Regulatory functions of LLM-domain B-GATA transcription factors in essential plant growth processes.

gata是真核生物中进化保守的转录因子。植物中的GATA家族比动物和真菌中的要大得多，但对植物GATA的认识仍然相当有限。我们已经证明llm结构域b - gata冗余调节拟南芥叶绿素生物合成和气孔形成。因此，这些GATAs直接或间接地控制着光合作用效率，这是植物和地球上生命的一个基本过程。在黑暗生长的野生型幼苗中，GATAs对叶绿素生物合成和气孔形成的影响受到抑制，但在黑暗中，它们可以在光不稳定的光敏色素相互作用因子突变体中被激活。在此，我们提出在光诱导叶绿素生物合成和气孔形成过程中鉴定llm结构域B-GATA靶基因，以确定这些B-GATA的结合特异性，并在功能和遗传水平上表征所选靶基因的调控。我们进一步建议使用有偏和无偏的生化和遗传方法来确定在黑暗中抑制GATAs作用的因素。综上所述，本研究将有助于提高对llm结构域B-GATA功能的理解，并有助于理解植物生长过程中重要过程的调控。

GATAs are evolutionarily conserved transcription factors in eukaryotes. The GATA family is substantially larger in plants than in animals and fungi, but the knowledge about plant GATAs is still rather limited. We have shown that LLM-domain B-GATAs redundantly regulate chlorophyll biosynthesis and stomata formation in Arabidopsis thaliana. Thus, these GATAs control directly or indirectly photosynthetic efficiency, a fundamental process in plants and for life on earth. In dark-grown wild type seedlings, the effects of the GATAs on chlorophyll biosynthesis and stomata formation are suppressed but, also in the dark, they can be activated in mutants of the light-labile PHYTOCHROME INTERACTING FACTORs. Here, we propose to identify LLM-domain B-GATA target genes during light-induced chlorophyll biosynthesis and stomata formation, to determine the binding specificity of these B-GATAs and to characterize the regulation of selected target genes at the functional and genetic level. We further propose to identify the factor(s) that suppress the effects of the GATAs in the dark using biased and unbiased biochemical and genetic approaches. Taken together, this research will lead to an improved understanding of LLM-domain B-GATA function and contribute to the understanding of the regulation of essential processes in plant growth.