Auxiliary factors required for the accumulation and functioning of the thylakoid ATP synthase

类囊体 ATP 合酶积累和发挥作用所需的辅助因子

• 批准号: 261977268
• 负责人: Professor Dr. Dario Leister
• 金额: --
• 依托单位: Lehrstuhl für Molekularbiologie der Pflanzen
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/261977268?language=en
• 关键词: Auxiliary; factors; required; accumulation; functioning

The chloroplast F1Fo-ATP synthase (cpATPase) couples ATP synthesis to the light-driven electrochemical proton gradient and comprise two parts, the membranous CFo subcomplex that is a proton channel and the peripheral CF1 subcomplex that contains the catalytic sites for reversible ATP synthesis. To fully assemble a functional cpATPase complex, various auxiliary factors are needed - either for the expression of the chloroplast-located genes for cpATPase subunits (the atp genes) or for the assembly process itself. While several assembly factors of the F1Fo-ATP synthase are known in prokaryotes and yeast, for plants only the assembly factor ALB4 has been identified yet. In addition, a couple of plant proteins involved in atp gene expression have been described. In this project, we want to characterize two novel auxiliary proteins required for cpATPase biogenesis in the model plant Arabidopsis thaliana. The first one, AtCGL160, appears to be a true assembly factor and interacts physically with subunits of the CFo subcomplex. Interestingly, AtCGL160 contains besides a plant-specific domain also a shorter part, which is related to the prokaryotic Atp1/UncI proteins that have been also brought into connection with ATP synthase assembly. Therefore, we want to clarify the mechanism by which AtCGL160 promotes cpATPase assembly, whether this mechanism is conserved between prokaryotic Atp1/UncI proteins and eukaryotic CGL160 proteins, and reveal the function of the plant-specific part of AtCGL160. The second protein that we have identified to be required for cpATPase biogenesis is AtCGLD11. In contrast to AtCGL160, the AtCGLD11 protein is soluble and displays an mRNA expression pattern that suggests a function in atp gene expression. Therefore, we will characterise the function(s) of AtCGLD11 particularly in atp gene expression, including global approaches that can detect its interactions with any of the entire set of chloroplast genes and transcripts.

The chloroplast F1Fo-ATP synthase (cpATPase) couples ATP synthesis to the light-driven electrochemical proton gradient and comprise two parts, the membranous CFo subcomplex that is a proton channel and the peripheral CF1 subcomplex that contains the catalytic sites for reversible ATP synthesis.为了完全组装功能性的CPATPase复合物，需要各种辅助因子 - 用于CPATPase亚基（ATP基因）的叶绿体 - 偏置基因的表达，或用于组装过程本身。尽管在原核生物和酵母中已知F1FO-ATP合酶的几个组装因子，但对于植物，仅鉴定了装配因子Alb4。此外，已经描述了一些参与ATP基因表达的植物蛋白。在这个项目中，我们想表征在模型植物拟南芥中CPATPase生物发生所需的两种新型辅助蛋白。第一个ATCGL160似乎是一个真正的装配因子，并与CFO子复合物的亚基进行物理相互作用。有趣的是，ATCGL160除了植物特异性域外，还包含一个较短的部分，该域与原核ATP1/UNCI蛋白有关，该蛋白也已与ATP合酶组装有关。因此，我们要阐明ATCGL160促进CPATPase组装的机制，该机制是否在原核ATP1/UNCI蛋白和真核CGL160蛋白之间保守，并揭示ATCGL160植物特异性部分的功能。我们确定的第二种蛋白质是CPATPase生物发生所需的，是ATCGLD11。与ATCGL160相反，ATCGLD11蛋白是可溶的，并且显示出一种mRNA表达模式，该模式表明ATP基因表达的功能。因此，我们将表征ATCGLD11的功能，尤其是在ATP基因表达中，包括可以检测其与整个叶绿体基因和转录本的相互作用的全局方法。