposure can modify a biological system, changing the behavior of cells and tissue. Evidence is emerging that physical forces can play a similar role, and in some cases, these physical effects can combine with chemical stimuli to amplify or to eliminate a biological response. Changes in the mechanical properties of surfaces in contact with cells and tissues also modulate biological activity. Therefore, it is critical to understand the role of mechanical forces in biological processes. However, only a few tools can accurately study this complex behavior in limited settings. This award will support the development and validation of a more broadly applicable instrument that will overcome this cross-cutting hurdle. The technology will be compatible with a standard fluorescence microscope, enabling simultaneous imaging and manipulation of the physical properties of the biological system. To accomplish this goal, two new synergistic technologies will be developed. To demonstrate relevance to current and future NSF BIO Directorate programs, a pair of proof-of-concept measurements in neuroscience and in bacterial research will be performed.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

与布朗大学合作，向南加州大学颁发了奖励，以开发一种可以用来调查机械力如何控制或影响生物系统的工具。通过研究活动，该奖项将为本科生，博士生和博士后学者提供培训。值得注意的是，与位于帕萨迪纳市学院的新开设的NSF高级技术教育中心合作，将使两名本科生学院的学生能够直接将本科生纳入研究活动。这些学生将受到博士生和博士后学者的指导，他们将参与这项研究工作。该发现将通过自由访问的在线存储库（例如NIH 3D打印交易所和GitHub）以及学术出版物和会议演讲来传播。为了提高公众的科学素养，社交媒体将被利用，以更广泛地传播研究发现和科学生活，这是确定的，化学暴露可以改变生物系统，改变细胞和组织的行为。有证据表明，物理力可以发挥相似的作用，在某些情况下，这些物理作用可以与化学刺激结合起来，以扩增或消除生物学反应。与细胞和组织接触的表面机械性能的变化也调节生物学活性。因此，了解机械力在生物过程中的作用至关重要。但是，只有少数工具可以在有限的设置中准确研究这种复杂的行为。该奖项将支持一种更广泛适用的工具的开发和验证，该工具将克服这一跨切障碍。该技术将与标准的荧光显微镜兼容，从而同时对生物系统的物理特性进行操纵。为了实现这一目标，将开发两种新的协同技术。为了证明与当前和未来的NSF生物局计划的相关性，将进行神经科学和细菌研究中的概念概念证明测量值。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛影响的审查标准来通过评估来通过评估来支持的。