小麦赤霉病及其病菌产生的真菌毒素严重威胁我国小麦生产和农产品质量安全。长江中下游和江淮稻麦轮作区，淹水稻田的土壤中易产生还原态铁离子（Fe2+），但没有对禾谷镰刀菌产生铁毒害，水旱轮作不影响病菌在土壤中的存活能力。项目前期研究发现，禾谷镰刀菌比常见真菌对铁胁迫有更强的适应性，且60个ABC蛋白中仅FgAtm1对铁胁迫超敏感。研究又发现FgAtm1缺失导致细胞内铁积累；FgHapX响应FgAtm1调控铁相关基因表达；FgHapX功能依赖两个位点的磷酸化；FgGrx4作为FgHapX的互作蛋白共同调控铁平衡。在此基础上，拟深入研究FgAtm1-FgHapX途径调控禾谷镰刀菌中铁平衡的分子机制，解析FgHapX磷酸化对其功能的影响，阐述FgHapX与FgGrx4互作的生物学意义，分析铁平衡在禾谷镰刀菌中的生物学功能。预期结果为真核生物中铁平衡调控提供新视点，为控制禾谷镰刀菌初菌源菌量提供新思路。

Fusarium graminearum complex as an economically important plant pathogen causes Fusarium head blight (FHB) on various cereal crops. Moreover, mycotoxins produced by the pathogen seriously threaten the health of human and animal. In recent years, wheat and rice were rotated in the middle and lower reaches of the Yangtze River and the regions of Jianghuai. However high iron toxicity did not affect the survival of F. graminearum in fields that were flooded. In our previous study, we found that F. graminearum displays increased tolerance to iron stress than other fungi and only FgAtm1 out of the 60 ABC transporters is involved in the regulation of iron homeostasis. Further studies showed that FgATM1 deletion caused intracellular iron accumulation, FgHapX controlled the transcription of iron-related genes that is responsive to FgAtm1 absence, the phosphorylation and interacting protein FgGrx4 is required for FgHapX function in iron homeostasis. Based on these previous results, this study will uncover the regulatory mechanism of iron homeostasis mediated by FgAtm1-FgHapX in F. graminearum, investigate the functions of the phosphorylation of FgHapX and FgGrx4 in modulating iron homeostasis, and explore the biological functions of iron homeostasis for F. graminearum. We expect that this study will provide a novel insight for regulation of iron homeostasis in eukaryotes and a new strategy for controlling the initial F. graminearum source in fields.