Posttranscriptional regulatory interactions during Herpes Simplex Virus infection

单纯疱疹病毒感染期间的转录后调控相互作用

• 批准号: 261502768
• 负责人: Professor Dr. Friedrich A. Grässer
• 金额: --
• 依托单位: Max-Delbrück-Centrum für Molekulare Medizin (MDC) in der Helmholtz-Gemeinschaft
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/261502768?language=en
• 关键词: Posttranscriptional; regulatory; interactions; during; Herpes

Herpes viruses cause a variety of human diseases with often very limited options for treatment or prevention. Thus, there is a need to improve our understanding of the replication strategies of these viruses to identify novel targets for intervention. Here, we want to analyze the dynamic interplay between mRNAs and proteins in Herpes Simplex Virus (HSV) -infected cells. We propose to systematically dissect posttranscriptional regulation during HSV infection concerning its molecular composition and architecture as well as its biological functions. We intend to discover host and viral mRNA-interacting proteins that show differential binding during viral replication, to identify their target transcripts and to characterize how these protein-RNA interactions influence the fate of cellular and viral mRNAs. Our results will be instrumental to integrate posttranscriptional interactions into other cellular gene regulatory networks as well as carry the potential for translational medicine by identifying novel RNA-binding proteins, which influence viral replication.

疱疹病毒引起多种人类疾病，但治疗或预防的选择往往非常有限。因此，有必要提高我们对这些病毒的复制策略的理解，以确定新的干预目标。在这里，我们想分析单纯疱疹病毒（HSV）感染细胞中mrna和蛋白质之间的动态相互作用。我们建议系统地剖析HSV感染过程中的转录后调控，包括其分子组成和结构以及生物学功能。我们打算发现在病毒复制过程中表现出差异结合的宿主和病毒mrna相互作用蛋白，鉴定它们的靶转录物，并表征这些蛋白质- rna相互作用如何影响细胞和病毒mrna的命运。我们的研究结果将有助于将转录后相互作用整合到其他细胞基因调控网络中，并通过鉴定影响病毒复制的新型rna结合蛋白，为转化医学提供潜力。