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Novel Non-Linear Spectroscopic Probes of the Primary Photoreactions of Photolyases and Cryptochromes

光裂合酶和隐花色素初级光反应的新型非线性光谱探针

基本信息

批准号：
260966011
负责人：
Professor Dr. Benjamin Fingerhut
金额：
--
依托单位：
Department Chemie
依托单位国家：
德国
项目类别：
Independent Junior Research Groups
财政年份：
2014
资助国家：
德国
起止时间：
2013-12-31 至 2019-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/260966011?language=en
关键词：
Novel Non Linear Spectroscopic Probes

项目摘要

The aim of this project is the development of novel simulation algorithms for state-of-the-art time resolved vibrational and optical spectroscopic techniques. Both techniques exploit different windows of observation to provide a real time view of ultrafast, conical intersection mediated photoreactions. The involved molecular motions can be directly observed by time- and frequency-resolved infrared or Raman techniques. For their simulation a hierarchy of algorithms will be developed which cover full quantum simulation protocols as benchmark for introduced semiclassical approximations to facilitate simulations of characteristic vibronic signatures of biological relevant systems. Multidimensional optical spectroscopic techniques monitor energy fluxes and interactions between chromophores. Novel simulation protocols based on a co-bosonization of fermionic multi-excitons will allow to predict innovative higher order signals which reveal many-body correlations in chromophore aggregates and make transport phenomena in the important UV spectral region accessible. The algorithmic developments will be used to uncover the mechanism of the complex primary photoreactions in the Photolyase/Cryptochrome flavoprotein superfamily. Time-resolved vibrational techniques will allow to predict real-time molecular probes of the photolesion reversion to resolve controversial mechanistic predictions of (6-4) photoproduct repair. Predicted multidimensional optical signals will be used to discriminate between the charge transfer pathways in Cryptochromes to develop a universal model of the primary steps of signal transduction. Novel multidimensional chirality induced signals have the potential to follow conformational changes upon light absorption in real time in order to provide a detailed picture of the conversion of electronic excitation into the mechanical motion required for signal transduction. The theoretical developments of the project have thus the potential to uncover spectroscopic signatures of the earliest mechanistic steps of biological important processes by relating the known protein structure to the light induced dynamics to provide a comprehensive picture of the protein function.

该项目的目的是为最先进的时间分辨振动和光学光谱技术开发新的模拟算法。这两种技术都利用不同的观察窗口，以提供超快、圆锥形交叉介导的光反应的真实的时间视图。所涉及的分子运动可以通过时间和频率分辨的红外或拉曼技术直接观察到。对于他们的模拟算法的层次结构将开发，其中包括完整的量子模拟协议作为基准引入半经典近似，以促进生物相关系统的特征振动签名的模拟。多维光学光谱技术监测能量通量和发色团之间的相互作用。基于费米子多激子的共玻色化的新模拟协议将允许预测创新的高阶信号，其揭示发色团聚集体中的多体相关性，并使重要的UV光谱区域中的传输现象变得容易。算法的发展将被用来揭示复杂的初级光反应的Photolyase/隐花色素黄素蛋白超家族的机制。时间分辨振动技术将允许预测光损伤回复的实时分子探针，以解决（6-4）光产物修复的有争议的机理预测。预测的多维光信号将用于区分隐花色素中的电荷转移途径，以开发信号转导的主要步骤的通用模型。新颖的多维手性诱导的信号具有在真实的时间内跟踪光吸收后的构象变化的潜力，以便提供电子激发转换成信号转导所需的机械运动的详细图像。因此，该项目的理论发展有可能通过将已知的蛋白质结构与光诱导动力学相关联来揭示生物重要过程的最早机械步骤的光谱特征，以提供蛋白质功能的全面图片。