Mechanisms of neuropeptidergic regulation of larval settlement behavior in the marine worm, Platynereis dumerilii

海洋蠕虫 Platynereis dumerilii 幼虫沉降行为的神经肽调节机制

• 批准号: 279299140
• 负责人: Professor Dr. Gáspár Jékely
• 金额: --
• 依托单位: Centre for Organismal Studies (COS)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/279299140?language=en
• 关键词: Mechanisms; neuropeptidergic; regulation; larval; settlement

Marine invertebrate larval settlement and metamorphosis is an important transitional stage in the life cycle of several animal phyla. While in many cases this process is regulated by neuropeptides, how these molecules act within larvae to affect behavioral and morphological changes in a carefully timed manner is still unknown. In this project we will explore how larval settlement behavior is regulated by antagonistic neuropeptides. We will study the marine worm, Platynereis dumerilii, where we found that a conserved neuropeptide, myoinhibitory peptide (MIP) induces larval settlement behavior and this effect is antagonized by allatotropin/orexin signaling. Preliminary experiments suggest that MIP signals by free fatty acids to induce calcium spikes in the ciliated swimming cells of the larvae, resulting in a strong inhibition of ciliary beating and rapid settlement. Allatotropin acts antagonistically at an unknown point in the MIP signaling pathway to block the settlement-inducing effects of MIP signaling. We propose to characterize this signaling pathway through a combination of lipidomics, transcriptomics, behavioral and morphological approaches. The Platynereis system allows us to study detailed molecular signaling in a whole-organism context, linking specific molecules and behavior.

海洋无脊椎动物幼体的定居和变态是许多动物门生活史中的一个重要过渡阶段。虽然在许多情况下，这一过程是由神经肽调节的，但这些分子如何在幼虫体内以仔细定时的方式影响行为和形态变化仍然是未知的。在这个项目中，我们将探讨拮抗神经肽如何调节幼虫的定居行为。我们将研究海洋蠕虫，Platynereis dumerilii，在那里我们发现，一个保守的神经肽，肌抑制肽（MIP）诱导幼虫的定居行为，这种效果是由allatotropin/orexin信号拮抗。初步实验表明，MIP信号的游离脂肪酸诱导钙尖峰在纤毛游泳细胞的幼虫，导致强烈抑制纤毛跳动和快速解决。Allatotropin在MIP信号传导途径中的未知点处拮抗性地起作用以阻断MIP信号传导的沉降诱导作用。我们建议通过脂质组学，转录组学，行为和形态学方法的组合来表征这种信号通路。Platynereis系统使我们能够在整个生物体的背景下研究详细的分子信号，将特定的分子和行为联系起来。

项目成果

Dual signaling of Wamide myoinhibitory peptides through a peptide-gated channel and a GPCR in Platynereis

• DOI: 10.1096/fj.201800274r
• 发表时间: 2018-10-01
• 期刊: FASEB JOURNAL
• 影响因子: 4.8
• 作者: Schmidt, Axel;Bauknecht, Philipp;Jekely, Gaspar
• 通讯作者: Jekely, Gaspar

High Cell Diversity and Complex Peptidergic Signaling Underlie Placozoan Behavior

高细胞多样性和复杂的肽能信号传导是长生动物行为的基础

• DOI: 10.1016/j.cub.2018.08.067
• 发表时间: 2018
• 期刊: Current Biology
• 影响因子: 9.2
• 作者: Varoqueaux F;Williams EA;Truscello L;Grandemange S;Kamm K;Schierter B;Jékely G;Fasshauer D
• 通讯作者: Fasshauer D

Towards a systems-level understanding of development in the marine annelid Platynereis dumerilii.

对海洋环节动物 Platynereis dumerilii 发育的系统级理解

• DOI: 10.1016/j.gde.2016.07.005
• 发表时间: 2016
• 期刊: Current opinion in genetics & development
• 影响因子: 4
• 作者: Williams EA;Jékely G
• 通讯作者: Jékely G