Regulatory functions of LLM-domain B-GATA transcription factors in essential plant growth processes.

LLM 结构域 B-GATA 转录因子在植物生长过程中的调节功能。

• 批准号: 190473740
• 负责人: Professor Dr. Claus Schwechheimer
• 金额: --
• 依托单位: Lehrstuhl für Systembiologie der Pflanzen
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/190473740?language=en
• 关键词: Regulatory; functions; LLM; domain; GATA

GATAs are evolutionarily conserved transcription factors in eukaryotes. The GATA family is substantially larger in plants than in animals and fungi, but the knowledge about plant GATAs is still rather limited. We have shown that LLM-domain B-GATAs redundantly regulate chlorophyll biosynthesis and stomata formation in Arabidopsis thaliana. Thus, these GATAs control directly or indirectly photosynthetic efficiency, a fundamental process in plants and for life on earth. In dark-grown wild type seedlings, the effects of the GATAs on chlorophyll biosynthesis and stomata formation are suppressed but, also in the dark, they can be activated in mutants of the light-labile PHYTOCHROME INTERACTING FACTORs. Here, we propose to identify LLM-domain B-GATA target genes during light-induced chlorophyll biosynthesis and stomata formation, to determine the binding specificity of these B-GATAs and to characterize the regulation of selected target genes at the functional and genetic level. We further propose to identify the factor(s) that suppress the effects of the GATAs in the dark using biased and unbiased biochemical and genetic approaches. Taken together, this research will lead to an improved understanding of LLM-domain B-GATA function and contribute to the understanding of the regulation of essential processes in plant growth.

GATA是真核生物中进化上保守的转录因子。植物中的加塔家族比动物和真菌中的要大得多，但是关于植物GATA的知识仍然相当有限。我们已经证明LLM结构域B-GATA冗余地调节拟南芥的叶绿素生物合成和气孔形成。因此，这些GATA直接或间接地控制光合效率，这是植物和地球上生命的基本过程。在黑暗生长的野生型幼苗中，GATA对叶绿素生物合成和气孔形成的影响受到抑制，但在黑暗中，它们也可以在光不稳定的光敏色素相互作用因子的突变体中被激活。在这里，我们建议确定LLM结构域B-GATA靶基因在光诱导的叶绿素生物合成和气孔形成，以确定这些B-GATA的结合特异性，并在功能和遗传水平上表征所选靶基因的调控。我们进一步建议使用有偏见和无偏见的生物化学和遗传方法来确定在黑暗中抑制GATA效应的因素。总而言之，这项研究将加深对LLM结构域B-GATA功能的了解，并有助于了解植物生长中基本过程的调节。