Molecular genetic characterization of Curly Horse hair using high resolution marker sets and next generation sequencing data

使用高分辨率标记集和下一代测序数据对卷曲马毛进行分子遗传学表征

• 批准号: 280354060
• 负责人: Professorin Dr. Julia Metzger
• 金额: --
• 依托单位: Institut für Tierzucht und Vererbungsforschung
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/280354060?language=en
• 关键词: Molecular; genetic; characterization; Curly; Horse

The aim of this study is to investigate genetic variants and gene interactions, which play a role in the development of curly hair. In our previous analyses we were able to detect specific mutations for curly hair. Expression analyses revealed that there are interactions of different genes involved in the determination of tightness and thickness of curls. Due to these findings, we are going to use Mangaliza pigs as an animal model for the horse to investigate these interactions that affect seasonal and temperature-dependent modifications of curly hair. The sampling is going to take place in a uniform environment, in monthly intervals and in F1 and F2 generations of Mangaliza x German Large White or German Lanrace matings. Due to the short generation interval and high number of progeny, the pig is an appropriate model and thus enables a new approach for research of hair development. In addition, the identification of a genetic variant responsible for the Mangaliza breed-specific phenotype contributes to species conservation of this endangered breed.

这项研究的目的是调查遗传变异和基因相互作用，这在卷发的发展中发挥作用。在我们之前的分析中，我们能够检测到卷发的特定突变。表达分析表明，不同基因之间存在相互作用，这些基因参与了卷曲紧密度和厚度的决定。由于这些发现，我们将使用Mangaliza猪作为马的动物模型，以研究这些影响卷发季节性和温度依赖性变化的相互作用。采样将在统一的环境中进行，每月一次，在Mangaliza x德国大白色或德国Lanrace交配的F1和F2代中进行。由于猪的世代间隔短，后代数量多，是一个合适的模型，从而为毛发发育的研究提供了一种新的途径。此外，负责Mangaliza品种特定表型的遗传变异的鉴定有助于物种保护这一濒危品种。