Molecular genetic characterization of Curly Horse hair using high resolution marker sets and next generation sequencing data

这项研究的目的是研究基因变异和基因相互作用，在卷发的发展中发挥作用。在我们之前的分析中，我们能够检测到卷发的特定突变。表达分析表明，不同基因的相互作用决定了卷曲的松紧度和厚度。由于这些发现，我们将使用Mangaliza猪作为马的动物模型来研究这些相互作用，这些相互作用影响了季节性和温度依赖性的卷发变化。取样将在一个统一的环境中进行，每隔一个月取样一次，取样时间为F1和F2代的曼加利扎与德国大白鲨或德国白鲨交配。由于猪的世代间隔短，后代数量多，是一种合适的模型，从而为研究毛发发育提供了新的途径。此外，对Mangaliza品种特异性表型的遗传变异的鉴定有助于这种濒危品种的物种保护。

The aim of this study is to investigate genetic variants and gene interactions, which play a role in the development of curly hair. In our previous analyses we were able to detect specific mutations for curly hair. Expression analyses revealed that there are interactions of different genes involved in the determination of tightness and thickness of curls. Due to these findings, we are going to use Mangaliza pigs as an animal model for the horse to investigate these interactions that affect seasonal and temperature-dependent modifications of curly hair. The sampling is going to take place in a uniform environment, in monthly intervals and in F1 and F2 generations of Mangaliza x German Large White or German Lanrace matings. Due to the short generation interval and high number of progeny, the pig is an appropriate model and thus enables a new approach for research of hair development. In addition, the identification of a genetic variant responsible for the Mangaliza breed-specific phenotype contributes to species conservation of this endangered breed.