Structure-function analysis of the auxin transport regulatory D6 PROTEIN KINASE in Arabidopsis thaliana

拟南芥生长素转运调节 D6 蛋白激酶的结构-功能分析

• 批准号: 259142660
• 负责人: Professor Dr. Claus Schwechheimer
• 金额: --
• 依托单位: Lehrstuhl für Systembiologie der Pflanzen
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/259142660?language=en
• 关键词: Structure; function; analysis; auxin; transport

The hormone auxin controls many decisions in plant development as well as all tropic responses. The correct distribution of auxin within the plant is achieved by a system of import and export carriers. D6 PROTEIN KINASE (D6PK) is important for the activation of PIN-FORMED (PIN) auxin exporters and mutants of D6PK have defects in lateral root formation, shoot differentiation, and various tropic growth responses. D6PK as PINs are polarly distributed in plant cells and thereby direct auxin transport streams in the plant. Understanding the trafficking mechanisms underlying D6PK and PIN transport is therefore pivotal to understand plant development and tropisms. Here, we aim at understanding the molecular mechanisms that target D6PK to the plasma membrane and that regulate D6PK plasma membrane association and kinase activity. We showed that D6PK associates with phospholipids in the plasma membrane through a positively charged lysine-motif and now will address the role of repeats containing cysteines for membrane binding and kinase activity. To place these structure-function analysis in a broader context, we also aim at determining the crystal structure of D6PK and to examine the behaviour of D6PK in planta in selected genetic backgrounds.

生长素激素控制着植物发育中的许多决定以及所有的向性反应。植物生长素在植物中的正确分布是通过一个进出口载体系统实现的。D 6蛋白激酶（D 6PK）对于PIN形成的（PIN）生长素输出蛋白的激活是重要的，并且D 6PK的突变体在侧根形成、芽分化和各种向性生长反应中具有缺陷。D 6PK作为PIN极性分布在植物细胞中，从而引导植物中的生长素运输流。因此，了解D 6PK和PIN运输的运输机制对于了解植物发育和向性至关重要。在这里，我们的目的是了解靶向D 6PK的质膜和调节D 6PK质膜协会和激酶活性的分子机制。我们发现D 6PK通过带正电荷的赖氨酸基序与质膜中的磷脂相关联，现在将解决含有半胱氨酸的重复序列对膜结合和激酶活性的作用。为了将这些结构-功能分析置于更广泛的背景下，我们还旨在确定D 6PK的晶体结构，并在选定的遗传背景下检查D 6PK在植物中的行为。

项目成果

Auxin does not inhibit endocytosis of PIN1 and PIN2 auxin efflux carriers.

生长素不抑制 PIN1 和 PIN2 生长素外排载体的内吞作用

• DOI: 10.1093/plphys/kiab132
• 发表时间: 2021
• 期刊: Plant physiology
• 影响因子: 7.4
• 作者: Schwechheimer;Yalovsky;Zarsky
• 通讯作者: Zarsky

Roles of AGCVIII Kinases in the Hypocotyl Phototropism of Arabidopsis Seedlings

• DOI: 10.1093/pcp/pcy048
• 发表时间: 2018-05-01
• 期刊: PLANT AND CELL PHYSIOLOGY
• 影响因子: 4.9
• 作者: Haga, Ken;Frank, Lena;Sakai, Tatsuya
• 通讯作者: Sakai, Tatsuya

Dynamic PIN-FORMED auxin efflux carrier phosphorylation at the plasma membrane controls auxin efflux-dependent growth

• DOI: 10.1073/pnas.1614380114
• 发表时间: 2017-01-31
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Weller, Benjamin;Zourelidou, Melina;Schwechheimer, Claus
• 通讯作者: Schwechheimer, Claus

A molecular rheostat adjusts auxin flux to promote root protophloem differentiation

• DOI: 10.1038/s41586-018-0186-z
• 发表时间: 2018-06-14
• 期刊: NATURE
• 影响因子: 64.8
• 作者: Marhava, P.;Bassukas, A. E. L.;Hardtke, C. S.
• 通讯作者: Hardtke, C. S.

Arabidopsis Protein Kinase D6PKL3 Is Involved in the Formation of Distinct Plasma Membrane Aperture Domains on the Pollen Surface

• DOI: 10.1105/tpc.18.00442
• 发表时间: 2018-09-01
• 期刊: PLANT CELL
• 影响因子: 11.6
• 作者: Lee, Byung Ha;Weber, Zachary T.;Dobritsa, Anna A.
• 通讯作者: Dobritsa, Anna A.