MAP kinase regulation of PIN-FORMED auxin transporters

MAP 激酶对 PIN 形成的生长素转运蛋白的调节

• 批准号: 446615548
• 负责人: Professor Dr. Claus Schwechheimer
• 金额: --
• 依托单位: Lehrstuhl für Systembiologie der Pflanzen
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/446615548?language=en
• 关键词: MAP; kinase; regulation; PIN; FORMED

Auxin is a mobile plant hormone that is directionally transported from cell-to-cell through the activity of polarly distributed PIN auxin efflux transporters. Understanding auxin transport activity and directional transport is of great importance for the understanding of fundamental processes in plant development. In recent years, we and others have elucidated the role of PIN phosphorylation at conserved serine (S) residues for the regulation of PIN activity, plasma membrane distribution and polarity. Strikingly, three PIN serine residues required for PIN activation and polarity control, S1 – S3, reside in close proximity to hypothesized MAP kinase-targeted threonine phosphosites, T1 – T3, in the highly conserved T1PRXS1 – T3PRXS3 motifs. The role of T1 – T3 phosphosite phosphorylation, as well as that of a co-regulated S337 MAP kinase target site, for PIN function has not been elucidated as yet. Here, I propose to use immunostaining with phosphosite specific antibodies and quantitative phosphoproteomics to study the distribution and abundance of T phosphosite phosphorylation, alone and in conjunction with S phosphosite phosphorylation. Further, I propose to study the abundance of these phosphorylations in MAP signalling mutants to identify in planta MAP signalling cascades required for T phosphorylation. Finally, I propose to examine the role of T phosphosite phosphorylation for PIN activation, polarity control and other cell biological functions using a combination of genetics, cell biology and physiology, in isolation and together with S1 – S3 phosphorylation.

生长素是一种移动的植物激素，通过极性分布的PIN生长素外排转运蛋白的活性在细胞间定向转运。了解生长素的运输活动和定向运输对于了解植物发育的基本过程非常重要。近年来，我们和其他人已经阐明了PIN磷酸化在保守的丝氨酸（S）残基上对PIN活性、质膜分布和极性的调节作用。引人注目的是，PIN激活和极性控制所需的三个PIN丝氨酸残基，S1 - S3，位于高度保守的T1 PRXS 1-T3 PRXS 3基序中的假设MAP激酶靶向苏氨酸磷酸化位点T1 - T3附近。T1 - T3磷酸化位点的磷酸化，以及一个共同调节的S337 MAP激酶靶位点的磷酸化对PIN功能的作用尚未阐明。在这里，我建议使用免疫染色与磷酸化特异性抗体和定量磷酸蛋白质组学研究的分布和丰富的T磷酸化，单独和与S磷酸化。此外，我建议研究这些磷酸化的MAP信号突变体的丰度，以确定在植物MAP信号级联所需的T磷酸化。最后，我建议研究的作用T磷酸化PIN激活，极性控制和其他细胞生物学功能，使用遗传学，细胞生物学和生理学的组合，在隔离，并与S1 - S3磷酸化。