In situ investigation of the interaction between water and small peptides on Au(110) and Cu(110) surfaces using near ambient pressure x-ray photoelectron spectroscopy (NAP-XPS) and reflection anisotropy spectroscopy (RAS)

使用近环境压力 X 射线光电子能谱 (NAP-XPS) 和反射各向异性光谱 (RAS) 原位研究 Au(110) 和 Cu(110) 表面上水和小肽之间的相互作用

• 批准号: 268794613
• 负责人: Professor Dr. Norbert Esser
• 金额: --
• 依托单位: Standort Berlin
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/268794613?language=en
• 关键词: situ; investigation; interaction; between; water

The goal of this project is to gain insight into the processes involved in interactions between water and biomolecules at metal surfaces. In particular, we want to elucidate the following questions regarding small biomolecules on metal surfaces: How do they adsorb to the surface? How do they interact at the surface? What is the role of water in these processes? And what is the influence of co-adsorbed metal ions? Therefore, we plan to study the interactions of water and small peptides on metal surfaces by investigating the effects of the surface structure and the environment (pressure, temperature, and pH) on these processes. This will include a study of the influence of water on the decomposition reactions of amino acids on metal surfaces. These phenomena will be studied with near-ambient pressure x-ray photoelectron spectroscopy (NAP-XPS), a new technique that allows us to study the surface structure at the molecular level in situ under more realistic pressure and temperature conditions. This study will be complemented by reflection anisotropy spectroscopy (RAS) measurements, providing information about bonding and orientation of adsorbed molecules on the surface. Other experimental methods will be used for further characterisation of the surfaces. These include LEED, STM, ellipsometry, and ir, and Raman spectroscopy. We will use single crystal surfaces of two metals: gold and copper as models for an inert and a reactive surface, respectively. The system of glutathione (g-Glu-Cys-Gly, GSH), its component amino acids, and the structurally related di-peptides g-glutamylcysteine (g-Glu-Cys) and L-cysteinylglycine (Cys-Gly) will be the main focus of our study. L-cysteine will be our control molecule, as the interaction of this species with both metal surfaces has been studied extensively.

该项目的目标是深入了解金属表面水和生物分子之间相互作用的过程。特别是，我们想阐明以下问题，关于金属表面上的小生物分子：它们如何吸附到表面？它们如何在表面相互作用？水在这些过程中的作用是什么？共吸附金属离子的影响是什么？因此，我们计划通过研究表面结构和环境（压力、温度和pH值）对这些过程的影响来研究水和小肽在金属表面的相互作用。这将包括研究水对金属表面氨基酸分解反应的影响。这些现象将通过近环境压力X射线光电子能谱（NAP-XPS）进行研究，这是一种新技术，使我们能够在更真实的压力和温度条件下原位研究分子水平的表面结构。这项研究将补充反射各向异性光谱（RAS）测量，提供有关表面上吸附分子的键合和取向的信息。其他实验方法将用于进一步表征的表面。这些包括LEED、STM、椭圆偏振、红外和拉曼光谱。我们将使用两种金属的单晶表面：金和铜分别作为惰性和活性表面的模型。谷胱甘肽（g-Glu-Cys-Gly，GSH）系统、其组成氨基酸以及结构相关的二肽g-谷氨酰半胱氨酸（g-Glu-Cys）和L-半胱氨酰甘氨酸（Cys-Gly）将成为我们研究的主要重点。L-半胱氨酸将是我们的控制分子，因为这种物质与两种金属表面的相互作用已被广泛研究。