Effect of phosphorylation on the archaellum regulatory protein network (P24*)

磷酸化对古菌调节蛋白网络的影响 (P24*)

• 批准号: 269889509
• 金额: --
• 依托单位: Forschungsschwerpunkt Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/269889509?language=en
• 关键词: Effect; phosphorylation; archaellum; regulatory; protein

Archaea use the archaellum, a motility structure that is assembled via a type IV pili mechanism, to swim. Expression of the archaellum is highly regulated and reversible protein phosphorylation of the membrane bound one component regulators ArnR and ArnR1 plays a major role in the modulation of the archaellum regulatory signaling protein network. We want to understand how phosphorylation alters the interactions in the archaellum regulatory network using biochemical, structural and in vivo approaches.

古生菌是一种通过IV型皮利机制组装起来的运动结构，它们利用这种结构来游泳。古菌的表达是高度调节的，并且膜结合的单组分调节剂ArnR和ArnR1的可逆蛋白磷酸化在古菌调节信号蛋白网络的调节中起主要作用。我们想了解磷酸化如何改变古菌调控网络中的相互作用，使用生物化学，结构和体内的方法。