Identification of plant RNA Editing factors in EMS mutants

EMS突变体中植物RNA编辑因子的鉴定

• 批准号: 262645124
• 负责人: Professor Dr. Stefan Binder, since 11/2017
• 金额: --
• 依托单位: Institut für Molekularbiologie und Biotechnologie der Prokaryoten
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/262645124?language=en
• 关键词: Identification; plant; RNA; Editing; factors

This project aims to identify further nuclear encoded factors of RNA editing in mitochondria of plants through the targeted genetic approach by screening EMS mutant populations of Arabidopsis thaliana. The successful identification of several new PPR genes and the novel family of MORF proteins by the SNaPshot mutant screen proves that this approach provides an excellent tool. The search includes the so far unknown enzyme and further genes for PPR and other proteins involved in RNA editing. Novel point mutations in known PPR proteins will allow to elucidate the functions of individual PPR protein domains. In parallel, functions of mitochondrially encoded proteins will be probed. Site-specific editing proteins will be employed to generate double knock-out and amiRNA knock-down mutants with specific alterations in mitochondrial proteins to assay their function, for example of the intronic matR reading frame.

本项目旨在通过筛选拟南芥EMS突变体群体，通过靶向遗传学方法进一步鉴定植物线粒体中RNA编辑的核编码因子。通过SNaPshot突变体筛选成功鉴定了几个新的PPR基因和新的MORF蛋白家族，证明这种方法提供了一个很好的工具。这项研究包括迄今为止未知的酶和PPR和其他参与RNA编辑的蛋白质的进一步基因。已知PPR蛋白中的新点突变将允许阐明单个PPR蛋白结构域的功能。与此同时，将探索神经编码蛋白的功能。将采用位点特异性编辑蛋白来产生在线粒体蛋白中具有特异性改变的双敲除和amiRNA敲低突变体，以测定它们的功能，例如内含子matR阅读框的功能。

项目成果

Two interacting PPR proteins are major Arabidopsis editing factors in plastid and mitochondria

• DOI: 10.1073/pnas.1705780114
• 发表时间: 2017-08-15
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Guillaumot, Damien;Lopez-Obando, Mauricio;Lurin, Claire
• 通讯作者: Lurin, Claire