Label-free investigation of structure-dynamic relations of single membrane receptors using a tip-enhanced Raman scattering approach (A02)

使用尖端增强拉曼散射方法对单膜受体的结构动态关系进行无标记研究 (A02)

• 批准号: 279180144
• 金额: --
• 依托单位: Institut für Physikalische Chemie
• 依托单位国家: 德国
• 项目类别: CRC/Transregios
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/279180144?language=en
• 关键词: Label; free; investigation; structure; dynamic

This project will target specifically the development for a nanoscale and label-free structure-investigation of membrane receptors, potentially even more specific membrane receptor surfaces. By adapting tip-enhanced Raman spectroscopy (TERS) towards the specific requirements of biological membranes, a structural investigation based on vibrational spectroscopy will allow to identify single receptors and eventually unravel structure-function relations once these receptors undergo externally triggered changes. Ultimately, channeling events at a single receptor level can be tracked down under controlled environmental conditions.

该项目将专门针对膜受体（甚至可能是更具体的膜受体表面）的纳米级和无标记结构研究的开发。通过采用尖端增强拉曼光谱（TERS）来适应生物膜的特定要求，基于振动光谱的结构研究将能够识别单个受体，并最终在这些受体经历外部触发的变化时揭示结构-功能关系。最终，可以在受控环境条件下追踪单个受体水平的通道事件。