Uncovering the genetic basis for variation in adaptive responses to water deficit in Arabidopsis thaliana roots

揭示拟南芥根部对水分亏缺的适应性反应变异的遗传基础

• 批准号: 281317335
• 负责人: Dr. Heike Lindner
• 金额: --
• 依托单位: Department of Plant Biology
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/281317335?language=en
• 关键词: Uncovering; genetic; basis; variation; adaptive

Water deficit is a major stress for plants in nature, but relatively little is known about the molecular mechanisms roots use to adapt to this environmental condition, mainly due to the difficulty of simulating such conditions in the laboratory. GLO-Roots (Growth and Luminescence Observatory of Roots) offers the unique opportunity to visualize changes in root system architecture in soil-grown Arabidopsis thaliana plants under water deficit treatment. Preliminary data show that roots avoid drying soil by increasing gravity response and we hypothesize that this avoidance mechanism is diverse among a population of Arabidopsis thaliana accessions from different climatic areas. A genome wide association study will be performed to find the genetic basis of potential changes in root system architecture under water deficit. In addition, accessions with extreme phenotypes will be crossed and causative loci identified by bulk-segregant analysis and/or Quantitative Trait Loci-mapping. Lastly, to investigate if deeper root growth is mediated by stress-induced hormone signaling, characterization of the spatiotemporal dynamics of hormone-responive reporters and reverse genetic approaches will be conducted. In conclusion, we aim at revealing molecular mechanisms regulating changes in root system architecture in response to water deficit in Arabidopsis thaliana using the GLO-Roots system.

水分亏缺是自然界中植物的主要逆境，但人们对植物根系适应这种环境条件的分子机制知之甚少，这主要是因为在实验室模拟这种条件是困难的。Glo-Roots(根部生长和发光观测)提供了一个独特的机会，可以直观地观察在水分亏缺处理下土壤生长的拟南芥植物根系构型的变化。初步数据表明，根通过增加重力响应来避免土壤干燥，我们假设这种避免机制在来自不同气候地区的拟南芥种质群体中是不同的。将进行全基因组关联研究，以找出水分亏缺下根系构型潜在变化的遗传基础。此外，具有极端表型的材料将进行杂交，并通过批量分离分析和/或数量性状基因座定位来确定致病基因座。最后，为了研究更深层次的根生长是否受胁迫诱导激素信号的调节，我们将对激素反应报告的时空动态进行表征，并采用反向遗传方法。综上所述，我们旨在利用GLO-Roots系统揭示调控拟南芥根系构型变化以响应水分亏缺的分子机制。