Temperature-dependent control of flowering by the gibberellin pathway and interactions between DELLA proteins and APETALA1/VRN1 MADS-box factors.

赤霉素途径对开花的温度依赖性控制以及 DELLA 蛋白和 APETALA1/VRN1 MADS-box 因子之间的相互作用。

• 批准号: 196930989
• 负责人: Professor Dr. Claus Schwechheimer
• 金额: --
• 依托单位: Lehrstuhl für Systembiologie der Pflanzen
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/196930989?language=en
• 关键词: Temperature; dependent; control; flowering; gibberellin

Temperature is an important environmental cue controlling flowering time. In Arabidopsis, the experience of cold temperatures delays growth and flowering and, at the molecular level, cold results in the accumulation of DELLA proteins, major plant growth and flowering time repressors of the gibberellin (GA) signalling pathway. DELLA abundance responds rather instantly to changes in the temperature and the effects of DELLA accumulation can be suppressed by GA (treatments). We have previously shown that the Arabidopsis MADS-box transcription factor APETALA1 (AP1) is repressed by direct interactions with DELLAs. Furthermore, we have evidence that increased AP1 expression may be causative for the early flowering of two Arabidopsis accessions in cold temperature. Here, we would like to test the hypothesis that increased AP1 levels in these accessions outcompete the growth repressive effects of DELLAs in the cold. Second, we would like to test whether this AP1-DELLA regulatory module is conserved in cereal species. In barley and wheat, VERNALIZATION1 (VRN1) proteins, the closest homologues to Arabidopsis AP1, are major flowering time regulators. We therefore would like to examine whether barley and wheat VRN1 can also interact with the respective DELLAs and whether flowering time control in response to temperature and GA is dependent on this interaction.

温度是控制花期的重要环境线索。在拟南芥中，低温会延迟生长和开花，在分子水平上，寒冷会导致DELLA蛋白的积累，这是赤霉素（GA）信号通路的主要植物生长和开花时间抑制因子。DELLA丰度对温度变化的反应相当迅速，并且可以通过GA（处理）抑制DELLA积累的影响。我们之前已经证明拟南芥MADS-box转录因子aptala1 （AP1）通过与DELLAs的直接相互作用而受到抑制。此外，我们有证据表明AP1表达的增加可能是两个拟南芥材料在低温下提前开花的原因。在这里，我们想要验证这样一个假设，即在这些材料中AP1水平的增加胜过DELLAs在寒冷条件下的生长抑制作用。其次，我们想测试这个AP1-DELLA调控模块在谷类物种中是否保守。在大麦和小麦中，与拟南芥AP1最接近的同源蛋白VERNALIZATION1 （VRN1）是主要的开花时间调节因子。因此，我们想要研究大麦和小麦VRN1是否也可以与各自的DELLAs相互作用，以及开花时间对温度和GA的响应是否依赖于这种相互作用。